1887

Abstract

A baculovirus ( nucleopoly- hedrovirus) vector containing a strong promoter, the CAG promoter, was developed to introduce foreign genes into mammalian cells. Recombinant baculoviruses carrying a reporter gene under the control of the CAG promoter were inoculated into various mammalian cell lines. High-level expression was observed not only in hepatocytes but also in other non-hepatic cell lines tested. Expression of the reporter gene was detected even 14 days after infection. The infectious titre of the recovered baculoviruses decreased significantly after infection, indicating that the baculoviruses did not replicate in mammalian cells. We then compared the efficiencies of gene expression by the baculovirus vector with that of a replication-defective adenovirus vector by using the same expression unit. The same level of expression was observed in HepG2, HeLa and COS7 cells by both vectors. Efficient expression and proper processing were observed in mammalian cells infected with baculoviruses carrying genes coding for structural regions of hepatitis C virus. These results suggest that the baculovirus vector is a good tool for gene delivery into various mammalian cells in order to study the function of foreign genes.

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/content/journal/jgv/10.1099/0022-1317-78-10-2657
1997-10-01
2022-05-23
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References

  1. Anderson W. F. 1992; Human gene therapy. Science 256:808–813
    [Google Scholar]
  2. Boyce F. M., Bucher N. L. R. 1996; Baculovirus-mediated gene transfer into mammalian cells. Proceedings of the National Academy of Sciences USA: 932348–2352
    [Google Scholar]
  3. Carbonell L. F., Klowden M. J., Miller L. K. 1985; Baculovirus- mediated expression of bacterial genes in dipteran and mammalian cells. Journal of Virology 56:153–160
    [Google Scholar]
  4. De Wet J. R., Wood K. V., DeLuca M., Helinski D. R., Subramani S. 1987; Firefly luciferase gene: structure and expression in mammalian cells. Molecular Cellular Biology 7:725–737
    [Google Scholar]
  5. Fujita A., Sakagami K., Kanegae Y., Saito I., Kobayashi I. 1995; Gene targeting with a replication-defective adenovirus vector. Journal of Virology 69:6180–6190
    [Google Scholar]
  6. Harada S., Watanabe Y., Takeuchi K., Suzuki T., Katayama T., Takebe Y., Saito I., Miyamura T. 1991; Expression of processed core protein of hepatitis C virus in mammalian cells. Journal of Virology 65:3015–3021
    [Google Scholar]
  7. Hofmann C., Sandig V., Jennings G., Rudolph M., Schlag P., Strauss M. 1995; Efficient gene transfer into human hepatocytes by baculovirus vectors. Proceedings of the National Academy of Sciences USA: 9210099–10103
    [Google Scholar]
  8. Kanegae Y., Makimura M., Saito I. 1994; A simple and efficient method for purification of infectious recombinant adenovirus. Japanese Journal of Medical Science & Biology 47:157–166
    [Google Scholar]
  9. Kanegae Y., Lee G., Sato Y., Tanaka M., Nakai M., Sakaki T., Sugano S., Saito I. 1995; Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Research 23:3816–3821
    [Google Scholar]
  10. Kojima H., Inuzuka S., Miwa T., Furukawa S., Hayashi K., Kanegae Y., Saito I., Ohishi N., Takamori M., Yagi K. 1995; Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor. Biochemical and Biophysical Research Communications 212:712–717
    [Google Scholar]
  11. Komaniwa H., Fukusho A., Shimizu Y. 1981; Micro method for performing titration and neutralization test of hog cholera virus using established porcine kidney cell strain. National Institute of Animal Health Quarterly (Japan) 21:153–158
    [Google Scholar]
  12. Kool M., Ahrens C. H., Goldbach R. W., Rohrmann G. F., Vlak J. M. 1994; Identification of genes involved in DNA replication of the Autographa californica baculovirus. Proceedings of the National Academy of Sciences USA: 9111212–11216
    [Google Scholar]
  13. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Biotechnology 6:47–55
    [Google Scholar]
  14. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  15. Matsuura Y., Suzuki T., Suzuki R., Sato M., Aizaki H., Saito I., Miyamura T. 1994; Processing of E1 and E2 glycoproteins ofhepatitis C virus expressed in mammalian and insect cells. Virology 205:141–150
    [Google Scholar]
  16. Mittal S. K., McDermott M. R., Johnson D. C., Prevec L., Graham F. L. 1993; Monitoring foreign gene expression by a human adenovirus-based vector using the firefly luciferase gene as a reporter. Virus Research 28:67–90
    [Google Scholar]
  17. Mulligan R. C. 1993; The basic science of gene therapy. Science 260:926–932
    [Google Scholar]
  18. Negro F., Pacchioni D., Shimizu Y., Miller R. H., Bussolati G., Purcell R. H., Bonino F. 1992; Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology. Proceedings of the National Academy of Sciences USA: 892247–2251
    [Google Scholar]
  19. Nielsen D. A., Chou J., MacKrell A. J., Casadaban M. J., Steiner D. F. 1983; Expression of a preproinsulin-β-galactosidase gene fusion in mammalian cells. Proceedings of the National Academy of Sciences USA: 805198–5202
    [Google Scholar]
  20. Niwa H., Yamamura K., Miyazaki J. 1991; Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–200
    [Google Scholar]
  21. Schöler H. R., Balling R., Hatzopoulos A. K., Suzuki N., Gruss P. 1989; Octamer binding proteins confer transcriptional activity in early mouse embryogenesis. EMBO Journal 8:2551–2557
    [Google Scholar]
  22. Shimizu Y. K., Iwamoto A., Hijikata M., Purcell R. H., Yoshikura H. 1992; Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proceedings of the National Academy of Sciences USA: 895477–5481
    [Google Scholar]
  23. Smith G. E., Summers M. D., Fraser M. J. 1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
    [Google Scholar]
  24. Suzuki R., Matsuura Y., Suzuki T., Ando A., Chiba J., Harada S., Saito I., Miyamura T. 1995; Nuclear localization of the truncated hepatitis C virus core protein with its hydrophobic C terminus deleted. Journal of General Virology 76:53–61
    [Google Scholar]
  25. Takeuchi K., Kubo Y., Boonmar S., Watanabe Y., Katayama T., Choo Q.-L., Kuo G., Houghton M., Saito I., Miyamura T. 1990; The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. Journal of General Virology 71:3027–3033
    [Google Scholar]
  26. Wyatt L. S., Moss B., Rozenblatt S. 1995; Replication -deficient vaccine virus encoding bacteriophage T7 RNA polymerase for transient gene expression in mammalian cells. Virology 210:202–205
    [Google Scholar]
  27. Yang Y., Nunes F. A., Berensci K., Furth E. E., Gönczöl E., Wilson J. M. 1994; Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. Proceedings of the National Academy of Sciences USA: 914407–4411
    [Google Scholar]
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