RT Journal Article SR Electronic(1) A1 Hulst, M M A1 Moormann, R J MYR 1997 T1 Inhibition of pestivirus infection in cell culture by envelope proteins E(rns) and E2 of classical swine fever virus: E(rns) and E2 interact with different receptors. JF Journal of General Virology, VO 78 IS 11 SP 2779 OP 2787 DO https://doi.org/10.1099/0022-1317-78-11-2779 PB Microbiology Society, SN 1465-2099, AB Pure preparations of envelope glycoproteins Erns and E2 of classical swine fever virus (CSFV) synthesized in insect cells were used to study infection of porcine and bovine cells with the pestiviruses CSFV and bovine viral diarrhoea virus (BVDV). Almost 100% inhibition of infection of porcine kidney cells with CSFV was produced by 100 μg/ml Erns. After removal of the virus no Erns was needed in the overlay medium (growth medium) to maintain this level of inhibition. In contrast, 100% inhibition of infection of porcine kidney cells with CSFV by 10 ^g/ml E2 was only achieved when E2 was added to the overlay medium. When E2 was omitted, a maximum of 50% inhibition was achieved. This indicated that after the virus and E2 were removed from the cells, infection still occurred, by virus particles which were still bound to the cell surface. Treatment with 100 μg/ml Erns released these particles from the cell surface. Furthermore, Erns bound irreversibly to the surface of cells susceptible or unsusceptible to pestivirus infection and cell-to-cell spread of CSFV was completely inhibited by E2 but not by Erns. These results demonstrated that Erns and E2 interacted with different cell surface receptors. Inhibition of BVDV infection of porcine and bovine cells by CSFV E2 suggested that CSFV E2 and BVDV E2 share an identical receptor. BVDV strain 5250 isolated from pigs was efficiently inhibited by CSFV Erns, whereas several BVDV strains isolated from cattle were not, suggesting that the conformation of Erns plays a role in host tropism., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-11-2779