1887

Abstract

Tobacco rattle virus isolate PpK20 is transmitted by the nematode . RNA 2 of the virus determines vector transmissibility and encodes the viral coat protein and two non-structural proteins with molecular masses of 29·4 kDa and 32·8 kDa. Deletions and a frameshift in the two non-structural genes did not interfere with en- capsidation or co-replication of RNA 2 with RNA 1 after mechanical inoculation of plants. Mutations that affected the 29·4 K gene or both non-structural genes abolished nematode transmission, whereas a large deletion in the 32·8K gene had no effect on transmission by . It is concluded that the 29·4 K gene but not the 328K gene is involved in transmission of isolate PpK20 by this vector.

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/content/journal/jgv/10.1099/0022-1317-78-2-465
1997-02-01
2024-10-03
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References

  1. Angenent G. C., Linthorst H. J. M., van Belkum A. F., Cornelissen B. J. C., Bol J. F. 1986; RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14:4673–4682
    [Google Scholar]
  2. Brown D. J. F., Ploeg A. T., Robinson D. J. 1989; The association between serotypes of tobraviruses and Trichodorus and Paratrichodorus species. EPPO Bulletin 19:611–617
    [Google Scholar]
  3. Brown D. J. F., Robinson W. M., Trudgill D. L. 1995; Transmission of viruses by plant nematodes. Annual Review of Phytopathology 33:223–249
    [Google Scholar]
  4. Goulden M. G., Lomonossoff G. P., Davies J. W., Wood K. R. 1990; The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Research 18:4507–4512
    [Google Scholar]
  5. Hamilton W. D. O., Boccara M., Robinson D. J., Baulcombe D. C. 1987; The complete nucleotide sequence of tobacco rattle virus RNA-1. Journal of General Virology 68:2563–2575
    [Google Scholar]
  6. Hernández C., Mathis A., Brown D. J. F., Bol J. F. 1995; Sequence of RNA 2 of a nematode-transmissible isolate of tobacco rattle virus. Journal of General Virology 76:2847–2851
    [Google Scholar]
  7. Hernández C., Carette J., Brown D. J. F., Bol J. F. 1996; Serial passage of tobacco rattle virus under different selection conditions results in deletion of structural and nonstructural genes in RNA 2. Journal of Virology 70:4933–4940
    [Google Scholar]
  8. MacFarlane S. A., Brown D. J. F. 1995; Sequence comparison of RNA2 of nematode-transmissible and nematode-non-transmissible isolates of pea early-browning virus suggests that the gene encoding the 29 kDa protein may be involved in nematode transmission. Journal of General Virology 76:1299–1304
    [Google Scholar]
  9. MacFarlane S. A., Taylor S. C., King D. I., Hughes G., Davies J. W. 1989; Pea early browning virus RNA1 encodes four polypeptides including a putative zinc-finger protein. Nucleic Acids Research 17:2245–2260
    [Google Scholar]
  10. MacFarlane S. A., Brown D. J. F., Bol J. F. 1995; The transmission of nematodes by tobraviruses is not determined exclusively by the viral coat protein. European Journal of Plant Pathology 101:535–539
    [Google Scholar]
  11. MacFarlane S. A., Wallis C. V., Brown D. J. F. 1996; Multiple virus genes involved in the nematode transmission of PEBV. Virology 219:417–422
    [Google Scholar]
  12. Pirone T. P. 1991; Viral genes and gene products that determine insect transmissibility. Seminars in Virology 2:81–87
    [Google Scholar]
  13. Ploeg A. T., Robinson D. J., Brown D. J. F. 1993; RNA-2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid vector nematodes. Journalof General Virology 74:1463–1466
    [Google Scholar]
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