1887

Abstract

We have demonstrated that COS7 cells transiently co-expressing myristylation-defective (Myr ) and protease-defective (PR ) human immunodeficiency virus (HIV) mutants can release infectious virions when co-transfected with an amphotropic murine leukaemia virus envelope protein expression plasmid (SV-A-MLV-env). In contrast, no infectious virions were detected when a PR, noninfectious HIV mutant was co-expressed with the Myr mutant, although the Myr mutant could still process the immature core particles in . This result indicates that generation of functionally normal Gag proteins is required for virus infectivity in our complementation system. A mutant with a 56- amino-acid deletion in the N-terminal region of the capsid (CA) domain could still complement the PR mutant to generate infectious virions, suggesting that the deletion mutant could provide a functional protease for processing in the PR mutant. This result is consistent with the concept that mutations within the N-terminal region of the CA domain have no major effects on Gag-Pol incorporation into particles.

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1997-10-01
2022-05-28
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