1887

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Volume 78, Issue 11

Cloning and functional characterization of the origin of lytic-phase DNA replication of rat cytomegalovirus Free

Abstract

A -acting sequence within the rat cytomegalovirus (RCMV) genome (or/Lyt) that directs initiation of lytic-phase DNA replication is identified in this report. RCMV /Lyt was localized within a 4 3 kb I fragment that is situated immediately upstream of the gene encoding the major DNA-binding protein. The activity of /Lyt was investigated in a transient replication assay, in which the ability of plasmid constructs to promote DNA replication was tested. Replication of Lyt-containing plasmids was autonomous and resulted in the generation of high-molecular-mass concatemers of head-to-tail- linked plasmid oligomers. Lyt-mediated replication was found to depend on viral DNA polymerase activity supplied by RCMV infection. The sequence required for /Lyt function was found to reside within a 3·3 kb II-I fragment. The RCMV or/Lyt sequence is highly complex, containing 23 direct repeats (DRs) and 16 inverted repeats (IRs) of lengths greater than 10 bp. Two of the DRs (DR21 and DR22) are exceptionally large, being 80 and 88 bp in length, respectively. In addition, two sequence elements (of 127 and 120 bp) with dyad symmetry were identified within Lyt. Although the sequence similarity of RCMV /Lyt with its human cytomegalovirus counterpart is limited, there is a striking resemblance in the overall organization of several IRs and DRs within both sequences.

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© Society for General Microbiology 1997
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1997-11-01
2024-04-25
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References

  1. Anders D. G., Punturieri S. M. 1991; Multicomponent origin of cytomegalovirus lytic-phase DNA replication. Journal of Virology 65:931–937
     [Google Scholar]
  2. Anders D. G., Kacica M. A., Pari G., Punturieri S. M. 1992; Boundaries and structure of human cytomegalovirus ori Lyt, a complex origin forlytic-phase DNAreplication. Journal of Virology 66:3373–3384
     [Google Scholar]
  3. Beuken E., Slobbe R., Bruggeman C. A., Vink C. 1996; Cloning and sequence analysis of the genes encoding DNA polymerase, glycoprotein B, ICP18.5 and major DNA-binding protein of rat cytomegalovirus. Journal of General Virology 77:1559–1562
     [Google Scholar]
  4. Briggs M. R., Kadonaga J. T., Bell S. P., Tjian R. 1986; Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science 234:47–52
     [Google Scholar]
  5. Bruggeman C. A., Debie W. H. M., Grauls G., Majoor G., van Boven C. P. A. 1983; Infection of laboratory rats with a new cytomegalo-like virus. Archives of Virology 76:189–199
     [Google Scholar]
  6. Carthew R. W., Chodosh L. A., Sharp P. A. 1985; An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell 43:439–448
     [Google Scholar]
  7. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A.III Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
     [Google Scholar]
  8. Hammerschmidt W., Sugden B. 1988; Identification and characterization of ori Lyt, a lytic origin of DNA replication of EpsteinBarr virus. Cell 55:427–433
     [Google Scholar]
  9. Hamzeh F. M., Lietman P. S., Gibson W., Hayward G. S. 1990; Identification of the lytic origin of DNA replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination. Journal of Virology 64:6184–6195
     [Google Scholar]
  10. Huang L., Zhu Y., Anders D. G. 1996; The variable 3′ ends of a human cytomegalovirus ori Lyt transcript (SRT) overlap an essential, conserved replicator element. Journal of Virology 70:5272–5281
     [Google Scholar]
  11. Kupershmidt S., DeMarchi J. M., Lu Z. Q., Ben-Porat T. 1991; Analysis of an origin of DNA replication located at the L terminus of the genome of pseudorabies virus. Journal of Virology 65:6283–6291
     [Google Scholar]
  12. LaFemina R. L., Hayward G. S. 1983; Replicative forms of human cytomegalovirus DNA with joined termini are found in permissively infected human cells but not in non-permissive Balb/c-3T3 mouse cells. Journal of General Virology 64:373–389
     [Google Scholar]
  13. Lee K. A., Hai T. Y., SivaRaman L., Thimmappaya B., Hurst H. C., Jones N. C., Green M. R. 1987; A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters. Proceedings of the National Academy of Sciences, USA 84:8355–8359
     [Google Scholar]
  14. McVoy M. A., Adler S. P. 1994; Human cytomegalovirus DNA replicates after early circularization by concatemer formation, and inversion occurs within the concatemer. Journal of Virology 68:1040–1051
     [Google Scholar]
  15. Masse M. J. O., Karlin S., Schachtel G. A., Mocarski E. S. 1992; Human cytomegalovirus origin of DNA replication (oriLyt) resides within a highly complex repetitive region. Proceedings of the National Academy of Sciences, USA 89:5246–5250
     [Google Scholar]
  16. Masse M. J. O., Messerle M., Mocarski E. S. 1997; The location and sequence composition of the murine cytomegalovirus replicator (oriLyt). Virology 230:350–360
     [Google Scholar]
  17. Meijer H., Dormans P. H. J., Geelen J.L.M.C., van Boven C. P. A. 1984; Rat cytomegalovirus: studies on the viral genome and the proteins of virions and nucleocapsids. Journal of General Virology 65:681–695
     [Google Scholar]
  18. Meijer H., Dreesen J. C. F. M., van Boven C. P. A. 1986; Molecular cloning and restriction endonuclease mapping of the rat cytomegalovirus genome. Journal of General Virology 67:1327–1342
     [Google Scholar]
  19. Montminy M. R., Bilezikjian L. M. 1987; Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328:175–178
     [Google Scholar]
  20. Sawadago M., Roeder R. G. 1985; Interaction of a gene specific transcription factor with the adenovirus major late promoter upstream of the TATA box region. Cell 43:165–175
     [Google Scholar]
  21. Schepers A., Pich D., Mankertz J., Hammerschmidt W. 1993; cis- acting elements in the lytic origin of DNA replication of Epstein-Barr virus. Journal of Virology 67:4237–4245
     [Google Scholar]
  22. Stow N. D., McMonagle E. C. 1983; Characterization ofthe TRS/IRS origin of DNA replication of herpes simplex virus type 1. Virology 130:427–438
     [Google Scholar]
  23. Vink C., Beuken E., Bruggeman C. A. 1996; Structure of the rat cytomegalovirus genome termini. Journal of Virology 70:5221–5229
     [Google Scholar]
  24. Watanabe S., Yamaguchi N. 1993; Deletion analysis ofa replication origin of human cytomegalovirus by a novel assay system with a combination of microinjection and polymerase chain reaction. Virology 192:332–335
     [Google Scholar]
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Cloning and functional characterization of the origin of lytic-phase DNA replication of rat cytomegalovirus
J. Gen. Virol. 78, 2963 (1997); https://doi.org/10.1099/0022-1317-78-11-2963
/content/journal/jgv/10.1099/0022-1317-78-11-2963
/content/journal/jgv/10.1099/0022-1317-78-11-2963
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