1887

Abstract

The association between tomato yellow leaf curl geminivirus (TYLCV, Israeli isolate) and its insect vector, the whitefly , was investigated. Insects that emerged during a 24 h period were caged with TYLCV-infected plants for a 48 h acquisition access period, then with egg-plants-a TYLCV non-host-for the rest of their lives. While TYLCV DNA was associated with the whiteflies during their entire adult life, the amount of capsid protein rapidly decreased and was not detectable in the insect after approximately 12 days of age. The ability of the infected whiteflies to transmit TYLCV to tomato test plants steadily decreased with age but did not disappear completely. Transmission by viruliferous insects decreased from 100% to 10–20% during their adult lifetime, compared with a decrease from 100% to 50% for non-viruliferous insects. The association of TYLCV with adult led to a reduction of 17–23% in their life expectancy compared with insects that had not acquired the virus, and to a 40–50% decrease in the mean number of eggs laid. These results suggest that TYLCV has some features reminiscent of an insect pathogen.

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1997-10-01
2024-03-28
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References

  1. Abouzid A. M., Frischmuth T., Jeske H. 1988; A putative replicative form of the Abutilon mosaic virus in chromatin-like structure. Molecular and General Genetics 212:252–258
    [Google Scholar]
  2. Caciagli P., Bosco D., Al-Bitar L. 1995; Relationships of the Sardinian isolate of tomato yellow leaf curl geminivirus with its whitefly vector Bemisia tabaci Gen. European Journal of Plant Pathology 101:163–170
    [Google Scholar]
  3. Cohen S. 1967; The occurrence in the body of Bemisia tabaci of a factor apparently related to the phenomenon of ‘periodic acquisition’ oftomato yellow leaf curl virus. Virology 31:180–183
    [Google Scholar]
  4. Cohen S. 1969; In vivo effects in whiteflies of a possible antiviral factor. Virology 37:448–454
    [Google Scholar]
  5. Cohen S. 1993; Sweet potato whitefly biotypes and their connection with squash silver leaf. Phytoparasitica 21:174
    [Google Scholar]
  6. Cohen S., Antignus Y. 1994; Tomato yellow leaf curl virus, a whitefly-borne geminivirus of tomatoes. Advances in Disease Vector Research 10:259–288
    [Google Scholar]
  7. Cohen S., Harpaz I. 1964; Periodic, rather than continual, acquisition of a new tomato virus by its vector, the tobacco whitefly (Bemisia tabaci Gennadius). Entomologica Experimentalis Applicata 7:155–166
    [Google Scholar]
  8. Cohen S., Nitzany F. E. 1966; Transmission and host range of the tomato yellow leaf curl virus. Phytopathology 56:1127–1131
    [Google Scholar]
  9. Cohen S., Duffus J. E., Berlinger M. J. 1987; Epidemiological studies on whitefly-transmitted viruses in California and Israel. BARD Report ProjectI-583–83
    [Google Scholar]
  10. Coudriet D. L., Prabhaker N., Kishaba A. N., Meyerdirk D. E. 1985; Variation in developmental rate on different hosts and overwintering of the sweetpotato whitefly, Bemisia tabaci (Homoptera: Aleyrodidae). Environmental Entomology 14:516–519
    [Google Scholar]
  11. Czosnek H., Ber R., Antignus Y., Cohen S., Navot N., Zamir D. 1988; Isolation of the tomato yellow leaf curl virus -a geminivirus. Phytopathology 78:508–512
    [Google Scholar]
  12. Czosnek H., Laterrot H. 1997; A worldwide survey of tomato yellow leaf curl viruses. Archives of Virology 142: in press
    [Google Scholar]
  13. Duffus J. E. 1987; Whitefly transmission of plant viruses. In Current Topics in Vector Research 4 pp 73–91 Harris K. Edited by New York: Springer-Verlag;
    [Google Scholar]
  14. Gameel O. I. 1974; Some aspects of the mating and oviposition behaviour of the cotton whitefly Bemisia tabaci (Genn. ) Revue Zoologie Botanie Africaine 88:784–788
    [Google Scholar]
  15. Gerling D., Horowitz A. R., Baumgartner J. 1986; Autecology of Bemisia tabaci . Agriculture Ecosystem and Environment 17:5–19
    [Google Scholar]
  16. Horowitz A. R., Podoler H., Gerling D. 1984; Life table analysis of the tobacco whitefly Bemisia tabaci (Gennadius) in cotton fields in Israel. Acta OecologicajOecologica Applicata 5:221–233
    [Google Scholar]
  17. Kunik T., Salomon R., Zamir D., Navot N., Zeidan M., Michelson I., Gafni Y., Czosnek H. 1994; Transgenic tomato expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus. Bio/Technology 12:500–504
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  19. Mehta P., Wyman J. A., Nakhla M. K., Maxwell D. P. 1994; Transmission of tomato yellow leaf curl geminivirus by Bemisia tabaci (Homoptera: Aleyrodidae). Journal of Economical Entomology 87:1291–1297
    [Google Scholar]
  20. Navot N., Ber R., Czosnek H. 1989; Rapid detection of tomato yellow leaf curl virus in squashes of plant and insect vectors. Phytopathology 79:562–568
    [Google Scholar]
  21. Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H. 1991; Tomato yellow leaf curl virus : a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151–161
    [Google Scholar]
  22. Padidam M., Beachy R. N., Fauquet C. M. 1995; Classification and identification of geminiviruses using sequence comparisons. Journal of General Virology 76:249–263
    [Google Scholar]
  23. Pesic-van Esbroeck Z., Harris K. F., Duffus J. E. 1995; Immuno- cytochemical localization of squash leaf curl virus (SLCV) in squash and the sweet potato whitefly. Phytopathology 85:1180
    [Google Scholar]
  24. Rybicki E. P. 1994; A phylogenetic and evolutionary justification for three genera of Geminiviridae. Archives of Virology 139:49–77
    [Google Scholar]
  25. SAS Institute 1995; JMP Statistics and Graphics Guide. Version 3.1. SAS Institute Inc, Cary, NC; USA:
    [Google Scholar]
  26. Zeidan M., Czosnek H. 1991; Acquisition of tomato yellow leaf curl virus by the whitefly Bemisia tabaci. Journal of General Virology 72:2607–2614
    [Google Scholar]
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