Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins
The genome of Heliothis armigera entomopoxvirus (HaEPV) has been mapped with four restriction endonuclease enzymes (BamHI, HindIII, PstI and XhoI), and its length estimated at 233 kbp. An EcoRI- generated HaEPV genomic fragment hybridized to all fragments identified as genomic termini, providing the first experimental evidence for the presence of terminal repeat elements in an EPV genome. The HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I (NPHI) genes have been cloned and sequenced, and their deduced products shown to possess high levels of identity with homologues from other Genus B entomopoxviruses (EPVs). The genomic locations of these and other HaEPV genes and open reading frames have been determined; comparison of their locations with those of homologues in the Amsacta moorei EPV genome largely supports an hypothesis that the Genus B EPVs share a conserved genomic organization which differs from that of chordopoxviruses. It is proposed that genes of EPVs can be assigned to five actual or predicted homology-based groups, a categorization which is useful for directing and interpreting investigations of EPV gene functions and relationships.
AdamsJ. R., McClintockJ. T.1991; Nuclear polyhedrosis viruses of insects. In Atlas of Invertebrate Viruses pp 87–204AdamsJ. R., BonamiJ. R. Edited by Boca Raton: CRC Press;
Alaoui-IsmaliM. H., RichardsonC. D.1996; Identification and characterisation of a filament-associated protein encoded by Amsacta moorei entomopoxvirus. Journal of Virology 70:2697–2705
BaroudyB. M., VenkatessanS., MossB.1982; Incomplete base- paired flip-flop terminal loops link the two DNA strands of the vaccinia virus genome into one uninterrupted polynucleotide chain. Cell 28:315–324
BinnsM. M., BrittonB. S., MasonC., BoursnellM. E. G.1990; Analysis of the fowlpox genome region corresponding to the vaccinia virus D6 to A1 region: location of, and variation in non-essential genes in poxviruses. Journal of General Virology 71:2873–2881
BroylesS. S., MossB.1987; Identification of the vaccinia virus gene encoding nucleoside triphosphate phosphohydrolase I, a DNA- dependent ATPase. Journal of Virology 61:1738–1742
DallD. J., SriskanthaA., VeraA. P., Lai-FookJ., SymondsT. M.1993; A gene encoding a highly expressed spindle body protein of Heliothis armigera entomopoxvirus. Journal of General Virology 74:1811–1818
GershonP. D., AnsellD. M., BlackD. N.1989; A comparison of the genome organisation of capripoxvirus with that of the orthopoxviruses. Journal of Virology 63:4703–4708
GoodwinR. H., MilnerR. J., BeatonC. D.1991; Entomopox- virinae. In Atlas of Invertebrate Viruses pp 393–434AdamsJ. R., BonamiJ. R. Edited by Boca Raton: CRC Press;
GruidlM. E., HallR. L., MoyerR. W.1992; Mapping and molecular characterization of a functional thymidine kinase from Amsacta moorei entomopoxvirus. Virology 186:507–516
HallR. L., HinkW. F.1990; Physical mapping and field inversion gel electrophoresis of Amsacta moorei entomopoxvirus DNA. Archives of Virology 110:77–90
HallR. L., MoyerR. W.1991; Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames. Journal of Virology 65:6516–6527
HallR. L., MoyerR. W.1993; Identification of an Amsacta spheroidin-like protein within the occlusion bodies of Choristoneura entomopoxviruses. Virology 192:179–187
HuF., SmithC. A., PickupD. J.1994; Cowpox virus contains two copies of an early gene encoding a soluble secreted form of the Type II TNF receptor. Virology 204:343–356
KotwalG. J., MossB.1988a; Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant. Virology 167:524–537
LiX., BarrettJ. W., YuenL., ArifB. M.1997; Cloning, sequencing and transcriptional analysis of the Choristoneura fumiferana entomopoxvirus spheroidin gene. Virus Research 47:143–154
LyttleD. J., FraserK. M., FlemingS. B., MercerA. A., RobinsonA. J.1994; Homologs of vascular endothelial growth factor are encoded by the poxvirus orf virus. Journal of Virology 68:84–92
MassungR. F., JayaramaV., MoyerR. W.1993; DNA sequence analysis of conserved and unique regions of swinepox virus: identification of genetic elements supporting phenotypic observations including a novel G protein receptor-coupled homologue. Virology 197:511–528
MassungR. F., LiuL. I., QiJ., KnightJ. C., YuranT. E., KerlavageA. R., ParsonsJ. M., VenterJ. C., EspositoJ. J.1994; Analysis of the complete genome of smallpox variola major virus strain Bangladesh- 1975. Virology 201:215–240
MockettB., BinnsM. M., BoursnellM. E. G., SkinnerM. A.1992; Comparisons of the locations of homologous fowlpox and vaccinia virus genes reveals major genome reorganization. Journal of General Virology 73:2661–2668
MurphyF. A., FauquetC. M., BishopD. H. L., GhabrialS. A., JarvisA. W., MartelliG. P., MayoM. A., SummersM. D.editors1995Virus Taxonomy. Sixth Report ofthe International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
OsborneR. J., SymondsT. M., SriskanthaA., Lai-FookJ., FernonC. A., DallD. J.1996; An entomopoxvirus homologue of the vaccinia virus D13L-encoded ‘rifampicin resistance’ protein. Journal of General Virology 77:839–846
SanzP., VeyrunesJ. C., CousseransF., BergoinM.1994; Cloning and sequencing of the spherulin gene, the occlusion body major polypeptide of the Melolontha melolontha entomopoxvirus (MmEPV). Virology 202:449–457
SenkevichT. G., KooninE. V., BullerR. M. L.1994; A poxvirus protein with a RING zinc finger motif is of crucial importance for virulence. Virology 198:118–128
SenkevichT. G., WolffeE. J., BullerR. M. L.1995; Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages. Journal of Virology 69:4103–4111
SenkevichT. G., BugertJ. J., SislerJ. R., KooninE. V., DaraiG., MossB.1996; Genome sequence of a human tumorigenic poxvirus : prediction of specific host response-evasion genes. Science 273:813–816
StrayerD. S., JerngH. H., O’ConnorK.1991; Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes. Virology 185:585–595
TomleyF., BinnsM., CampbellJ., BoursnellM.1988; Sequence analysis of an 11·2 kilobase, near-terminal, BamHI fragment of fowlpox virus. Journal of General Virology 69:1025–1040
UptonC., SchiffL., RiceS. A., DowdeswellT., YangX., McFaddenG.1994; A poxvirus protein with a RING finger motif binds zinc and localizes in virus factories. Journal of Virology 68:4186–4195
WalkerJ. E., SarasteM., RunswickM. J., GayN. J.1982; Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO Journal 1:945–951
YuenL., NoiseuxM., GomesM.1991; DNA sequence of the nucleoside triphosphate phosphohydrolase I (NPH I) of the Choristoneura biennis entomopoxvirus. Virology 182:403–406
Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins