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Journal of General Virology header logo Journal of General Virology

Volume 78, Issue 11

Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus. No Access

Abstract

The capsid protein of a French isolate of the European brown hare syndrome virus (EBHSV) was expressed in the baculovirus system. The recombinant EBHSV (rEBHSV) capsid protein was able to self-assemble into virus-like particles (VLPs). The VLPs were indistinguishable from the infectious EBHSV and displayed morphological characteristics similar to those we have described for the VLPs resulting from the expression of the capsid protein of rabbit haemorrhagic disease virus (RHDV), a closely related calicivirus. Cross-protection experiments showed that vaccination with rEBHSV particles did not protect rabbits against an RHDV challenge. A set of monoclonal antibodies (MAbs) was raised against rEBHSV capsid protein and used together with anti-RHDV and anti-EBHSV MAbs produced against native viruses to study the antigenic relationships between the two calici- viruses. All six anti-EBHSV MAbs delineated discontinuous epitopes; two of them reacted with specific surface epitopes and the remaining four reacted with internal epitopes which were also present in rRHDV. All anti-RHDV MAbs were monospecific; three reacted with surface linear epitope(s), two reacted with internal linear epitope(s) and one with a surface conformational epitope. On the basis of all these results, a classification of RHDV and EBHSV as two serotypes of a single serogroup is proposed.

