1887

Abstract

A field strain of Sendai virus (SeV) Ohita-M1 (M1) was isolated from an epidemic in an animal laboratory by passaging in mice. A mutant strain, Ohita- MVC11 (MVC11), was then obtained by passaging M1 in rhesus monkey (LLC-MK) cells. MVC11 was adapted to LLC-MK cells and produced 20 times higher levels of infectious virus than M1. This increased production of infectious virus in LLC-MK cells was associated with enhanced viral gene expression. However, MVC11 could not replicate efficiently in mouse lung and was not lethal to mice even when inoculated at a titre of 8 × 10 cell-infecting units (CIU) per mouse. On the other hand, with an inoculum of only 4 × 10 CIU per mouse, corresponding to 1 LD, M1 replicated well in mouse lung and was highly virulent to mice. Nucleotide and deduced amino acid sequence analyses of the entire genomes of M1 and MVC11 revealed that adaptation to LLC-MK cells and the attenuation of mouse pathogenicity of MVC11 were associated with only two amino acid substitutions; one on the C protein (Phe substituted by Ser at position 170) and the other on the RNA polymerase, the L protein (Glu substituted by Ala at position 2050).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-12-3207
1997-12-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/12/9400971.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-12-3207&mimeType=html&fmt=ahah

References

  1. Bhatt P. N., Jonas A. M. 1974; An epizootic of Sendai virus infection with mortality in a barrier-maintained mouse colony. American Journal of Epidemiology 100:222–229
    [Google Scholar]
  2. Brown E. G. 1990; Increased virulence of a mouse-adapted variant of influenza A/FM/l/47 virus is controlled by mutations in genome segments 4, 5, 7, and 8. Journal of Virology 64:4523–4533
    [Google Scholar]
  3. Brownstein D. G. 1983; Genetics of natural resistance to Sendai virus infection in mice. Infection and Immunity 41:308–312
    [Google Scholar]
  4. Burek J. D., Zurcher C., Van Nunen M. C. J., Hollander C. F. 1977; A naturally occurring epizootic caused by Sendai virus in breeding and aging rodent colonies. II. Infection in the rat. Laboratory Animal Science 27:963–971
    [Google Scholar]
  5. Cadd T., Garcin D., Tappel C., Itoh M., Homma M., Roux L., Curran J. , Kolakofsky D. 1996; The Sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion. Journal of Virology 70:5067–5074
    [Google Scholar]
  6. Cao J.-X., Haolin N., Wills M. R., Campbell G. A., Sil B. K., Ryman K. D., Kitchen I., Barrett A. D. T. 1995; Passage of Japanese encephalitis virus in HeLa cells results in attenuation of virulence in mice. Journal of General Virology 76:2757–2764
    [Google Scholar]
  7. Curran J., De Melo M., Moyer S., Kolakofsky D. 1991; Characterization of the Sendai virus V protein with an anti-peptide antiserum. Virology 184:108–116
    [Google Scholar]
  8. Curran J., Marq J.-B., Kolakofsky D. 1992; The Sendai virus nonstructural C proteins specifically inhibit viral mRNA synthesis. Virology 189:647–656
    [Google Scholar]
  9. Fujita K., Silver J., Peden K. 1992; Changes in both gp120 and gp41 can account for increased growth potential and expanded host range of human immunodeficiency virus type 1. Journal of Virology 66:4445–4451
    [Google Scholar]
  10. Graff J., Normann A., Feinstone S. M., Flehmig B. 1994; Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants. Journal of Virology 68:548–554
    [Google Scholar]
  11. Hidaka Y., Kanda T., Iwasaki K., Nomoto A., Shioda T., Shibuta H. 1984; Nucleotide sequence of a Sendai virus genome region covering the entire M gene and the 3ʹ proximal 1013 nucleotides of the F gene. Nucleic Acids Research 12:7965–7973
    [Google Scholar]
  12. Ishida N., Homma M. 1978; Sendai virus. Advances in Virus Research 23:349–383
    [Google Scholar]
  13. Itoh T., Kagiyama N., Iwai H., Okada O., Takashina S., Gotoh H. 1978; Sendai virus infection in a small mouse breeding colony. Japanese Journal of Veterinary Science 40:615–618
    [Google Scholar]
  14. Itoh M., Homma M. 1987; Singl e amino acid substitution of Sendai virus at the cleavage site of the fusion protein confers trypsin resistance. Journal of General Virology 68:2939–2944
    [Google Scholar]
  15. Itoh M., Tan D.-M., Hayashi T., Mochizuki Y., Homma M. 1990; Pneumopathogenicity of a Sendai virus protease-activation mutant, TCs, which is sensitive to trypsin and chymotrypsin. Journal of Virology 64:5660–5664
    [Google Scholar]
  16. Itoh M., Wang X.-L., Suzuki Y., Homma M. 1992; Mutation of the HANA protein of Sendai virus by passaging in eggs. Virology 190:356–364
    [Google Scholar]
  17. Iwai H., Itoh T., Shumiya S. 1977; Persistence of Sendai virus infection in a mouse breeder colony and possibility to establish the virus free colony. Experimental Animals Tokyo 26:205–212
    [Google Scholar]
