RT Journal Article SR Electronic(1) A1 Shanks, M. A1 Dessens, J. T. A1 Lomonossoff, G. P.YR 1996 T1 The 24 kDa Proteinases of Comoviruses are Virus-specific in cis as Well as in trans JF Journal of General Virology, VO 77 IS 9 SP 2365 OP 2369 DO https://doi.org/10.1099/0022-1317-77-9-2365 PB Microbiology Society, SN 1465-2099, AB To investigate the specificity of comoviral 24 kDa (‘24K’) proteinases, a full-length cDNA copy of red clover mottle virus (RCMV) RNA 1 has been cloned downstream of a T7 promoter. Translation in rabbit reticulocyte lysates of in vitro transcripts from this clone resulted in the synthesis of a 200K protein which was processed in a manner similar to that of the equivalent protein from cowpea mosaic virus (CPMV). Full-length cDNA clones of the RNA 1 molecules of RCMV and CPMV were used to create hybrid RNA 1 molecules. RNA transcribed in vitro from these hybrids was translated in vitro and the ability of the 24K proteinase from one comovirus to cleave the 32K/170K processing site from the other assessed. The results of the experiments show that the 24K proteinases are virus-specific in cis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-9-2365