1887

Abstract

To study the morphogenesis and packaging of rice dwarf phytoreovirus (RDV), the interactions among multiple structural proteins were analysed using both the yeast two-hybrid system and far-Western blotting analysis. The following protein-protein interactions were observed. P3 (major core protein) bound to itself as well as to P7 (nucleic acid-binding protein) and P8 (major outer capsid protein). P7 bound to P1 (RNA-dependent RNA polymerase) and P8, in addition to P3. Based on these findings, we hypothesize that the core shell structure is based on P3-P3 interactions and that P7 has the ability to bind to multiple structural proteins as well as to genomic RNAs during viral particle assembly. Based on the observed protein-protein interactions and on computer-aided analysis of the numbers of structural proteins per particle, possible RDV assembly events are proposed.

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/content/journal/jgv/10.1099/0022-1317-78-12-3135
1997-12-01
2024-12-06
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References

  1. Anzola J. V., Xu Z., Asamizu T., Nuss D. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences USA: 848301–8305
    [Google Scholar]
  2. Inoue H., Timmins P. A. 1985; The structure of rice dwarf virus determined by small-angle neutron scattering measurements. Virology 147:214–216
    [Google Scholar]
  3. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  4. Miyao T., Yasui K., Sakurai H., Yamagishi M., Ishihama A. 1996; Molecular assembly of RNA polymerase from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5. Genes to Cells 1:843–854
    [Google Scholar]
  5. Murao K., Suda N., Uyeda I., Isogai M., Suga H., Yamada N., Kimura I., Shikata E. 1994; Genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of variants of genome segment 12. Journal of General Virology 75:1843–1848
    [Google Scholar]
  6. Nakashima K., Kakutani T., Minobe Y. 1990; Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome. Journal of General Virology 71:725–729
    [Google Scholar]
  7. Nibert M. L., Schiff L. A., Fields B. N. 1996; Reoviruses and their replication. In Fields Virology, 3rd edn. pp 1557–1596 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  8. Omura T., Ishikawa K., Hirano H., Ugaki M., Minobe Y., Tsuchizaki T., Kato H. 1989; The outer capsid protein of rice dwarf virus is encoded by genome segment S8. Journal of General Virology 70:2759–2764
    [Google Scholar]
  9. Patton J. T. 1995; Structure and function of the rotavirus RNA- binding proteins. Journal of General Virology 76:2633–2644
    [Google Scholar]
  10. Rose M. D., Winston F., Hieter P. 1990 Methods in Yeast Genetics :A Laboratory Course Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Roy P. 1996; Orbivirus structure and assembly. Virology 216:1–11
    [Google Scholar]
  12. Suzuki N., Tanimura M., Watanabe Y., Kusano T., Kitagawa Y., Suda N., Kudo H., Uyeda I., Shikata E. 1992; Molecular analysis of rice dwarf phytoreovirus segment S1: interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal- infecting reovirus. Virology 190:240–247
    [Google Scholar]
  13. Suzuki N., Sugawara M., Kusano T., Mori H., Matsuura Y. 1994; Immunodetection of rice dwarf phytoreoviral proteins in both insect and plant hosts. Virology 202:41–48
    [Google Scholar]
  14. Suzuki N., Kusano T., Matsuura Y., Omura T. 1996; Novel NTP binding property of rice dwarf phytoreovirus minor core protein P5. Virology 219:471–474
    [Google Scholar]
  15. Ueda S., Uyeda I. 1997; The rice dwarf phytoreovirus structural protein P7 possesses non-specific nucleic acids binding activity in vitro. Molecular Plant Pathology On-Line [ http://www.bspp.org.uk/mppol1997/0123uyeda
    [Google Scholar]
  16. Uyeda I., Shikata E. 1982; Ultrastructure of rice dwarf virus. Annals of the Phytopathological Society of Japan 48:295–300
    [Google Scholar]
  17. Yamada N., Uyeda I., Kudo H., Shikata E. 1990; Nucleotide sequence of rice dwarf virus segment 3. Nucleic Acids Research 18:6419
    [Google Scholar]
  18. Ye Z. S., Baltimore D. 1994; Binding of Vav to Gab2 through dimerization of Src homology 3 domains. Proceedings of the National Academy of Sciences USA: 9112629–12633
    [Google Scholar]
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