Development of a model for cytomegalovirus infection of oligodendrocytes Free

Abstract

The well-characterized human oligodendroglioma (HOG) cell line, cells of which resemble immature oligodendrocytes, was used to investigate the level of permissiveness to human cytomegalovirus (CMV) infection. Expression of CMV genes was incomplete following exposure of HOG cells to CMV, in contrast with results observed with the astroglioma cell line U373-MG (used as a positive control). However, treatment with phorbol 12-myristate 13-acetate (PMA) or with dibutryl cAMP (dbcAMP) plus the phosphatase inhibitor 1-isobutyl-3,3-methyl xanthine (IBMX) rendered the HOG cells fully permissive to CMV; down-regulation of HLA class I and production of virions were only observed under these conditions. In contrast to the findings seen with the HOG cell line, treatment of U373-MG cells with dbcAMP/IBMX or PMA did not interfere with CMV- induced down-regulation of HLA class I. However, these chemical stimulators reduced virus production in U373-MG cells by 30 and 70%, respectively.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-12-3349
1997-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/12/9400987.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-12-3349&mimeType=html&fmt=ahah

References

  1. Ahn K., Angulo A., Ghazal P., Peterson P. A., Yang Y., Fruh K. 1996; Human cytomegalovirus inhibits antigen presentation by a sequential multistep process. Proceedings of the National Academy of Sciences, USA 93:10990–10995
    [Google Scholar]
  2. Balluz I. M., Farrel M. A., Kay E., Staunton M. J., Keating J. N., Sheils O., Cosby S. L., Mabruk M. J., Sheahan B. J., Atkins G. J. 1996; Colocalization of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients. Irish Journal of Medical Science 165:133–138
    [Google Scholar]
  3. Bevan I. S., Daw R. A., Day P. J., Ala F. A., Walker M. R. 1991; Polymerase chain reaction for detection of human cytomegalovirus infection in a blood donor population. British Journal of Haematology 78:94–99
    [Google Scholar]
  4. Cranage M. P., Kuzarides T., Bankier A. T., Satchwell S., Weston K., Tomlinson P., Barrell B., Hart H., Bell S. E., Minson A. C., Smith G. L. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
    [Google Scholar]
  5. Dorfman L. 1973; Cytomegalovirus encephalitis in adults. Neurology 23:136–144
    [Google Scholar]
  6. Dutko F. J., Oldstone M. B. A. 1981; Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. Journal of Experimental Medicine 154:1636–1651
    [Google Scholar]
  7. Forbes B. A., Bonville C. A., Dock N. L. 1990; The effects of a promoter of cell differentiation and selected hormones on human cytomegalovirus infection using an in vitro cell system. Journal of Infectious Diseases 162:39–45
    [Google Scholar]
  8. Guyotat D., Gibert R., Chomal R., Archimbaud E., Bossard S., Maupas J., Fiere D., Aymard M. 1987; Incidence and prognosis of cytomegalovirus infection following allogenic bone marrow transplantation. Journal of Medical Virology 23:393–399
    [Google Scholar]
  9. Hengel H., Flohr T., Hammerling G. J., Koszinowski U. H., Momburg F. 1996; Human cytomegalovirus inhibits peptide translocation into the endoplasmic reticulum for MHC class I assembly. Journal of General Virology 77:2287–2296
    [Google Scholar]
  10. Ibanez C. E., Schrier R., Ghazal P., Wiley C., Nelson J. A. 1991; Human cytomegalovirus productively infects primary differentiated macrophages. Journal of Virology 65:6581–6588
    [Google Scholar]
  11. Jacobson M. A., Mills J. 1988; Serious cytomegalovirus disease in the acquired immunodeficiency syndrome. Annals of Internal Medicine 108:585–594
    [Google Scholar]
  12. Kashima T., Tiu S. N., Merrill J. E., Vinters H. V., Dawson G., Campagnoni A. T. 1993; Expression of oligodendrocyte-associated genes in cell lines derived from human gliomas and neuroblastomas. Cancer Research 53:170–175
    [Google Scholar]
  13. Koval V., Clark C., Vaishnav M., Spector S. A., Spector D. H. 1991; Human cytomegalovirus inhibits human immunodeficiency virus replication in cells productively infected with both viruses. Journal of Virology 65:6969–6978
    [Google Scholar]
  14. Kuntz-Simon G., Obert G. 1995; Sodium valproate, an anticonvulsant drug, stimulates human cytomegalovirus replication. Journal of General Virology 76:1409–1415
