1887

Abstract

The complete sequence of the 3454 nt of RNA 2 of the Ahlum isolate of beet soil-borne furovirus (BSBV) has been determined starting with two short stretches of cloned cDNA. Unknown parts of the sequence were amplified by means of RT-PCR techniques using combinations of specific and random primers. BSBV RNA 2 is more similar in its genetic organization to potato mop top virus (PMTV) RNA 3 than to any other furoviral RNA, although it is more than 1100 nt longer. Its 3 -end, unlike that of PMTV RNA 3, has the potential to fold into a tRNA-like structure. It contains one large open reading frame for a readthrough protein with a molecular mass of 104 kDa (104K protein) which is interrupted internally by an amber stop codon terminating the coding region for a protein of 19 kDa (19K), most likely the viral coat protein (CP). The readthrough domain of the 104K protein is much larger than that of PMTV, but the N- and C- proximal portions of these domains are similar for the two viruses. No serological relationships were found between the particles of the two viruses, although more than 50% of the amino acid sequences of the putative CPs are identical.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-2-469
1997-02-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/2/9018071.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-2-469&mimeType=html&fmt=ahah

References

  1. Bouzoubaa S., Quillet L., Guilley H., Jonard G., Richards K. 1987; Nucleotide sequence of beet necrotic yellow vein virus RNA-1. Journal of General Virology 68:615–626
    [Google Scholar]
  2. Chen J., MacFarlane S. A., Wilson T. M. A. 1994; Detection and sequence analysis of a spontaneous deletion mutant of soil-borne wheat mosaic virus RNA2 associated with increased symptom severity. Virology 202:921–929
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analyses for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, USA 79:5818–5822
    [Google Scholar]
  5. Gustafson G., Armour S. L. 1986; The complete nucleotide sequence of RNAa from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
    [Google Scholar]
  6. Haeberlé A. M., Stussi-Garaud C., Schmitt C., Garaud J. C., Richards K. E., Guilley H., Jonard G. 1994; Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles. Archives of Virology 134:195–203
    [Google Scholar]
  7. Hehn A., Bouzoubaa S., Bate N., Twell D., Marbach J., Richards K., Guilley H., Jonard G. 1995; The small cysteine-rich protein P14 of beet necrotic yellow vein virus regulates accumulation of RNA2 in cis and coat protein in trans. Virology 210:73–81
    [Google Scholar]
  8. Herzog E., Guilley H., Manohar S. K., Dollet M., Richards K., Fritsch C., Jonard G. 1994; Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. Journal of General Virology 75:3147–3155
    [Google Scholar]
  9. Hutchinson P. J., Henry C. M., Coutts R. H. A. 1992; A comparison, using dsRNA analysis, between beet soil-borne virus and some other tubular viruses isolated from sugar beet. Journal of General Virology 73:1317–1320
    [Google Scholar]
  10. Ivanovic M., Macfarlane I., Wood R. D. 1983; Viruses ofsugarbeet associated with Polymyxa betae. Annual Report of Rothamsted Experimental Station for 1982 pp. 189–190
    [Google Scholar]
  11. Kashiwazaki S., Scott K. P., Reavy B., Harrison B. D. 1995; Sequence analysis and gene content of potato mop top virus RNA 3: further evidence of heterogeneity in the genome organization of furoviruses. Virology 206:701–706
    [Google Scholar]
  12. Kaufmann A., Li Y., Koenig R., Breyel E., Maiss E., Lüddecke P., Commandeur U. 1992; Single- and double-stranded RNAs associated with an isolate of beet soil-borne virus. Intervirology 33:97–102
    [Google Scholar]
  13. Koenig R., Lüddecke P., Haeberlé A. M. 1995; Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products. Journal of General Virology 76:2051–2055
    [Google Scholar]
  14. Koenig R., Beier C., Commandeur U., Lüth U., Kaufmann A., Lüddecke P. 1996; Beet soil-borne virus RNA 3 - a further example for the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying RNAs. Virology 216:202–207
    [Google Scholar]
  15. Koonin E. V., Dolia V. V. 1993; Evolution and taxonomy of positive-strand RNA viruses: Implications of comparative analaysis of amino acid sequences. Critical Reviews in Biochemistry and Molecular Biology 28:375–430
    [Google Scholar]
  16. Lesemann D. -E., Koenig R., Lindsten K., Henry C. M. 1989; Serotypes of beet soil-borne furovirus from FRG and Sweden. Bulletin OEPP/EPPO Bulletin 19:539–540
    [Google Scholar]
  17. Manohar S. K., Guilley H., Dollet M., Richards K. E., Jonard G. 1993; Nucleotide sequence and genetic organization of peanut clump virus RNA 2 and partial characterization of deleted forms. Virology 195:33–41
    [Google Scholar]
  18. Matzura O., Wennborg A. 1996; RNAdraw: an integrated program for RNA secondary structure calculation and analysis under 32-bit Microsoft Windows. Cabios 12:247–249
    [Google Scholar]
  19. Milne R. G. 1984; Electron microscopy for the identification of plant viruses in in vitro preparations. Methods in Virology 787–120 Maramorosch K., Koprowski H. Edited by New York: Academic Press, Inc;
    [Google Scholar]
  20. Pereira L. G., Torrance L., Roberts I. M., Harrison B. D. 1994; Antigenic structure of the coat protein of potato mop-top virus. Virology 203:277–285
    [Google Scholar]
  21. Schmitt C., Balmori E., Jonard G., Richards K. E., Guilley H. 1992; In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly. Proceedings of the National Academy of Sciences, USA 89:5715–5719
    [Google Scholar]
  22. Scott K. P., Kashiwazaki S., Reavy B., Harrison B. D. 1994; The nucleotide sequence of potato mop-top virus RNA 2 : a novel type of genome organization for a furovirus. Journal of General Virology 75:3561–3568
    [Google Scholar]
  23. Shirako Y., Wilson T. M. A. 1993; Complete nucleotide sequence and organization of the bipartite RNA genome of soil-borne wheat mosaic virus. Virology 195:16–32
    [Google Scholar]
  24. Skuzeski J. M., Nichols L. M., Gesteland R. F., Atkins J. F. 1991; The signal for a leaky UAG stop codon in several plant viruses includes the two downstream codons. Journal of Molecular Biology 218:365–373
    [Google Scholar]
  25. Tamada T., Kusume T. 1991; Evidence that the 75K readthrough protein of beet necrotic yellow vein virus RNA-2 is essential for transmission by the fungus Polymyxa betae. Journal of General Virology 72:1497–1504
    [Google Scholar]
  26. Tamada T., Schmitt C., Saito M., Guilley H., Richards K., Jonard G. 1996; High resolution analysis of the readthrough domain of beet necrotic yellow vein virus readthrough protein: a KTER motif is important for efficient transmission of the virus by Polymyxa betae. Journal of General Virology 77:1359–1367
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-2-469
Loading
/content/journal/jgv/10.1099/0022-1317-78-2-469
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error