1887

Abstract

Fusion regulatory protein-1 (FRP-1) regulates virus-mediated cell fusion and induces polykaryocyte formation of monocytes without any fusogen. We have recently reported that FRP-1 and the 4F2/CD98 heavy chain are identical molecules. Cell fusion in Newcastle disease virus (NDV)-infected HeLa cells was enhanced when cells were incubated with anti-FRP-1 MAb. Anti-FRP-1 MAbs also induced human immunodeficiency virus gp160-mediated cell fusion. However, HBJ127, an anti-FRP- 1/4F2/CD98 MAb that enhanced cell fusion in NDV- infected cells, delayed human parainfluenza virus type 2 (HPIV-2)-induced cell fusion in HeLa cells, although these viruses belong to the same genus . No anti-FRP-1 MAbs enhanced cellfusion in HPIV-2-infected HeLa cells. Anti-FRP-1 MAbs including HBJ127 showed no effect on virus growth and expression levels of virus-specific polypeptides in HPIV-2-infected HeLa cells, indicating thatthe delay in cell fusion by an anti-FRP-1 MAb is not due to suppression of virus replication. When HeLa cells were transfected with an expression vector harbouring HPIV-2 HN and F genes, cell fusion was also suppressed by HBJ127, but the effect was weak in comparison with virus-infected cells. These data indicate anti-FRP-1 antibodies not only induce/enhance, but also inhibit/delay virus- induced cell fusion and therefore FRP-1 molecules are multifunctional.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-1-83
1997-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/1/9010289.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-1-83&mimeType=html&fmt=ahah

References

  1. Ebata S. N., Cote M. J., Kang C. Y., Dimock K. 1991; The fusion and hemagglutinin-neuraminidase glycoproteins of human parainfluenza type 3 are both required for fusion. Virology 183:437–441
    [Google Scholar]
  2. Hu X., Ray R., Compans R. W. 1992; Functional interactions between the fusion protein and hemagglutinin-neuraminidase of human parainfluenza viruses. Journal of Virology 66:1528–1534
    [Google Scholar]
  3. Ito Y., Tsurudome M., Yamada A., Hishiyama M. 1987; Induction of cell fusion in Newcastle disease virus-infected L929 cells by anti-L929 cell antisera. Journal of General Virology 68:1261–1266
    [Google Scholar]
  4. Ito Y., Komada H., Kusagawa S., Tsurudome M., Matsumura H., Kawano M., Ohta H., Nishio M. 1992; Fusion regulation proteins on the cell surface: isolation and characterization of monoclonal antibodies which enhance giant polykaryocyte formation in Newcastle disease virus-infected cell lines of human origin. Journal of Virology 66:5999–6007
    [Google Scholar]
  5. Kawano M., Bando H., Ohgimoto S., Kondo K., Tsurudome M., Nishio M., Ito Y. 1990a; Sequence of the fusion protein gene of human parainfluenza type 2 virus and its 3ʹ intergenic region: lack of small hydrophobic (SH) gene. Virology 178:289–292
    [Google Scholar]
  6. Kawano M., Bando H., Yuasa Y., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1990b; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174:308–313
    [Google Scholar]
  7. Koga Y., Sasaki M., Yoshida H., Wigzell H., Kimura G., Nomoto K. 1990; Cytopathic effect determined by the amount of CD4 molecules in human cell lines expressing envelope glycoprotein of HIV. Journal of Immunology 144:94–102
    [Google Scholar]
  8. Morrison T., McQuain C., McGinnes L. 1991; Complementation between avirulent Newcastle disease virus and a fusion protein gene expressed from a retrovirus vector: requirement for membrane fusion. Journal of Virology 65:813–822
    [Google Scholar]
  9. Ohgimoto S., Tabata N., Suga S., Nishio M., Ohta H., Tsurudome M., Komada H., Kawano M., Watanabe N., Ito Y. 1995; Molecular characterization of fusion regulatory protein-1 (FRP-1) that induces multinucleated giant cell formation of monocytes and HIV gp160- mediated cell fusion. Journal of Immunology 155:3585–3592
    [Google Scholar]
  10. Ohta H., Tsurudome M., Matsumura H., Koga Y., Morikawa S., Kawano M., Kusagawa S., Komada H., Nishio M., Ito Y. 1994; Molecular and biological characterization of fusion regulatory proteins (FRPs) : anti-FRP mAbs induced HIV-mediated cell fusion via an integrin system. EMBO Journal 13:2044–2055
    [Google Scholar]
  11. Tabata N., Ito M., Shimokata K., Suga S., Ohgimoto S., Tsurudome M., Kawano M., Matsumura H., Komada H., Nishio M., Ito Y. 1994; Expression of fusion regulatory proteins (FRPs) on human peripheral blood monocytes. Journal of Immunology 153:3256–3266
    [Google Scholar]
  12. Takebe Y., Seiki M., Fujisawa J., Hoy P., Yokota K., Arai K., Yoshida M., Arai N. 1988; SRa promotor and the R-U5 segment of the human T-cell leukemia virus type 1 long terminal repeat. Molecular and Cellular Biology 8:466–472
    [Google Scholar]
  13. Teixeira S., Grandi S. D., Kuhn L. C. 1987; Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus. Journal of Biological Chemistry 20:9574–9580
    [Google Scholar]
  14. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses re-vealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
  15. Tsurudome M., Kawano M., Yuasa T., Tabata N., Nishio M., Komada H., Ito Y. 1995; Identification of regions on the hemagglutinin-neuraminidase protein of human parainfluenza virus type 2 important for promoting cell fusion. Virology 213:190–203
    [Google Scholar]
  16. Yuasa T., Kawano M., Tabata N., Nishio M., Kusagawa S., Komada H., Matsumura H., Ito Y., Tsurudome M. 1995; A cell fusion- inhibiting monoclonal antibody binds to the presumed stalk domain of the human parainfluenza type 2 virus hemagglutinin-neuraminidase protein. Virology 206:1117–1125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-1-83
Loading
/content/journal/jgv/10.1099/0022-1317-78-1-83
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error