1887

Abstract

A low frequency peripheral blood mononuclear cell (PBMC) subpopulation, referred to as natural interferon-producing (NIP) cells, is described as producing interferon-α (IFN-α) following contact with non-infectious viral structures, namely viral glycoproteins. These cells are characterized as non-T, non-B, MHC class II and CD4 cells. In this study, NIP cells were analysed after an intravenous injection of UV-inactivated transmissible gastroenteritis virus in newborn piglets, which resulted in strong serum IFN-α production. Spleno-cytes, but not PBMC, were the IFN-α producers . Using double immunohistochemical labelling for both IFN-α and leukocyte markers, we established that splenic NIP cells were not T or B cells. The majority were MHC class II and only a minority expressed a macrophage marker. NIP cells were localized in contact with MHC class II-expressing cells and T cells, which suggested that NIP cells might modulate the antiviral immune response.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-10-2483
1997-10-01
2022-05-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/10/9349468.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-10-2483&mimeType=html&fmt=ahah

References

  1. Artursson K., Lindersson M., Varela N., Scheynius A., Alm G. V. 1995; Interferon-a production and tissue localization of interferon-α/β producing cells after intradermal administration of Aujeszky’s disease virus-infected cells in pigs. Scandinavian Journal of Immunology 41:121–129
    [Google Scholar]
  2. Belardelli F., Gresser I. 1996; The neglected role of type I interferon in the T-cell response: implications for its clinical use. Immunology Today 17:369–372
    [Google Scholar]
  3. Bhuiya T. A., Shodell M., Fitzgerald-Bocarsly P. A., Murasko D., Shah K., Drake D., Siegal F. P. 1994; Interferon-α generation in mice responding to challenge with UV-inactivated herpes simplex virus. Journal of Interferon Research 14:17–24
    [Google Scholar]
  4. Cederblad B., Alm G. V. 1990; Infrequent but highly efficient interferon-α producing human mononuclear leukocytes induced by herpes simplex virus in vitro studied by immuno-plaque and limiting dilution assays. Journal of Interferon Research 10:65–73
    [Google Scholar]
  5. Charley B., Laude H. 1988; Induction of alpha interferon by transmissible gastroenteritis coronavirus : role of a transmembrane glycoprotein E1. Journal of Virology 62:8–11
    [Google Scholar]
  6. Diaz de Arce H., Artursson K., L’Haridon R., Perers A., La Bonnardière C., Alm G. V. 1992; A sensitive immunoassay for porcine interferon-a. Veterinary Immunology and Immunopathology 30:319–327
    [Google Scholar]
  7. Eloranta M.-L., Sandberg K., Alm G. V. 1996; The interferon-α/β responses of mice to herpes simplex virus studied at the blood and tissue level in vitro and in vivo. Scandinavian Journal of Immunology 43:355–360
    [Google Scholar]
  8. Ferbas J. J., Toso J. F., Logar A. J., Navratil J. S., Rinaldo C. R.Jr 1994; CD4 + blood dendritic cells are potent producers of IFN-α in response to in vitro HIV-1 infection. Journal of Immunology 152:4649–4662
    [Google Scholar]
  9. Fitzgerald-Bocarsly P. 1993; Human natural interferon-a producing cells. Pharmacology and Therapeutics 60:39–62
    [Google Scholar]
  10. Francis M. L., Meltzer M. S. 1993; Induction of IFN-a by HIV-1 in monocyte-enriched PBMC requires gp120-CD4 interaction but not virus replication. Journal of Immunology 151:2208–2216
    [Google Scholar]
  11. Gobl A. E., Funa K., Alm G. V. 1988; Different induction patterns of mRNA for IFN-α and IFN-β in human mononuclear leukocytes after in vitro stimulation with herpes simplex virus-infected fibroblasts and Sendai virus. Journal of Immunology 140:3605–3609
    [Google Scholar]
  12. Grage-Griebenow E., Flad H.-D., Ernst M. 1996; Fcy receptor I (CD64)-negative human monocytes are potent accessory cells in viral antigen-induced T cell activation and exhibit high IFN-α-producing capacity. Journal of Leukocyte Biology 60:389–396
