1887

Abstract

Infectious laryngotracheitis virus (ILTV) is an alpha- herpesvirus that causes severe upper respiratory infections in chickens. Although ten putative ILTV glycoprotein genes have been identified by sequence analysis, no ILTV glycoprotein has been extensively characterized. In order to delineate the synthesis and processing pathway of ILTV glycoprotein B (gB), rabbit polyclonal antibodies were raised against a Cro-gB- -galactosidase fusion protein. Through immunoprecipitation analysis of ILTV-infected chicken embryo liver cells it was determined that ILTV gB is initially synthesized as a 110 kDa monomeric precursor protein which rapidly assembles into homodimers composed of 100 kDa subunits. The dimer form of ILTV gB is rapidly cleaved to form two disulphide-linked species of 58 kDa. The apparent reduction in mass (from 110 to 100 kDa) of the mature form of gB during processing in the Golgi apparatus appears to be a common feature of avian herpesvirus gB proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-11-2945
1997-11-01
2022-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/11/9367382.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-11-2945&mimeType=html&fmt=ahah

References

  1. Bagust T. J., Johnson M. A. 1995; Avian infectious laryngotracheitis: virus-host interactions in relation to prospects for eradication. Avian Pathology 24:373–391
    [Google Scholar]
  2. Breshnahan P. A., Hayflick J. S., Molloy S. S., Thomas G. 1993; Endoproteolysis of growth factors and other nonendocrine precursor proteins. In Mechanisms ofIntracellular Trafficking and Processing ofProteins pp. 225–250 Loh P. Y. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  3. Cantin E. M., Eberle R., Baldick J. L., Moss B., Willey D. E., Notkins A. L., Openshaw H. 1987; Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus 1 infection. Proceedings of the National Academy of Sciences, USA 84:5908–5912
    [Google Scholar]
  4. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with the water soluble fluor sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  5. Claesson-Welsh L., Spear P. G. 1986; Oligomerization of herpes simplex virus glycoprotein B. Journal of Virology 60:803–806
    [Google Scholar]
  6. Cover M. S. 1996; The early history of infectious laryngotracheitis. Avian Diseases 40:494–500
    [Google Scholar]
  7. Doms R. W., Lamb R. A., Rose J. K., Helenius A. 1993; Folding and assembly of membrane proteins. Virology 193:545–562
    [Google Scholar]
  8. Fuchs W., Mettenleiter T. C. 1996; DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus. Journal of General Virology 77:2221–2229
    [Google Scholar]
  9. Gainer H. 1993; Intracellular protein trafficking and proprotein processing: an overview. In Mechanisms of Intracellular Trafficking and Processing of Proteins pp. 1–17 Loh P. Y. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  10. Griffin A. M. 1991; Nucleotide sequence of the glycoprotein B gene of infectious laryngotracheitis virus : analysis and evolutionary relationship to the homologous gene from other herpesviruses. Journal of General Virology 72:393–398
    [Google Scholar]
  11. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K. O., Kaplan A. S. 1984; Characterization of the envelope proteins of pseudorabies virus. Journal of Virology 52:583–590
    [Google Scholar]
  12. Highlander S. L., Goins W. F., Person S., Holland T. C., Levine M., Glorioso J. C. 1991; Olig omer formation of the gB glycoprotein of herpes simplex virus type 1. Journal of Virology 65:4275–4283
    [Google Scholar]
  13. Johnson M. A., Prideaux C. T., Kongsuwan K., Sheppard M., Fahey K. J. 1991; Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain. Archives of Virology 119:181–198
    [Google Scholar]
  14. Johnson M. A., Prideaux C. T., Kongsuwan K., Tyack S. G., Sheppard M. 1995a; ICP27 immediate early gene, glycoprotein K (gK) and DNA helicase homologues of infectious laryngotracheitis virus (gallid herpesvirus 1) SA-2 strain. Archives of Virology 140:623–634
    [Google Scholar]
  15. Johnson M. A., Tyack S. G., Prideaux C. T., Kongsuwan K., Sheppard M. 1995b; Sequence characteristics of a gene in infectious laryngotracheitis virus homologous to glycoprotein D of herpes simplex virus. Journal of Sequencing and Mapping 5:191–194
    [Google Scholar]
  16. Keeler C. L. Jr Hazel J. W., Hastings J. E., Rosenberger J. K. 1993; Restriction endonuclease analysis of Delmarva field isolates of infectious laryngotracheitis virus. Avian Diseases 37:418–426
    [Google Scholar]
  17. Keller P. M., Neff B. J., Ellis R. W. 1984; Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes. Journal of Virology 52:293–297
    [Google Scholar]
  18. Kingsley D. H., Hazel J. W., Keeler C. L. Jr 1994; Identification of the infectious laryngotracheitis virus glycoprotein C gene. Virology 203:336–343
    [Google Scholar]
  19. Kongsuwan K., Prideaux C. T., Sheppard M., Fahey M. 1991; Nucleotide sequence of the gene encoding infectious laryngotracheitis virus glycoprotein B. Virology 184:404–410
    [Google Scholar]
  20. Kongsuwan K., Johnson M. A., Prideaux C. T., Sheppard C. T. 1993; Identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted Mr of 32 kilodaltons. Virus Research 29:125–140
