The complete sequence of the 5834 nucleotides of RNA 1 of beet soil-borne furovirus (BSBV, Ahlum isolate) was determined using a PCR product obtained with primers to highly conserved coding regions for helicase-like proteins in RNA 1 of furo-, hordei- and tobraviruses as a starting sequence. Unknown parts of the sequence upstream and downstream of this starting sequence were amplified by means of RT-PCR techniques using combinations of specific and random primers. BSBV RNA 1 contains one large ORF for a readthrough protein with a molecular mass of 204 kDa (204K protein) which is interrupted internally by a UAA stop codon terminating the coding region for a protein of 145 kDa (145K protein). The N- and C-terminal parts of the 145K protein and the readthrough domain of the 204K protein contain methyl- transferase, helicase and RNA-dependent RNA polymerase motifs, respectively. Unlike other furo- and tobraviruses BSBV contains no further genes on its RNA 1.
GustafsonG., ArmourS. L., GamboaG. C., BurgettS. G., ShepherdJ. W.1989; Nucleotide sequence of barley stripe mosaic virus RNA α: RNA α encodes a single polypeptide with homology to corresponding proteins from other viruses. Virology 170:370–377
HamiltonW. D. O., BoccaraM., RobinsonD. J., BaulcombeD. C.1987; The complete nucleotide sequence of tobacco rattle virus RNA- 1. Journal of General Virology 68:2563–2575
HerzogE., GuilleyH., ManoharS. K., DolletM., RichardsK., FritschC., JonardG.1994; Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. Journal of General Virology 75:3147–3155
KoenigR., BeierC., CommandeurU., LuthU., KaufmannA., LüddeckeP.1996; Beet soil-borne virus RNA 3 - a further example for the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying RNAs. Virology 216:202–207
KoenigR., CommandeurU., LossS., BeierC., KaufmannA., LesemannD.-E.1997; Beet soil-borne virus RNA 2: similarities and dissimilarities to the coat protein gene-carrying RNAs of other furoviruses. Journal of General Virology 78:469–477
KooninE. V., DoljaV. V.1993; Evolution and taxonomy of positive-strand RNA viruses : implications of comparative analysis of amino acid sequences. Critical Reviews in Biochemistry and Molecular Biology 28:375–430
ScottK. P., KashiwazakiS., ReavyB., HarrisonB. D.1994; The nucleotide sequence of potato mop-top virus RNA 2: a novel type of genome organization for a furovirus. Journal of General Virology 75:3561–3568
ShirakoY., WilsonT. M. A.1993; Complete nucleotide sequence and organization of the bipartite RNA genome of soil-borne wheat mosaic virus. Virology 195:16–32