1887

Abstract

The M RNA of peanut bud necrosis virus (PBNV; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. It comprised two ORFs in an ambisense organization and terminal inverted repeats. The 3′ large ORF (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kDa which was identified as the glycoprotein precursor (GP) of the G1 and G2 glycoproteins. A comparison of the deduced amino acid sequence of GP revealed 37% identity and 58–59% similarity with that of tomato spotted wilt virus (TSWV, serogroup I) and impatiens necrotic spot virus (INSV, serogroup III), and 21–23% identity and 44–47% similarity with those of other members of the genus . The 5′ small ORF (924 nucleotides in the virus-sense strand) encoded a 34.2 kDa protein which was identified as the non-structural (NSm) protein based on 41–43% identity and 60–63% similarity with that of TSWV and INSV. Defective RNA molecules derived from the genomic M RNA were detected during continuous passage of the virus by sap inoculations.

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1996-09-01
2024-04-18
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References

  1. Adam G., Yeh S. D., Reddy D. V. R., Green S. K. 1993; The serological comparison of tospovirus isolates from Taiwan and India with Impatiens necrotic spot virus and different tomato spotted wilt virus isolates. Archives of Virology 130:237–250
    [Google Scholar]
  2. Bailey J. M., Davidson N. 1976; Methyl mercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  3. de Avila A. C., de Haan P., Kormelink R., Resende R. de. O., Goldbach R. W., Peters D. 1993; Classification of tospoviruses based on phytogeny of nucleoprotein gene sequences. Journal of General Virology 74:153–159
    [Google Scholar]
  4. de Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  6. Elliot R. M. 1990; Molecular biology of the Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  7. Eshita Y., Bishop D. H. L. 1984; The complete sequence of the M RNA of Snowshoe hare bunyavirus reveals the presence of internal hydrophobic domains in the viral glycoprotein. Virology 137:227–240
    [Google Scholar]
  8. Frohman M. A., Dush M. K., Martin G. R. 1988; Rapid production of full-length cDNAs from rare transcripts by amplification using a single gene specific nucleotide primer. Proceedings of the National Academy of Sciences, USA 85:8998–9002
    [Google Scholar]
  9. Grady L. J., Sanders M. L., Campbell W. P. 1987; The sequence of the M RNA of an isolate of La Crosse virus. Journal of General Virology 68:3057–3071
    [Google Scholar]
  10. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  11. Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. 1992; The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. Journal of General Virology 73:2795–2804
    [Google Scholar]
  12. Kormelink R., Storms M., van Lent J., Peters D., Goldbach R. 1994; Expression and subcellular location of the NSm protein of tomato spotted wilt virus (TSWV), a putative movement protein. Virology 200:56–65
    [Google Scholar]
  13. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydrophobic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  14. Law M. D., Speck J., Moyer J. W. 1991; Nucleotide sequence of the 5′ non-coding region and N gene of the S RNA of a serologically distinct tospovirus. Journal of General Virology 72:2597–2601
    [Google Scholar]
  15. Law M. D., Speck J., Moyer J. W. 1992; The M RNA of Impatiens necrotic spot tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188:732–741
    [Google Scholar]
  16. Lees J. F., Pringle C. R., Elliot R. M. 1986; Nucleotide sequence of the Bunyamvera virus M RNA segment: conservation of structural features in the bunyavirus glycoprotein gene product. Virology 148:1–14
    [Google Scholar]
  17. Pang S. Z., Slightom J. L., Gonsalves D. 1993; The biological properties of a distinct tospovirus and sequence analysis of its S RNA. Phytopathology 83:728–733
    [Google Scholar]
  18. Pardigon N., Vialat P., Gerbaud S., Girard M., Bouloy M. 1988; Nucleotide sequence of the M segment of Germiston virus: comparison of the M gene product of several bunyavimses. Virus Research 11:73–85
    [Google Scholar]
  19. Reddy D. V. R., Ratna A. S., Sudarshana M. R., Poul F., Kirankumar I. 1992; Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Annals of Applied Biology 120:279–286
    [Google Scholar]
  20. Resende R. de O., de Haan P., de Avila A. C., Kitajima E. W., Kormelink R., Goldbach R., Peters D. 1991; Generation of envelope and defective interfering RNA mutants of tomato spotted wilt virus by mechanical passage. Journal of General Virology 199:2375–2383
    [Google Scholar]
  21. Ronnholm R., Pettersson R. F. 1987; Complete nucleotide sequence of the M RNA segment of Uukuniemi virus encoding the membrane glycoproteins G1 and G2. Virology 160:191–202
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Satyanarayana T., Mitchell S. E., Reddy D. V. R., Brown S., Kresovich S., Jarret R., Naidu R. A., Demski J. W. 1996; Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses. Archives of Virology 141:85–98
    [Google Scholar]
  24. Yeh S. D., Chang T. F. 1995; Nucleotide sequence of the N gene of watermelon silver mottle vims, a proposed new member of the genus tospovirus. Phytopathology 85:58–64
    [Google Scholar]
  25. Zucker M., Stiegler P. 1981; Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Research 9:133–148
    [Google Scholar]
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