    [Google Scholar]
  13. Hammerberg C., Schurig G. G. 1986; Characterization of monoclonal antibodies directed against swine leucocytes. Veterinary Immunology and Immunopathology 11:107–121
    [Google Scholar]
  14. Kaeffer B., Bottreau E., Marcon D., Olivier M., Lantier I., Salmon H. 1991; Histocompatible miniature pig (d/d haplotype): generation of hybridomas secreting A or M monoclonal antibody. Hybridoma 10:731–744
    [Google Scholar]
  15. La Bonnardière C., Laude H. 1981; High interferon titer in newborn pig intestine during experimentally induced viral enteritis. Infection and Immunity 32:28–31
    [Google Scholar]
  16. Laude H., Gelfi J., Lavenant L., Charley B. 1992; Single amino acid changes in the viral glycoprotein M affect induction of alpha interferon by the coronavirus transmissible gastroenteritis virus. Journal of Virology 66:743–749
    [Google Scholar]
  17. Lefèvre F., L’Haridon R., Borras-Cuesta F., La Bonnardière C. 1990; Production, purification and biological properties of an Escherichia coli -derived recombinant porcine alpha interferon. Journal of General Virology 71:1057–1063
    [Google Scholar]
  18. Nowacki W., Charley B. 1993; Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: a study with pig leukocytes. Research in Immunology 144:111–120
    [Google Scholar]
  19. Nowacki W., Cederblad B., Renard C., La Bonnardière C., Charley B. 1993; Age -related increase of porcine natural interferon α producing cell frequency and of interferon yield per cell. Veterinary Immunology and Immunopathology 37:113–122
    [Google Scholar]
  20. Pescovitz M. D., Lunney J. K., Sachs D. H. 1984; Preparation and characterization of monoclonal antibodies reactive with porcine PBL. Journal of Immunology 133:368–375
    [Google Scholar]
  21. Riffault S., Eloranta M.-L., Carrat C., Sandberg K., Charley B., Alm G. 1996; Herpes simplex virus induces appearance of interferon-α/β- producing cells and partially interferon-α/β-dependent accumulation of leukocytes in murine regional lymph nodes. Journal of Interferon and Cytokine Research 16:1007–1014
    [Google Scholar]
  22. Riffault S., Grosclaude J., Vayssier M., Laude H., Charley B. 1997; Reconstituted coronavirus TGEV virosomes lose the virus ability to induce porcine interferon-alpha production. Veterinary Research 28:105–114
    [Google Scholar]
  23. Sandberg K., Eloranta M. L., Johannisson A., Alm G. V. 1991; Flow cytometric analysis of natural interferon-α producing cells. Scandinavian Journal of Immunology 34:565–576
    [Google Scholar]
  24. Sandberg K., Eloranta M.-L., Campbell I. L. 1994; Expression of alpha/beta interferons (IFN-α/β) and their relationship to IFN-α/β- induced genes in lymphocytic choriomeningitis. Journal of Virology 68:7358–7366
    [Google Scholar]
  25. Splichal I., Rehakova Z., Sinkora J., Charley B., Sinkora M. 1995; Interferon alpha secreting cells in hematopoietic organs of pig fetuses after in vivo stimulation by coronavirus TGEV. Scandinavian Journal of Immunology 41:642
    [Google Scholar]
  26. Svensson H., Johannisson A., Nikkilä T., Alm G. V., Cederblad B. 1996; The cell surface phenotype of human natural IFN-α producing cells as determined by flow cytometry. Scandinavian Journal of Immunology 44:164–172
    [Google Scholar]
  27. Van den Broek M. F., Müller U., Huang S., Zinkernagel R. M., Aguet M. 1995; Immune defence in mice lacking type I and/or type II interferon receptors. Immunological Reviews 148:5–18
    [Google Scholar]
  28. Yang H., Parkhouse R. M. E. 1996; Phenotyp ic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology 89:76–83
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-10-2483
Loading
/content/journal/jgv/10.1099/0022-1317-78-10-2483
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error