1887

Abstract

We have analysed the ability of herpes simplex virus type 2 (HSV-2) to induce nitric oxide (NO) production in resting BALB/c mouse peritoneal macrophages. In most experiments, macrophages produced very small amounts of NO upon infection with HSV-2. Mock virus preparations did not induce NO production, and virus inactivation experiments showed that infectious virus was required. Since interferon-(IFN-) is the prototype cytokine that is able to induce significant NO production in macrophages, we found it of interest to examine the influence of HSV-2 infection on the IFN--induced NO production. The virus exerted a synergistic effect on the IFN--induced NO release, which was accompanied by induction of the as revealed by RT-PCR. This effect was largely dependent on the presence of infectious virus particles, since only a minor effect was seen with mock virus and inactivated virus preparations. From experiments with neutralizing antibodies to tumour necrosis factor-(TNF-) and IFN-/ it was concluded that the synergistic effect is dependent on autocrine secretion of TNF-, which acts as a second signal and synergizes with IFN- in NO production.

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1997-01-01
2022-09-28
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