1887

Abstract

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific -based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.

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/content/journal/jgv/10.1099/0022-1317-78-11-2747
1997-11-01
2021-10-19
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References

  1. Cabot B., Esteban J. I., Martell M., Genesca J., Vargas V., Esteban R., Guardia J., Gomez J. 1997; Structure of replicating hepatitis C virus (HCV) quasispecies in the liver may not be reflected by analysis of circulating HCV virions. Journal of Virology 71:1732–1734
    [Google Scholar]
  2. Gabrielli A., Manzin A., Candela M., Caniglia M. L., Paolucci S., Danieli M. G., Clementi M. 1994; Active hepatitis C virus infection in bone marrow and peripheral blood mononuclear cells from patients with mixed cryoglobulinaemia. Clinical and Experimental Immunology 97:87–93
    [Google Scholar]
  3. Lanford R. E., Sureau C., Jacob J. R., White R., Fuerst T. R. 1994; .Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 202:606–614
    [Google Scholar]
  4. Lanford R. E., Chavez D., Chisari F. V., Sureau C. 1995; Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specificr Tth reverse transcriptase PCR. JournalofVirology 69: 8079–8083
    [Google Scholar]
  5. Laskus T., Wang L. F., Rakela J., Demetris A. S., Vargas H., Pinna A. D., Tsamandas A. C., Fung J. 1996; Dynamic behavior of hepatitis C virus in chronically infected patients receiving liver graft from infected donors. Virology 220:171–176
    [Google Scholar]
  6. Laskus T., Wang L. F., Radkowski M., Vargas H., Cianciara J., Poutous A., Rakela J. 1997; Comparison of hepatitis B virus core promoter sequences in peripheral blood mononuclear cells and serum from patients with hepatitis B. Journal of General Virology 78: 649–653
    [Google Scholar]
  7. Lerat H., Berby F., Trabaud M.-N., Vidalin O., Major M., Trepo C., Inchauspe G. 1996; Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells. Journal of Clinical Investigation 97:845–851
    [Google Scholar]
  8. Muller H. M., Pfaff E., Goeser T., Kallinowski B., Solbach C., Theilmann L. 1993; Peripheral blood leukocytes serve as a possible extrahepatic site for hepatitis C virus replication. Journal of General Virology 74:669–76
    [Google Scholar]
  9. Saleh M. G., Tibbs C. J., Koskinas J., Pereira L. M. M. B., Bomford A. B., Portmann B. C., McFarlane I. G., Williams R. G. 1994; Hepatic and extrahepatic hepatitis C virus replication in relation to response to interferon therapy. Hepatology 20:1399–1404
    [Google Scholar]
  10. Shimizu Y. K., Purcell R. H., Yoshikura H. 1993; Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro. Proceedings of the National Academy of Sciences, USA 90:6037–6041
    [Google Scholar]
  11. Simmonds P., Holmes E. C., Cha T. A., Chan S. W., McOmish F., Irvine B., Beall E., Yap P. L., Kolberg J., Urdea M. S. 1993; Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS5 region. Journal of General Virology 74:2391–2399
    [Google Scholar]
  12. Wang J. T., Sheu J.-C., Lin J.-T., Wang T.-H., Chen D. S. 1992; Detection of replicative form of hepatitis C virus RNA in peripheral blood mononuclear cells. Journal of Infectious Diseases 166:1167–1169
    [Google Scholar]
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