@article{mbs:/content/journal/jgv/10.1099/0022-1317-78-1-77, author = "Di Bonito, P. and Mochi, S. and Gró, M. C. and Fortini, D and Giorgi, C", title = "Organization of the M genomic segment of Toscana phlebovirus", journal= "Journal of General Virology", year = "1997", volume = "78", number = "1", pages = "77-81", doi = "https://doi.org/10.1099/0022-1317-78-1-77", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-1-77", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The nucleotide sequence of the Toscana (TOS) virus M RNA segment contains a single major open reading frame in the viral-complementary sequence, which can encode a polyprotein of 1339 amino acids. To map the TOS M segment product(s), different regions of the putative M polypeptide were expressed as glutathione S-transferase fusion proteins, which were purified and inoculated into mice to produce hyperimmune sera. By Western blot analysis, a protein of approximately 30 kDa and two glycoproteins, G1 and G2, with the same molecular mass (approximately 65 kDa) were identified in TOS virus-infected cells. The 30 kDa protein, which reacted with antibodies raised to the NH2-terminal, was found to be a non-structural protein (designated NSm). By immunoprecipitation analysis of TOS virus-infected cell lysates, both treated or untreated with tunicamycin, the relative positions of glycoproteins G1 and G2 were determined. The gene order, with respect to the genomic M RNA, was found to be 3′ NSm-G1-G2 5′.", }