1887

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Volume 78, Issue 10

The gene encoding the capsid protein P82 of the Choristoneura fumiferana multicapsid nucleopolyhedrovirus: sequencing, transcription and characterization by immunoblot analysis Free

Abstract

A gene encoding a capsid-associated viral structural protein has been identified and sequenced in the genome of the multicapsid nucleopolyhedrovirus (CfMNPV). The gene has a 1872 nucleotide open reading frame (ORF) encoding 624 amino acids with a predicted molecular mass of 71·4 kDa. Transcription, which appeared to be initiated from a conserved GTAAG motif of baculovirus late genes, was detected at 12 h, reached a maximum at 48 h and declined at 72 h post-infection (p.i.). Part of the ORF was cloned in frame into a prokaryotic expression vector, pMAL- c2, and the fusion protein was used to generate antibodies in rabbits. It was shown, with the aid of the polyclonal antiserum, that this viral protein was detectable at 24 h p.i. in infected cells. The protein appeared as an 82 kDa band in occlusion-derived virus and as an 82 kDa band and a 72 kDa band in budded virus. Amino acid sequence comparisons revealed that this ORF had high homology with the ORF p87 (77% similarity) of (Op) MNPV and the ORF p80 (60% similarity) of (Ac) MNPV. Immunoblots confirmed that the CfMNPV protein had antigenic similarities to the P87 protein of OpMNPV, but not to the P80 of AcMNPV.

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© Society for General Microbiology 1997
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/content/journal/jgv/10.1099/0022-1317-78-10-2665
1997-10-01
2023-09-24
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The gene encoding the capsid protein P82 of the Choristoneura fumiferana multicapsid nucleopolyhedrovirus: sequencing, transcription and characterization by immunoblot analysis
J. Gen. Virol. 78, 2665 (1997); https://doi.org/10.1099/0022-1317-78-10-2665
/content/journal/jgv/10.1099/0022-1317-78-10-2665
/content/journal/jgv/10.1099/0022-1317-78-10-2665
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