1887

Abstract

Tobacco rattle virus isolate PpK20 is transmitted by the nematode . RNA 2 of the virus determines vector transmissibility and encodes the viral coat protein and two non-structural proteins with molecular masses of 29·4 kDa and 32·8 kDa. Deletions and a frameshift in the two non-structural genes did not interfere with en- capsidation or co-replication of RNA 2 with RNA 1 after mechanical inoculation of plants. Mutations that affected the 29·4 K gene or both non-structural genes abolished nematode transmission, whereas a large deletion in the 32·8K gene had no effect on transmission by . It is concluded that the 29·4 K gene but not the 328K gene is involved in transmission of isolate PpK20 by this vector.

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/content/journal/jgv/10.1099/0022-1317-78-2-465
1997-02-01
2022-05-19
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