1887

Abstract

The causative agent of Taura syndrome (TS) was recognized in 1994 to be viral in nature and tentatively classified as belonging to either the family or . The work reported here has led to a more definitive classification of this new penaeid virus. Located within the cytoplasm of infected cuticular epithelial cells of penaeid shrimp, the virus is a 31 to 32 nm icosahedral particle with a buoyant density of 1·338 ± 0·001 g/ml. Three major (55, 40 and24 kDa) and one minor (58 kDa) polypeptides constitute its proteinic capsid. Its genome contains a single molecule of ssRNA, which is polyadenylated at the 3′ end and approximately 9 kb in length. Based on these characteristics, we believe that TS virus should be included in the family Ecuadorian and Hawaiian TS virus isolates were found to be identical in their biophysical, biochemical and biological characteristics, and should be considered as the same virus.

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1997-02-01
2024-12-03
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References

  1. Bell T. A., Lightner D. V. 1987; IHHN disease of Penaeus stylirostris: effects of shrimp size on disease expression. Journal of Fish Diseases 10:165–170
    [Google Scholar]
  2. Bonami J. -R., Lightner D. V. 1991; Unclassified viruses of crustacea. In Atlas of Invertebrate Viruses pp 597–622 Adams J. R., Bonami J. R. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  3. Bonami J. -R., Trumper B., Mari J., Brehelin M., Lightner D. V. 1990; Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps. Journal of General Virology 71:2657–2664
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Brock J. A., Gose R., Lightner D. V., Hasson K. 1995; An overview on Taura syndrome, an important disease of farmed Penaeus vannamei. . In Swimming Through Troubled Water.Proceedings of the Special Session on Shrimp Farming pp 84–94 Browdy C. L., Hopkins J. S. Edited by Baton Rouge: World Aquaculture Society;
    [Google Scholar]
  6. Giles K. W., Myers A. 1965; An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature 206:95
    [Google Scholar]
  7. Hasson K. W., Lightner D. V., Poulos B. T., Redman R. M., White B. L., Brock J. A., Bonami J. -R. 1995; Taura syndrome in Penaeus vannamei: demonstration of a viral etiology. Diseases ofAquatic Organisms 23:115–126
    [Google Scholar]
  8. Jimenez R. 1992; ‘Syndrome de Taura’ (resumen). Aquacultura del Ecuador, Guayaquil 1:1–16
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  10. Lightner D. V. 1996 A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp Baton Rouge: World Aquaculture Society;
    [Google Scholar]
  11. Lightner D. V., Redman R. M., Hasson K. W., Pantoja C. R. 1995; .Taura syndrome in Penaeus vannamei (Crustacea: Decapoda): gross signs, histopathology and ultrastructure. Diseases of Aquatic Organisms 21:53–59
    [Google Scholar]
  12. Mejbaum W. Z. 1939; Üer Pentosenmengen, insbesonder in Derivaten der Adenylsaüre. Zeitschrift für Physiologische Chemie 258:117–120
    [Google Scholar]
  13. Moore N. F., Eley S. M. 1991; Picornaviridae: picornaviruses of invertebrates. In Atlas of Invertebrate Viruses pp 371–386 Adams J. R., Bonami J. R. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  14. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. 1995 Virus Taxonomy.Sixth Report of the International Committee on Taxonomy of Viruses Wien & New York: Springer Verlag;
    [Google Scholar]
  15. Pruder G. D., Brown C. L., Sweeney J. N., Carr W. H. 1995; High health shrimp systems; seed supply - theory and practice. In Swimming Through Troubled Waters Proceedings of the Special Session on Shrimp Farming, pp 40–52 Browdy C. L., Hopkins J. S. Edited by Baton Rouge: World Aquaculture Society;
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Weber K. O., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  18. Williams R. R., Dehdashti B., Lightner D. V. 1992; Performance of an aquatic multispecies system in evaluating the effects of a model microbial pest control agent on nontarget organisms. Journal of Toxicology and Environmental Health 37:247–264
    [Google Scholar]
  19. Wilson C. M. 1983; Staining of proteins on gels : comparison of dyes and procedures. Methods in Enzymology 91:236–274
    [Google Scholar]
  20. Wyban J. A., Swingle J. S., Sweeney J. N., Pruder G. D. 1992; Development and commercial performance of high health shrimp using specific pathogen free (SPF) broodstock Penaeus vannamei . Proceedings of the Special Session on Shrimp Farming pp 254–259
    [Google Scholar]
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