  18. Iwai H., Itoh T., Kagiyama N., Nomura T. 1980; Monitoring of murine infections in facilities for animal experimentation. In Animal Quality and Models in Biomedical Research pp 219–222 Spiegel A., Erichsen S., Solleveld H. A. Edited by Stuttgart: Gustav Fischer Verlag;
    [Google Scholar]
  19. Kashiwazaki H., Homma M., Ishida N. 1965; Assay of Sendai virus by immunofluorescence and hemadsorbed cell-counting procedures. Proceedings of the Society for Experimental Biology and Medicine 120:134–138
    [Google Scholar]
  20. Kato A., Kiyotani K., Sakai Y., Yoshida T., Nagai Y. 1997; Importance of the V protein for determining in vitro phenotypes and in vivo pathogenicity of Sendai virus. EMBO Journal 16:578–587
    [Google Scholar]
  21. Lamb R. A., Kolakofsky D. 1996; Paramyxoviridae: the viruses and their replication. In Fields Virology, 3rd edn. pp 1177–1204 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  22. Lucas C., Frie S., Peters R., Parker J. 1987; A quantitative immunofluorescence test for detection of serum antibody to Sendai virus in mice. Laboratory Animal Science 37:51–54
    [Google Scholar]
  23. Middleton Y., Tashiro M., Thai T., Oh J., Seymour J., Pritzer E., Klenk H.-D., Rott R., Seto J. 1990; Nucleotide sequence analyses of the genes encoding the HN, M, NP, P and L proteins of two host range mutants of Sendai virus. Virology 176:656–657
    [Google Scholar]
  24. Mizumoto K., Muroya K., Takagi T., Omata-Yamada T., Shibuta H., Iwasaki K. 1995; Protein factors required for in vitro transcription of Sendai virus genome. Journal of Biochemistry 117:527–534
    [Google Scholar]
  25. Moyer S. A., Baker S. C., Lessard J. L. 1986; Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNA. Proceedings of the National Academy of Sciences USA: 835405–5409
    [Google Scholar]
  26. Omata-Yamada T., Hagiwara K., Katoh K., Yamada H., Iwasaki K. 1988; Purification of the Sendai virus nonstructural C protein expressed in E. coli, and preparation of antiserum against C protein. Archives of Virology 103:61–72
    [Google Scholar]
  27. Parker J. C., Reynolds R. K. 1968; Natural history of Sendai virus infection in mice. American Journal of Epidemiology 88:112–125
    [Google Scholar]
  28. Parker J. C., Whitman M. D., Richter C. B. 1978; Susceptibility of inbred and outbred mouse strains to Sendai virus and prevalence of infection in laboratory rodents. Infection and Immunity 19:123–130
    [Google Scholar]
  29. Profeta M. L., Lief F. S., Plotkin S. A. 1969; Enzootic Sendai infection on laboratory hamsters. American Journal of Epidemiology 89:316–324
    [Google Scholar]
  30. Radecke F., Billeter M. A. 1996; The nonstructural C protein is not essential for multiplication of Edmonston B strain measles virus in cultured cells. Virology 217:418–421
    [Google Scholar]
  31. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 23:493–497
    [Google Scholar]
  32. Rubin S. A., Waltrip R. W.II Bautista J. R., Carbone K. M. 1993; Borna disease virus in mice: host-specific difference in disease expression. Journal of Virology 67:548–552
    [Google Scholar]
  33. Sakaguchi T., Kiyotani K., Sakaki M., Fujii Y., Yoshida T. 1994; A field isolate of Sendai virus: its high virulence to mice and genetic divergence from prototype strains. Archives of Virology 135:159–164
    [Google Scholar]
  34. Sawyer L. S. W., Wrin M. T., Crawford-Miksza L., Potts B., Wu Y., Weber P. A., Alfonso R. D., Hanson C. V. 1994; Neutralization sensitivity of human immunodeficiency virus type 1 is determined in part by the cell in which the virus is propagated. Journal of Virology 68:1342–1349
    [Google Scholar]
  35. Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K. 1983; Sequence of 3687 nucleotides from the 3ʹ end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Research 11:7317–7330
    [Google Scholar]
  36. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  37. Tashiro M., Homma M. 1983; Pneumotropism of Sendai virus in relation to protease-mediated activation in mouse lungs. Infection and Immunity 39:879–888
    [Google Scholar]
  38. Tashiro M., Seto J. T., Choosakul S., Yamakawa M., Klenk H.-D., Rott R. 1992; Budding site of Sendai virus in polarized epithelial cells is one of the determinants for tropism and pathogenicity in mice. Virology 182:413–422
    [Google Scholar]
  39. Wang X.-L., Itoh M., Hotta H., Homma M. 1994; A protease activation mutant, MVCES1, as a safe and potent live vaccine derived from currently prevailing Sendai virus. Journal of Virology 68:3369–3373
    [Google Scholar]
  40. Zurcher C., Burek J. D., Van Nunen M. C. J., Meihuizen S. P. 1977; A naturally occurring epizootic caused by Sendai virus in breeding and aging colonies. I. Infection in the mouse. Laboratory Animal Science 27:955–962
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-12-3207
Loading
/content/journal/jgv/10.1099/0022-1317-78-12-3207
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error