    [Google Scholar]
  15. La Femina T., Hayward G. S. 1986; Constitutive and retinoic-acid inducible expression of cytomegalovirus immediate-early genes in human teratocarcinoma cells. Journal of Virology 58:434–440
    [Google Scholar]
  16. Morgello S., Cho E. S., Nielson S., Devinsky O., Petito C. K. 1987; Cytomegalovirus encephalitis in patients with acquired immunodeficiency syndrome: an autopsy study of 30 cases and a review of the literature. Human Pathology 18:289–297
    [Google Scholar]
  17. Nakervis G. A., Kumar M. L., Cox F. E., Gold E. 1984; A prospective study of maternal cytomegalovirus infection and its effect on the fetus. American Journal of Obstetrics and Gynecology 149:435–440
    [Google Scholar]
  18. Patchell R. A., White C. L., Clark A. W., Beschorna W. E., Santos G. W. 1985; Neurological complications of bone marrow transplantation. Neurology 23:136–144
    [Google Scholar]
  19. Poland S. D., Costello P., Dekaban G. A., Rice G. P. A. 1990; Cytomegalovirus in the brain: in vitro infection of human brain-derived cells. Journal of Infectious Diseases 162:1252–1262
    [Google Scholar]
  20. Poland S. D., Bambrick L. L., Dekaban G. A., Rice G. A. 1994; The extent of human cytomegalovirus replication in primary neurons is dependent on host cell differentiation. Journal of Infectious Diseases 170:1267–1271
    [Google Scholar]
  21. Post G. R., Dawson G. 1992; Characterization of a cell line derived from a human oligodendroglioma. Molecular and Chemical Neuropathology 16:303–317
    [Google Scholar]
  22. Power C., Poland S. D., Blume W. T., Birvin J. P., Rice G. P. 1990; Cytomegalovirus and Rasmussen’s encephalitis. Lancet 336:1282–1284
    [Google Scholar]
  23. Raible D. W., McMorris F. A. 1993; Oligodendrocyte differentiation and progenitor cell proliferation are independently regulated by cyclic AMP. Journal of Neuroscience Research 34:287–294
    [Google Scholar]
  24. Raine C. S., Fields B. N. 1973; Neurotropic viruses and the developing brain. New York State Journal of Medicine 73:1169–1179
    [Google Scholar]
  25. Schmidbauer M., Budka H., Ulrich W., Ambros P. 1989; Cytomegalovirus disease of the brain in AIDS and connatal infection: a comparative study by histology, immunocytochemistry, and in situ DNA hybridization. Journal of Neuropathology and Experimental Neurology 48:645–652
    [Google Scholar]
  26. Spiller O. B., Tufaro F., Morgan B. P., Devine D. V. 1996a; Altered expression of host-encoded complement regulators on human cytomegalovirus-infected cells. European Journal of Immunology 26:1532–1536
    [Google Scholar]
  27. Spiller O. B., Moretto G., Kim S. U., Morgan B. P., Devine D. V. 1996b; Complement expression on astrocytes and astrocytoma cell lines : failure of complement regulation at the C3 level correlates with very low CD55 expression. Journal of Neuroimmunology 71:97–106
    [Google Scholar]
  28. Tanaka J., Kamiya S., Ogura T., Sato H., Ogura H., Hatano M. 1985; Effect of dimethyl sulfoxide on interaction of human cytomegalovirus with host cell: conversion of a nonproductive state of cell to a productive state for virus replication. Virology 146:165–176
    [Google Scholar]
  29. Tanaka J., Sadanari H., Sato H., Fukuda S. 1991; Sodium butyrate-inducible replication of human cytomegalovirus in a human epithelial cell line. Virology 185:271–280
    [Google Scholar]
  30. Turtinen L. W., Seufzer B. J. 1994; Selective permissiveness of TPA differentiated THP-1 myelomonocytic cells for human cytomegalovirus strains AD169 and Towne. Microbial Pathogenesis 16:373–378
    [Google Scholar]
  31. Weinshenker B. G., Silton S., Rice G. P. 1988; Phorbol-ester induced differentiation permits productive human cytomegalovirus infection in a monocytic cell line. Journal of Immunology 140:1625–1631
    [Google Scholar]
  32. Wiertz E. J., Jones T. R., Sun L., Bogyo M., Geuze H. J., Ploegh H. 1996; The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol. Cell 84:769–779
    [Google Scholar]
  33. Wiley C. A., Nelson J. A. 1988; Role of human immunodeficiency virus and cytomegalovirus in AIDS encephalitis. American Journal of Pathology 133:73–81
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-12-3349
Loading
/content/journal/jgv/10.1099/0022-1317-78-12-3349
Loading

Data & Media loading...

Most cited Most Cited RSS feed