1887

Abstract

Strains of peanut stunt cucumovirus (PSV) were classified into two distinct subgroups, I and II, based on Western and Northern blot analyses using antisera and cloned cDNA probes to strains PSV-ER and PSV-W. These results were corroborated by nucleotide sequence analyses of full-length cDNA clones of RNA3 from representative strains of the two subgroups. Whereas the percentage nucleotide sequence identity between PSV-ER (or PSV-J) and PSV-W RNA3s was determined to be 80%, the corresponding value between strains ER and J was 91%, confirming that strains ER and J belong to the same subgroup (subgroup I) whereas strain W belongs to a separate subgroup (subgroup II). PSV-W and PSV-ER RNA3s are 2173 and 2188 nucleotides long, respectively. Each is dicistronic, encoding a putative movement protein (3a protein) and a coat protein (CP). The intercistronic and 5′ untranslated region (UTR) sequences of PSV strains, unlike those of cucumber mosaic cucumovirus (CMV) strains, are highly conserved and thus not useful for distinguishing the two subgroups. However, the 3′ UTR sequences of PSV strains, like those of CMV strains, can discriminate between the two subgroups since strains within the same subgroup are 95% identical in their 3′ UTRs whereas those in different subgroups are only 74–78% identical. PSV-W and PSV-ER RNA4s were determined to be 994 and 1006 nucleotides long, respectively. PSV 3a and CP genes have higher percentage nucleotide sequence identities to those of tomato aspermy cucumovirus than to those of CMV.

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1997-04-01
2022-05-23
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References

