1887

Abstract

Although herpes simplex virus type 1 (HSV-1) does not induce apoptosis in infected HEp-2 cells, in the presence of cycloheximide infection induced apoptosis with characteristic morphological changes as well as endonucleosomal DNA cleavage. The induction of apoptosis without protein synthesis suggests that a structural protein of the HSV-1 virion is responsible for the observed apoptosis.

Apoptosis or programmed cell death (PCD) is a type of animal cell death in which cells die by an active cellular process under the control of a genetically encoded cell suicide programme Apoptosis is characterized morphologically by cell shrinkage, plasma-membrane blebbing, chromatin condensation and nuclear fragmentation and biochemically by degradation of chromosomal DNA into oligonucleosome- sized fragments (Kerr & Harmon, 1991) . Although many animal viruses are known to induce an apoptotic response in infected cells (Clem & Miller, 1994; Shen & Shenk, 1995; White & Gooding, 1994), cells infected with wild-type herpes simplex virus type 1 (HSV-1) do not show apoptotic characteristics . However, based on the finding that mutant virus which lacks the 34.5 gene induces PCD in infected human cells (Chou & Roizman, 1992; Chou . , 1994), the lack of apoptosis in the HSV-1-infected cells has been considered to be an outcome of expression of a viral antiapoptosis gene whose product inhibits one of the steps in the virus-induced signalling leading to PCD . Later, HSV-1 was found to carry, as well as the 34.5 gene, an antiapoptosis gene which can suppress the cellular apoptotic response induced by hyperthermia (Leopardi & Roizman, 1996) or sorbitol treatment (Koyama & Miwa, 1997) .

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1997-11-01
2021-11-29
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References

  1. Chou J., Roizman B. 1992; The γ34.5 gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shut off of protein synthesis characteristics of programmed cell death in neuronal cells. Proceedings of the National Academy of Sciences, USA 893266–3270
    [Google Scholar]
  2. Chou J., Poon A. P. W., Johnson J., Roizman B. 1994; Differential response of human cells to deletions and stop codons in the γ34.5 gene of herpes simplex virus. Journal of Virology 68:8304–8311
    [Google Scholar]
  3. Clem R. J., Miller L. K. 1994; Induction and inhibition of apoptosis by insect viruses. In Apoptosis II; The Molecular Basis of Cell Death pp. 89–110 Tomei L. D., Cope F. O. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. Harris R. A., Preston C. M. 1991; Establishment of latency in vitro by the herpes simplex virus type 1 mutant in 1814. Journal of General Virology 72:907–913
    [Google Scholar]
  5. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell culture. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  6. Jamieson D. R. S., Robinson L. H., Daksis J. I., Nicholl M. J., Preston C. M. 1995; Quiescent viral genomes in human fibroblasts after infection with herpes simplex virus Vmw65 mutants. Journal of General Virology 76:1417–1431
    [Google Scholar]
  7. Kerr J. F. R., Harmon B. V. 1991; Definition and incidence of apoptosis : an historical perspective. In Apoptosis II; The Molecular Basis of Cell Death pp. 5–29 Tomei L. D., Cope F. O. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Koyama A. H. 1995; Induction of apoptotic DNA fragmentation by the infection of vesicular stomatitis virus. Virus Research 37:285–290
    [Google Scholar]
  9. Koyama A. H., Miwa Y. 1997; Suppression of apoptotic DNA fragmentation by the infection of herpes simplex virus. Journal of Virology 71:2567–2571
    [Google Scholar]
  10. Koyama A. H., Uchida T. 1994; Inhibition by brefeldin A of the envelopment of nucleocapsids in herpes simplex virus type 1-infected Vero cells. Archives of Virology 135:305–317
    [Google Scholar]
  11. LaCount D. J., Friesen P. D. 1997; Role of early and late replication events in induction of apoptosis by baculoviruses. Journal of Virology 71:1530–1537
    [Google Scholar]
  12. Leopardi R., Roizman B. 1996; The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by hyperthermia. Proceedings of the National Academy of Sciences, USA 93:9583–9587
    [Google Scholar]
  13. McGarrity G. J. 1979; Detection of contamination. Methods in Enzymology 58:18–29
    [Google Scholar]
  14. Preston C. M., Mabbs R., Nicholl M. J. 1997; Construction and characterization of herpes simplex virus type 1 mutants with conditional defects in immediate early gene expression. Virology 229:228–239
    [Google Scholar]
  15. Roizman B., Sears A. E. 1996; Herpes simplex viruses and their replication. In Fields Virology, 3rd edn. pp. 2231–2295 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  16. Shen Y., Shenk T. E. 1995; Viruses and apoptosis. Current Opinion in Genetics and Development 5:105–111
    [Google Scholar]
  17. White E., Gooding L. R. 1994; Regulat ion of apoptosis by human adenoviruses. In Apoptosis II; The Molecular Basis of Cell Death pp. 111–141 Tomei L. D., Cope F. O. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
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