    [Google Scholar]
  21. Kongsuwan K., Prideaux C. T., Johnson M. A., Sheppard M., Rhodes S. 1995; Nucleotide sequence analysis of an infectious laryngotracheitis virus gene corresponding to the US3 of HSV-1 and a unique gene encoding a 67 kda protein. Archives of Virology 140:27–39
    [Google Scholar]
  22. Laquerre S., Person S., Glorioso J. C. 1996; Glycoprotein B of herpes simplex virus type 1 oligomerizes through the intermolecular interaction ofa28-amino-aciddomain. JournalofVirology 70:1640–1650
    [Google Scholar]
  23. Loh L. 1991; Synthesis and processing of the major envelope glycoprotein of murine cytomegalovirus. Virology 180:239–250
    [Google Scholar]
  24. Montalvo E. A., Grose C. 1987; Assembly and processing of the disulfide-linked varicella-zoster virus glycoprotein gpII(140). Journal of Virology 61:2877–2884
    [Google Scholar]
  25. Nazerian K., Lee L. F., Yanagida N., Ogawa R. 1992; Protection against Marek’s disease by a fowlpox virus recombinant expressing the glycoprotein B of Marek’s disease virus. Journal of Virology 66:1409–1413
    [Google Scholar]
  26. Niikura M., Matsuura Y., Endoh D., Onuma M., Mikami T. 1992; Expression of the Marek’s disease virus (MDV) homolog of glycoprotein B of herpes simplex virus by recombinant baculovirus and its identification as the B antigen (gp100, gp60, gp49) of MDV. Journal of Virology 60:2631–2638
    [Google Scholar]
  27. Pereira L. 1994; Function of glycoprotein B homologs of the family herpesviridae. Infectious Agents and Disease 3:9–28
    [Google Scholar]
  28. Poss M. L., Mullins J. I., Hoover E. A. 1989; Post-translational modifications distinguish the envelope glycoprotein of the immunodeficiency disease-induced feline leukemia virus retrovirus. Journal of Virology 63:189–195
    [Google Scholar]
  29. Poulsen D. J., Burton C. R. A., O’Brian J. J., Rabin S. J., Keeler C. L. Jr 1991; Identification of the infectious laryngotracheitis virus glycoprotein gB gene by the polymerase chain reaction. Virus Genes 5:335–347
    [Google Scholar]
  30. Prideaux C. T., Kongsuwan K., Johnson M. A., Sheppard M., Fahey K. J. 1992; Infectious laryngotracheitis virus growth, DNA replication, and protein synthesis. Archives of Virology 123:181–192
    [Google Scholar]
  31. Robbins A. K., Weis H. J., Enquist L. W., Watson R. J. 1984; Construction of E. coli expression plasmid libraries : localization of a pseudorabies virus glycoprotein gene. Journal of Molecular and Applied Genetics 2:485–496
    [Google Scholar]
  32. Robbins A. K., Watson R. J., Whealy M. E., Hays W. W., Enquist L. W. 1986; Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex type 1 and type 2 glycoprotein C. Journal of Virology 58:339–347
    [Google Scholar]
  33. Ryan J. P., Whealy M. E., Robbins A. K., Enquist L. W. 1987; Analysis of pseudorabies virus glycoprotein gIII localization and modification by using novel infectious viral mutants carrying unique PcoRI sites. Journal of Virology 61:2962–2972
    [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Sarmiento M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. IV. Conformation of the virion glycoprotein designated VP7(B2). Journal of Virology 29:1159–1167
    [Google Scholar]
  36. Spear P. G. 1985; Glycoproteins specified by herpes simplex viruses. In The Herpesviruses pp. 315–356 Roizman B., Lopez C. Edited by New York: Plenum Press;
    [Google Scholar]
  37. Spear P. G. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology 4:167–180
    [Google Scholar]
  38. Wenske E. A., Bratton M. W., Courtney R. J. 1982; Endo-β-N acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC. Journal of Virology 44:241–248
    [Google Scholar]
  39. Whealy A. E., Robbins A. K., Enquist L. W. 1990; The export pathway of the pseudorabies virus gB homolog gII involves oligomer formation in the endoplasmic reticulum and protease processing in the Golgi apparatus. Journal of Virology 64:1946–1955
    [Google Scholar]
  40. White J. M. 1990; Viral and cellular membrane fusion proteins. Annual Review of Physiology 52:675–697
    [Google Scholar]
  41. Wild M. A., Cook S., Cochran M. 1996; A genomic map of infectious laryngotracheitis virus and the sequence and organization of genes present in the unique short and flanking regions. Virus Genes 12:107–116
    [Google Scholar]
  42. York J. J., Sonza S., Fahey K. J. 1987; Immunogenic glycoproteins of infectious laryngotracheitis herpesvirus. Virology 161:340–347
    [Google Scholar]
  43. Yoshida S., Lee L. F., Yanagida N., Nazarian K. 1994; The glycoprotein B genes of Marek’s disease virus serotypes 2 and 3: identification and expression by recombinant fowl pox viruses. Virology 200:484–493
    [Google Scholar]
  44. Zarling J. M., Moran P. A., Burke R. L., Pachl C., Berman P. W., Lasky L. A. 1986; Human cytotoxic T cell clones directed against herpes simplex virus-infected cells. IV. Recognition and activation by cloned glycoproteins gB and gD. Journal ofImmunology 136:4669–4673
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-11-2945
Loading
/content/journal/jgv/10.1099/0022-1317-78-11-2945
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error