1887

Abstract

In the present study, a lepidopteran cell line (Ld-652Y, from the gypsy moth, ) exposed to polydnavirus (HfPV) was found to display a variety of cytopathic effects. These included a transient inhibition of cell proliferation, rounding up, aggregation and apoptosis. In addition, unusual paracrystalline structures appeared within the lumen of the rough endoplasmic reticulum; similar structures were observed in the spherulocytes of parasitized . Following Coomassie Blue staining, two new cell-associated polypeptides were detected; one of these, an 8 kDa polypeptide, could also be observed following exposure of Ld-652Y cells to media taken from infected cultures or to cell-free haemolymph from parasitized . After a period of 2–4 weeks, the cell cultures were observed to largely recover from the effects of exposure to virus, and resumed proliferation; ‘transformed’ cell populations tended to form aggregates, and adhered less tightly to the substrate. Viral DNA was stably maintained in all recovered cell lines, possibly in chromosomally integrated form.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2321
1996-09-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2321
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error