1887

Abstract

A detailed analysis of simian immunodeficiency virus (SIV)-specific cytotoxic T lymphocyte (CTL) responses and the identification of the proteins and epitopes they target may improve the design of immunotherapeutic interventions and provide insights into AIDS pathogenesis. Here, we identified a new CTL epitope in the SIV Gag protein, recognized by CD8 and MHC class I-restricted CTL clones from a long-term asymptomatic cynomolgus macaque () infected with SIVmac32H-J5. Using overlapping synthetic peptides, the optimal minimal epitope was characterized as a nine amino acid peptide representing amino acids 242–250 of p26 (SVDEQIQWM). CTL recognition was shown to be abolished by amino acid substitutions observed within homologous human immunodeficiency virus (HIV)-1 and HIV-2 sequences.

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1997-04-01
2024-04-25
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References

  1. Brander C., Pichler W. J., Corradin G. 1995; Identification of HIV protein-derived cytotoxic T lymphocytes (CTL) epitopes for their possible use as synthetic vaccine. Clinical and Experimental Immunology 101:107–113
    [Google Scholar]
  2. Chen Z, Shen L., Miller M. D., Ghim S. H., Hughes A. L., Letvin N. L. 1992; Cytotoxic T lymphocytes do not appear to select for mutations in an immunodominant epitope of simian immunodeficiency virus gag. Journal of Immunology 149:4060–4066
    [Google Scholar]
  3. Desrosiers R. 1990; The simian immunodeficiency viruses. Annual Review of Immunology 8: 557–578
    [Google Scholar]
  4. Franco A., Ferrari C., Sette A., Chisari F. V. 1995; Viral mutations, TCR antagonism and escape from the immune response. Current Opinion in Immunology 7: 524–531
    [Google Scholar]
  5. Geretti A. M., vanEls C. A. C. M., vanBaalen C. A., Poelen M. C. M., Osterhaus A. D. M. E. 1993; Preservation of phenotype and function of positively selected virus-specific CD8+ T lymphocytes following anti-Fab detachment from immunomagnetic beads. Journal of Immunological Methods 161:129–133
    [Google Scholar]
  6. Geretti A. M., Dings M. E. M., van Els C. A. C. M., van Baalen C. A., Wijnholds F. J., Borleffs J. C. C., Osterhaus A. D. M. E. 1996; HIV-1 and EBV specific cytotoxic T lymphocyte precursors exhibit different kinetics in HIV-1 infected persons. Journal of Infectious Diseases 174:34–45
    [Google Scholar]
  7. Hulskotte E. G. J., Geretti A. M., Siebelink K. H., van Amerongen G., Cranage M. P., Rud E. W., Norley S. G., de Vries P., Osterhaus A. D. M. E. 1995; Vaccine-induced virus-neutralizing antibodies and cytotoxic T cells do not protect macaques from experimental infection with simian immunodeficiency virus SIVmac 32H (J5). Journal of Virology 69: 6289–6296
    [Google Scholar]
  8. Letvin N. L., Reimann K. A., Yasutomi Y., Ringler D. J., Yamamoto Y. 1994; The SIVmac specific cytotoxic T lymphocyte response in the acutely infected rhesus monkey. Current Topics in Microbiology and Immunology 188:175–184
    [Google Scholar]
  9. Mills K. H. G., Jones W. C. 1994; CD4+ T cell responses to simian immunodeficiency virus. Current Topics in Microbiology and Immunology 188:161–173
    [Google Scholar]
  10. Myers G., Wain-Hobson S., Henderson L. E., Korber B., Jeang K., Pavlakis G. N. 1994 Human Retroviruses and AIDS : A Compilation and Analysis of Nucleic and Amino Acid Sequences Los Alamos National Laboratory Data Base, Los Alamos, N. Mex., USA:
    [Google Scholar]
  11. Rud E. W., Cranage M., Yon J., Quirk J., Ogilvie L., Cook N., Webster S., Dennis M., Clarke B. 1994; Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants. Journal of General Virology 75:529–543
    [Google Scholar]
  12. Van Baalen C. A., Klein M. R., Huisman R. C., Dings M. E. M., Kerkhof Garde S. R., Geretti A. M., Gruters R., Van Els C. A. C. M., Miedema F., Osterhaus A. D. M. E. 1996; Fine specificity of cytotoxic T lymphocytes which recognize conserved epitopes of the Gag protein of human immunodeficiency virus type 1. Journal of General Virology 77:1659–1665
    [Google Scholar]
  13. Van de Griend R. J., van Krimpen B. A., Bol S. J., Thompson A., Bolhuis R. L. 1984; Rapid expansion of human cytotoxic T cell clones: growth promotion by heat-labile serum component and by various types of feeder cells. Journal of Immunological Methods 66: 285–298
    [Google Scholar]
  14. Watkins D. I. 1994; MHC of nonhuman primates. Current Topics in Microbiology and Immunology 188:145–159
    [Google Scholar]
  15. Yasutomi Y., Koenig S., Woods R. M., Madsen J., Wassef N. M., Alving C. R., Klein H. J., Nolan T. E., Boots L. J., Kessler J. A., Emini E. A., Conley A. J., Letvin N. L. 1995; A vaccine-elicited, single viral epitope-specific cytotoxic T lymphocyte response does not protect against cell-free simian immunodeficiency virus challenge. Journal of Virology 69:2279–2284
    [Google Scholar]
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