  1. Boccard F., Baulcombe D. 1993; Mutational analysis of cis-acting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 193:563–578
    [Google Scholar]
  2. Davies C., Symons R. H. 1988; Further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus RNA 3. Virology 165:216–224
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of nucleotide sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Diaz-Ruiz J. R., Kaper J. M. 1983; Nucleotide sequence relationships among thirty peanut stunt virus isolates determined by competition hybridization. Archives of Virology 75:277–281
    [Google Scholar]
  5. Diaz-Ruiz J. R., Kaper J. M., Waterworth H. E., Devergne J. C. 1979; Isolation and characterization of peanut stunt virus from alfalfa in Spain. Phytopathology 69:504–509
    [Google Scholar]
  6. Ding S. -W., Anderson B. J., Haase H. R., Symons R. H. 1994; New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601
    [Google Scholar]
  7. Ghabrial S. A., Pickard C. M., Stuckey R. E. 1977; Identification and distribution of virus diseases of soybean in Kentucky. Plant Disease Reporter 61:690–694
    [Google Scholar]
  8. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  9. Hu C. -C., Ghabrial S. A. 1995; The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecylsulfate-polyacrylamide gels. Journal of Virological Methods 55:367–379
    [Google Scholar]
  10. Kaper J. M., Tousignant M. E., Diaz-Ruiz J. R., Tolin S. A. 1978; Peanut stunt virus-associated RNA5: second tripartite genome virus with an associated satellite-like replicating RNA. Virology 88:166–170
    [Google Scholar]
  11. Karasawa A., Nakaho K., Kakutani T., Minobe Y., Ehara Y. 1991; Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467
    [Google Scholar]
  12. Karasawa A., Nakaho K., Kakutani T., Minobe Y., Ehara Y. 1992; Nucleotide sequence analyses of peanut stunt cucumovirus RNAs 1 and 2. Journal of General Virology 73:701–707
    [Google Scholar]
  13. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. Lot H., Marrou J., Quiot J. B., Esvan C. 1972; Contributionà I’étude de virus de la mosalque du concombre (CMV). II. Méthode de purification rapide du virus. Annales de Phytopathologie 4:25–38
    [Google Scholar]
  16. McGarvey P., Tousignant M., Geletka L., Cellini F., Kaper J. M. 1995; The complete nucleotide sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs. Journal of General Virology 76:2257–2270
    [Google Scholar]
  17. Memelink J., Swords K. M. M., Staehelin L. A., Hoge J. H. C. 1994; Southern, Northern and Western blot analysis. In PlantMolecular Biology Manual, 2nd edn. pp. F1:1–23 Gelvin S. B., Schilperoort R. A. Edited by London: Kluwer Academic Publishers;
    [Google Scholar]
  18. Mink G. I. 1972 Peanut stunt virus CMI/AAB Descriptions of Plant Viruses 92
    [Google Scholar]
  19. Mink G. I., Silbernagel M. J., Saksena K. N. 1969; Host range, purification, and properties of the western strain of peanut stunt virus. Phytopathology 59:1625–1631
    [Google Scholar]
  20. Moriones E., Roossinck M. J., García-Arenal F. 1991; Nucleotide sequence of tomato aspermy virus RNA 2. Journal of General Virology 72:779–783
    [Google Scholar]
  21. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Archives of Virology 133:239–257
    [Google Scholar]
  22. Naidu R. A., Collins G. B., Ghabrial S. A. 1991a; Symptom- modulating properties of peanut stunt virus satellite RNA sequence variants. Molecular Plant-Microbe Interactions 4:268–275
    [Google Scholar]
  23. Naidu R. A., Collins G. B., Ghabrial S. A. 1991b; Nucleotide sequence analysis of a cDNA clone encoding the coat protein gene of peanut stunt virus. Plant Molecular Biology 17:175–177
    [Google Scholar]
  24. Naidu R. A., Collins G. B., Ghabrial S. A. 1992; Peanut stunt virus satellite RNA: analysis of sequences that affect symptom attenuation in tobacco. Virology 189:668–677
    [Google Scholar]
  25. Naidu R. A., Hu C. -C., Pennington R. E., Ghabrial S. A. 1995; Differentiation of eastern and western strains of peanut stunt cucumovirus based on satellite RNA support and nucleotide sequence homology. Phytopathology 85:502–507
    [Google Scholar]
  26. Nitta N., Masuta C., Kuwata S., Takanami Y. 1988; Comparative studies on the nucleotide sequence of cucumber mosaic virus RNA3 between Y strain and Q strain. Annals of the Phytopathological Society of Japan 54:516–522
    [Google Scholar]
  27. O’Reilly D., Thomas C. J. R., Coutts R. H. A. 1991; Tomato aspermy virus has an evolutionary relationship with other tripartite RNA plant viruses. Journal of General Virology 72:1–7
    [Google Scholar]
  28. Owen J., Shintaku M., Aeschleman P., Tahar S. B., Palukaitis P. 1990; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 3. Journal of General Virology 71:2243–2249
    [Google Scholar]
  29. Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. B. 1992; Cucumber mosaic virus. Advances in Virus Research 41:281–348
    [Google Scholar]
  30. Roossinck M. J., Sleat D., Palukaitis P. 1992; Satellite RNAs of plant viruses : structures and biological effects. Microbiological Reviews 56:265–279
    [Google Scholar]
  31. Rybicki E. P. 1995; The Bromoviridae. In Virus Taxonomy Sixth Report of the International Committee on Taxonomy of Viruses pp. 450–457 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  32. Salanki K., Balazs E., Burgyan J. 1994a; Nucleotide sequence and infectious in vitro transcripts of RNA 3 of tomato aspermy virus pepper isolate. Virus Research 33:281–289
    [Google Scholar]
  33. Salanki K., Thole V., Balazs E., Burgyan J. 1994b; Complete nucleotide sequence of the RNA 3 from subgroup II of cucumber mosaic virus (CMV) strain: Trk7. Virus Research 31:379–384
    [Google Scholar]
  34. Sambrook J., Fritch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  36. Shen W., Waye M. M. Y. 1988; A novel method for generating a nested set of unidirectional deletion mutants using mixed oligodeoxy-nucleotides. Gene 70:205–211
    [Google Scholar]
  37. Sippel A. E. 1973; Purification and characterization of adenosine triphosphate-ribonucleic acid adenyl transferase from Escherichia coli. European Journal of Biochemistry 37:31–40
    [Google Scholar]
  38. Vieira J., Messing J. 1987; Production of single stranded plasmid DNA. Methods in Enzymology 153:3–11
    [Google Scholar]
  39. Xu Z., Barnett O. W., Gibson P. B. 1986; Characterization of peanut stunt virus strains by host reactions, serology, and RNA patterns. Phytopathology 76:390–395
    [Google Scholar]
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