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1997-11-01
2025-12-09

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References

  1. Alonso J., Casais R., Boga J. A., Parra F. 1996; Processing of rabbit hemorrhagic disease virus polyprotein. Journal of Virology 70:1261–1265
     [Google Scholar]
  2. Aponte C., Mattion N. M., Estes M. K., Charpilienne A., Cohen J. 1993; Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in the baculovirus system and production of monoclonal antibodies directed against the expressed proteins. Archives of Virology 133:85–95
     [Google Scholar]
  3. Boga J. A., Marin M. S., Casais R., Prieto M., Parra F. 1992; In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV). Virus Research 26:33–40
     [Google Scholar]
  4. Burroughs J. N., Brown F. 1978; Presence of a covalently linked protein on calicivirus RNA. Journal of General Virology 41:443–446
     [Google Scholar]
  5. Capucci L., Scicluna M. T., Lavazza A. 1991; Diagnosis of Viral Haemorrhagic Disease of rabbits and European Brown Hare Syndrome. Revue Scientifique et Technique O.I.E 10:347–370
     [Google Scholar]
  6. Capucci L., Frigoli G., Ronshold L., Lavazza A., Brocchi E., Rossi C. 1995; Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies. Virus Research 37:221–238
     [Google Scholar]
  7. Carter M. J., Milton I. D., Meanger J., Bennett M., Gaskell R. M., Turner P. C. 1992; The complete nucleotide sequence of a feline calicivirus. Virology 190:443–448
     [Google Scholar]
  8. Chasey D., Lucas M., Westcott D., Williams M. 1992; European brown hare syndrome in the U.K.; a calicivirus related to but distinct from that of viral haemorrhagic disease in rabbits. Archives of Virology 124:363–370
     [Google Scholar]
  9. Dingle K. E., Lambden P. R., Caul E. O., Clarke I. N. 1995; Human enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus. Journal of General Virology 76: 2349–2355
     [Google Scholar]
  10. Fuchs A., Weissenböck H. 1992; Comparative histopathological study of Rabbit Haemorrhagic Disease (RHD) and European Brown Hare Syndrome (EBHS). Journal of Comparative Pathology 107:103–113
     [Google Scholar]
  11. Gavier-Widen D., Morner T. 1991; Epidemiology and diagnosis of the European Brown Hare Syndrome in Scandinavian countries : a review. Revue Scientifique et Technique O.I.E 10:453–458
     [Google Scholar]
  12. Gray J. J., Cunlife C., Ball J., Graham D. Y., Desselberger U., Estes M. K. 1994; Detection of immunoglobulin M (IgM), IgA, and IgG Norwalk specific antibodies by indirect enzyme-linked immunosorbent assay with baculovirus-expressed Norwalk virus capsid antigen in adult volunteers challenged with Norwalk virus. Journal of Clinical Microbiology 32:3059–3063
     [Google Scholar]
  13. Green K. Y., Lew J. F., Jiang X., Kapikian A. Z., Estes M. K. 1993; Comparison of the reactivities of baculovirus-expressed recombinant Norwalk virus capsid antigen with those of the native Norwalk virus antigen in serologic assays and some epidemiologic observations. Journal of Clinical Microbiology 31:2185–2191
     [Google Scholar]
  14. Jiang X., Graham D. Y., Wang M., Estes M. K. 1990; Norwalk virus genome cloning and characterization. Science 250:1580–1583
     [Google Scholar]
  15. Jiang X., Wang M., Graham D. Y., Estes M. K. 1992; Expression, self-assembly, and antigenicity of Norwalk virus capsid protein. Journal of Virology 66:6527–6532
     [Google Scholar]
  16. Jiang X., Matson D. O., Ruiz-Palacios G. M., Hu J., Treanor J., Pickering L. K. 1995; Expression, self-assembly, and antigenicity of a snow mountain agent-like calicivirus capsid protein. Journal of Clinical Microbiology 33:1452–1455
     [Google Scholar]
  17. Kapiklian A. Z., Estes M. K., Chanock R. M. 1996; Norwalk group of viruses. In Fields Virology, 3rd edn. pp. 783–810 Fields B. N., Knipe D. M. Edited by Philadelphia: Lipincott-Raven;
     [Google Scholar]
  18. Labbe M., Charpilienne A., Crawford S. E., Estes M. K., Cohen J. 1991; Expression of rotavirus VP2 produces empty corelike particles. Journal of Virology 65:2946–2952
     [Google Scholar]
  19. Lambden P. R., Clarke I. N. 1995; Genome organization in the Caliciviridae. Trends in Microbiology 3:261–265
     [Google Scholar]
  20. Laurent S., Vautherot J.-F., Madelaine M.-F., Le Gall G., Rasschaert D. 1994; Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into virus-like particles and induces protection. Journal of Virology 68:6794–6798
     [Google Scholar]
  21. Le Gall G., Huguet S., Vende P., Vautherot J.-F., Rasschaert D. 1996; European brown hare syndrome virus: molecular cloning and sequencing of the genome. Journal of General Virology 77:1693–1697
     [Google Scholar]
  22. Meyers G., Wirblich C., Thiel H. J. 1991a; Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein- linked and packaged into particles. Virology 184:677–686
     [Google Scholar]
  23. Meyers G., Wirblich C., Thiel H. J. 1991b; Rabbit hemorrhagic disease virus - molecular cloning and nucleotide sequencing of a calicivirus genome. Virology 184:664–676
     [Google Scholar]
  24. Milton I. D., Turner J., Teelan A., Gaskell R., Turner P. C., Carter M. J. 1992; Location of monoclonal antibody binding sites in the capsid protein of feline calicivirus. Journal of General Virology 73:2435–2439
     [Google Scholar]
  25. Monroe S. S., Stine S. E., Jiang X., Estes M. K., Glass R. I. 1993; Detection of antibody to recombinant Norwalk virus antigen in specimens from outbreaks of gastroenteritis. Journal of Clinical Microbiology 31:2866–28 72
     [Google Scholar]
  26. Nagesha H. S., Wang L. F., Hyatt A. D., Morrissy C. J., Lenghaus C., Westbury H. A. 1995; Self-assembly, antigenicity, and immuno- genicity of the rabbit haemorrhagic disease virus (Czechoslovakian strain V-351) capsid protein expressed in baculovirus. Archives of Virology 140:1095–1108
     [Google Scholar]
  27. Neill J. D. 1992; Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among calicivirus capsid proteins. Virus Research 24:211–222
     [Google Scholar]
  28. O’Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors New York: W. H. Freeman;
     [Google Scholar]
  29. Parra F., Prieto M. 1990; Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. Journal of Virology 64:4013–4015
     [Google Scholar]
  30. Prasad B. V., Matson D. O., Smith A. W. 1994a; Three-dimensional structure of calicivirus. Journal of Molecular Biology 240:256–264
     [Google Scholar]
  31. Prasad B. V. V., Rothnagel R., Jiang X., Estes M. K. 1994b; Three-dimensional structure of baculovirus-expressed Norwalk virus capsids. Journal of Virology 68: 5117–5125
     [Google Scholar]
  32. Rasschaert D., Huguet S., Madelaine M.-F., Vautherot J.-F. 1995; Sequence and genomic organization of a rabbit hemorrhagic disease virus isolated from a wild rabbit. Virus Genes 9:121–132
     [Google Scholar]
  33. Sapp M., Volpers C., Müller M., Streeck R. E. 1995; Organization of the major and minor capsid proteins in human papillomavirus type 33 virus-like particles. Journal of General Virology 76: 2407–2412
     [Google Scholar]
  34. Seal B. S., Lutze-Wallace C., Kreutz L. C., Sapp T., Dulac G. C., Neill J. D. 1995; Isolation of caliciviruses from skunks that are antigenically and genotypically related to San Miguel sea lion virus. Virus Research 37:1–12
     [Google Scholar]
  35. Sibilia M., Boniotti M. B., Angoscini P., Capucci L., Rossi C. 1995; Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein. Journal of Virology 69:5812–5815
     [Google Scholar]
  36. Thouvenin E., Laurent S., Madelaine M.-F., Rasschaert D., Vautherot J.-F., Hewat E. A. 1997; Bivalent binding of a neutralising antibody to a calicivirus involves the tortional flexibility of the antibody hinge. Journal of Molecular Biology in press
    [Google Scholar]
  37. Vautherot J.-F., Madelaine M.-F., Boireau P., Laporte J. 1992; Bovine coronavirus peplomer glycoproteins : detailed analyses of S1, S2 and HE. Journal of General Virology 73:1725–1737
     [Google Scholar]
  38. Wirblich C., Meyers G., Ohlinger V. F., Capucci L., Eskens U., Haas B., Thiel H. J. 1994; European brown hare syndrome virus: relationship to rabbit hemorrhagic disease virus and other caliciviruses. Journal of Virology 68:5164–5173
     [Google Scholar]
  39. Wirblich C., Thiel H. J., Meyers G. 1996; Genetic map of the calicivirus rabbit hemorrhagic disease virus as deduced from in vitro translation studies. Journal of Virology 70:7974–7983
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-78-11-2803
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Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus.
J. Gen. Virol. 78, 2803 (1997); https://doi.org/10.1099/0022-1317-78-11-2803
/content/journal/jgv/10.1099/0022-1317-78-11-2803
/content/journal/jgv/10.1099/0022-1317-78-11-2803
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