1887

Abstract

The marine brown algal genus contains two species, and . Field populations of both species include plants with infection symptoms caused by DNA viruses. We have established clonal cultures from infected and normal host plants and investigated the properties of the endogenous viruses. Both host species contain virus particles with a hexagonal cross-section and a diameter of ca. 150 nm. The genomes of both virus types consist of double-stranded DNA, approximately 320 kb in size. Restriction digestion with l revealed differences between the two virus genomes. However, PCR experiments suggest that at least one gene, which encodes a major capsid protein, is quite similar in both virus species. In cross-infection experiments the virus did not initiate an infection cycle in . In contrast, the virus infected zoospores. The resulting plants showed aberrant symptoms and produced virus particles which were not infectious. We conclude that the two species are hosts for different, but closely related viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2329
1996-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/9/JV0770092329.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-9-2329&mimeType=html&fmt=ahah

References

  1. Bräutigam M., Klein M., Knippers R., Muller D. G. 1995; Inheritance and meiotic elimination of a virus genome in the host Ectocarpus siliculosus (Phaeophyceae). Journal of Phycology 31:823–827
    [Google Scholar]
  2. Klein M., Lanka S., Muller D. G., Knippers R. 1994; Singlestranded regions in the genome of the Ectocarpus siliculosus virus. Virology 202:1076–1078
    [Google Scholar]
  3. Klein M., Lanka S. T. J., Knippers R., Müller D. G. 1995; Coat protein of the Ectocarpus siliculosus virus. Virology 206:520–526
    [Google Scholar]
  4. Kuhlenkamp R., Müller D. G. 1994; Isolation and regeneration of protoplasts from healthy and virus-infected gametophytes of Ectocarpus siliculosus (Phaeophyceae). Botanica Marina 37:525–530
    [Google Scholar]
  5. Lanka S. T. J., Klein M., Ramsperger U., Müller D. G., Knippers R. 1993; Genome structure of a virus infecting the marine brown alga Ectocarpus siliculosus . Virology 193:802–811
    [Google Scholar]
  6. Müller D. G. 1991; Mendelian segregation of a virus genome during host meiosis in the marine brown alga Ectocarpus siliculosus . Journal of Plant Physiology 137:739–743
    [Google Scholar]
  7. Müller D. G. 1992; Intergeneric transmission of a marine plant DNA virus. Naturwissenschaften 79:37–39
    [Google Scholar]
  8. Müller D. G., Parodi E. 1993; Transfer of a marine DNA virus from Ectocarpus to Feldmannia (Ectocarpales, Phaeophyceae): aberrant symptoms and restitution of the host. Protoplasma 175:121–125
    [Google Scholar]
  9. Müller D. G., Stache B. 1992; Worldwide occurrence of virus infections in filamentous marine brown algae. Helgolander Meeresunter-suchungen 46:1–8
    [Google Scholar]
  10. Müller D. G., Kawai H., Stache B., Lanka S. 1990; A virus infection in the marine brown alga Ectocarpus siliculosus (Phaeophyceae). Botanica Acta 103:72–82
    [Google Scholar]
  11. Müller D. G., Bräutigam M., Knippers R. 1995; Virus infection and persistence of foreign DNA in the marine brown alga Feldmannia simplex (Ectocarpales, Phaeophyceae). Phycologia 35:61–63
    [Google Scholar]
  12. Parodi E. R., Müller D. G. 1994; Field and culture studies on virus infections in Hincksia hincksiae and Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae). European Journal of Phycology 29:113–117
    [Google Scholar]
  13. Sengco M. R., Bräutigam M., Kapp M., Müller D. G. 1996; Detection of virus DNA in Ectocarpus siliculosus and E. fasciculatus from various geographic areas. European Journal of Phycology 31:73–78
    [Google Scholar]
  14. Starr R. C., Zeikus J. A. 1993; UTEX – The culture collection of algae at the University of Texas at Austin. Journal of Phycology 29: Supplement 1–106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2329
Loading
/content/journal/jgv/10.1099/0022-1317-77-9-2329
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error