@article{mbs:/content/journal/jgv/10.1099/0022-1317-77-9-2365, author = "Shanks, M. and Dessens, J. T. and Lomonossoff, G. P.", title = "The 24 kDa Proteinases of Comoviruses are Virus-specific in cis as Well as in trans", journal= "Journal of General Virology", year = "1996", volume = "77", number = "9", pages = "2365-2369", doi = "https://doi.org/10.1099/0022-1317-77-9-2365", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-9-2365", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "To investigate the specificity of comoviral 24 kDa (‘24K’) proteinases, a full-length cDNA copy of red clover mottle virus (RCMV) RNA 1 has been cloned downstream of a T7 promoter. Translation in rabbit reticulocyte lysates of in vitro transcripts from this clone resulted in the synthesis of a 200K protein which was processed in a manner similar to that of the equivalent protein from cowpea mosaic virus (CPMV). Full-length cDNA clones of the RNA 1 molecules of RCMV and CPMV were used to create hybrid RNA 1 molecules. RNA transcribed in vitro from these hybrids was translated in vitro and the ability of the 24K proteinase from one comovirus to cleave the 32K/170K processing site from the other assessed. The results of the experiments show that the 24K proteinases are virus-specific in cis.", }