1887

Abstract

In order to locate amino acid residues involved in the formation of feline calicivirus (FCV) neutralizing epitopes, we analysed the capsid protein gene of monoclonal antibody neutralization-resistant variants of FCV. Amino acid substitutions in the variants were identified in the two hypervariable regions of the capsid protein. Four linear and two conformational epitopes were located in the regions from residues 426 to 460 and 490 to 520, respectively. The relative positions of individual epitopes agreed with our previous antigenic analy- sis.Two antigenic sitescomposed of the neutralizing epitopes were mapped in the hypervariable regions of the capsid protein, demonstrating that a relationship exists between the genetic variability and antigenic differences in the neutralization of FCV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-2-303
1997-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/2/9018050.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-2-303&mimeType=html&fmt=ahah

References

  1. Bachrach H. L., Hess W. R. 1973; Animal picornaviruses with a single major species of capsid protein. Biochemical and Biophysical Research Communications 55:141–149
    [Google Scholar]
  2. Burroughs J. N., Brown F. 1974; Physico-chemical evidence for the re-classification of the caliciviruses. Journal of General Virology 22:281–286
    [Google Scholar]
  3. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  4. Dawson S., McArdle F., Bennett M., Carter M., Milton I. P., Turner P., Meanger J., Gaskell R. M. 1993; Typing of feline calicivirus isolates from different clinical groups by virus neutralisation tests. Veterinary Record 133:13–17
    [Google Scholar]
  5. Gillespie J. H., Scott F. W. 1973; Feline viral infections. Advances in Veterinary Science and Comparative Medicine 17:163–200
    [Google Scholar]
  6. Guiver M., Littler E., Caul E. O., Fox A. J. 1992; The cloning, sequencing and expression of a major antigenic region from the feline calicivirus capsid protein. Journal of General Virology 73:2429–2433
    [Google Scholar]
  7. Kalunda M., Lee K. M., Holmes D. F., Gillespie J. H. 1975; .Serologic classification of feline caliciviruses by plaque-reduction neutralization and immunodiffusion. American Journal of Veterinary Research 36:353–356
    [Google Scholar]
  8. Milton I. D., Turner J., Teelan A., Gaskell R., Turner P. C., Carter M. J. 1992; Location of monoclonal antibody binding sites in the capsid protein of feline calicivirus. Journal of General Virology 73:2435–2439
    [Google Scholar]
  9. Povey R. C. 1974; Serologic relationships among feline caliciviruses. Infection and Immunity 10:1307–1314
    [Google Scholar]
  10. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  11. Seal B. S. 1994; Analysis of capsid protein gene variation among divergent isolates of feline calicivirus. Virus Research 33:39–53
    [Google Scholar]
  12. Seal B. S., Ridpath J. F., Mengeling W. L. 1993; Analysis of feline calicivirus capsid protein genes : identification of variable antigenic determinant regions of the protein. Journal of General Virology 74:2519–2524
    [Google Scholar]
  13. Shin Y. -S., Tohya Y., Oshikamo R., Kawaguchi Y., Tomonaga K., Miyazawa T., Kai C., Mikami T. 1993; Antigenic analysis of feline calicivirus capsid precursor protein and its deleted polypeptides produced in a mammalian cDNA expression system. Virus Research 30:17–26
    [Google Scholar]
  14. Tohya Y., Taniguchi Y., Tsubakimoto M., Takahashi E., Mikami T. 1990; Preparation and characterization of neutralizing monoclonal antibodies to feline calicivirus. Japanese Journal of Veterinary Science 52:251–256
    [Google Scholar]
  15. Tohya Y., Masuoka K., Takahashi E., Mikami T. 1991; Neutralizing epitopes of feline calicivirus. Archives of Virology 117:173–181
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-2-303
Loading
/content/journal/jgv/10.1099/0022-1317-78-2-303
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error