1887

Abstract

A single nucleocapsid nuclear poly-hedrosis virus (TnSNPV) isolate was cloned and its replication studied in the BTI-Tn-5B1-4 insect cell line. The BTI-Tn-5B1-4 cells were highly susceptible to TnSNPV infection, with 99% of the cells containing viable polyhedra by 30 h post-inoculation. Viral DNA synthesis was detected by 9 h post-infection (p.i.). Infectious budded virus (BV) was first detected at 13 h p.i. and reached an average maximum titre of 3.875 × 10 p.f.u./ml 27 h p.i. A total of 25 BV structural proteins having apparent molecular masses ranging from 27.5 kDa to 86 kDa were identified. Using [S]methionine pulse-labelling, 19 virus-induced proteins with molecular masses ranging from 27 kDa to 106 kDa were detected from 4 to 28 h p.i. Host cell protein synthesis continued throughout virus replication, although at gradually decreasing rates. Thirty-two structural proteins of occlusion-derived virus ranging in apparent molecular masses from 11 kDa to 98 kDa were identified using silver staining procedures. Digestion of viral DNA with the restriction endonucleases RI, dIII and HI generated 31, 26 and 12 fragments, respectively. Estimates for the molecular mass of the TnSNPV genome ranged from 115.5 to 119.2 kbp. In bioassays performed with neonate larvae, the mean LDs for the TnSNPV and MNPV were 1.5 (±0.3) and 11.0 (±4.0) polyhedra per larva, respectively.

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1996-09-01
2021-10-18
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1989 Current Protocols in Molecular Biology vols 1 & 2 New York: Greene Publishing Associates;
    [Google Scholar]
  2. Bilimoria S. L. 1983; Genomic divergence among single-nucleocapsid nuclear polyhedrosis viruses of plusiine hosts. Virology 127:15–23
    [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
    [Google Scholar]
  4. Bresser J., Gillespie D. 1983; Quantitative binding of covalently closed circular DNA to nitrocellulose in Nal. Analytical Biochemistry 129:357–364
    [Google Scholar]
  5. Cibulsky R. J., Harper J. D., Gudauskas R. T. 1977; Biochemical comparison of virion proteins from five nuclear polyhedrosis viruses infecting plusiine larvae (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 30:303–313
    [Google Scholar]
  6. Davis T. R., Wickham T. J., McKenna K. A., Granados R. R., Shuler M. L., Wood H. A. 1993; Comparative recombinant protein production of eight insect cell lines. In Vitro Cellular & Developmental Biology 29A:388–390
    [Google Scholar]
  7. Erlandson M. A., Carstens E. B. 1983; Mapping early transcription products of Autographa californica nuclear polyhedrosis virus. Virology 126:398–402
    [Google Scholar]
  8. Gettig R. R. 1983; Heliothis spp. baculoviruses: genetic characterization of geographical isolates, in vitro replication and molecular cloning. PhD thesis The Pennsylvania State University; USA:
    [Google Scholar]
  9. Gettig R. R., Burand J. P., Flore P. H., McCarthy W. J., Wood H. A. 1987; Replication of Heliothis zea nuclear polyhedrosis virus in insect cell culture: virus-induced and structural proteins. Journal of General Virology 68:1617–1625
    [Google Scholar]
  10. Granados R. R., Lawler K. A. 1981; In vivo pathway of Autographa californica baculovirus invasion and infection. Virology 108:297–308
    [Google Scholar]
  11. Granados R. R., Lawler K. A., Burand J. P. 1981; Replication of Heliothis zea baculovirus in an insect cell line. Intervirology 16:71–79
    [Google Scholar]
  12. Granados R. R., Derksen A. C. G., Dwyer K. G. 1986; Replication of the Trichoplusia ni granulosis and nuclear polyhedrosis viruses in cell cultures. Virology 152:472–476
    [Google Scholar]
  13. Granados R. R., Guoxun L., Derksen A. C. G., McKenna K. A. 1994; A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus. Journal of Invertebrate Pathology 64:260–266
    [Google Scholar]
  14. Hamblin M., Van Beek N. A. M., Hughes P. R., Wood H. A. 1990; Co-occlusion and persistence of a baculovirus mutant lacking the polyhedrin gene. Applied and Environmental Microbiology 56:3057–3062
    [Google Scholar]
  15. Hill J. E., Faulkner P. 1994; Identification of the gp67 gene of a baculovirus pathogenic to the spruce budworm, Choristoneura fumiferana multinucieocapsid nuclear polyhedrosis virus. Journal of General Virology 75:1811–1813
    [Google Scholar]
  16. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature 226:466–467
    [Google Scholar]
  17. Hughes P. R., Van Beek N. A. M., Wood H. A. 1986; A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctuid larvae. Journal of Invertebrate Pathology 48:187–192
    [Google Scholar]
  18. Knudson D. L., Tinsley T. W. 1978; Replication of a nuclear polyhedrosis virus in a continuous cell line of Spodoptera frugiperda: partial characterization of the viral DNA, comparative DNA–DNA hybridization, and patterns of DNA synthesis. Virology 87:42–57
    [Google Scholar]
  19. McIntosh A. H., Ignoffo C. M. 1981; Replication and infectivity of the single-embedded nuclear polyhedrosis virus, baculovirus Heliothis, in homologous cell lines. Journal of Invertebrate Pathology 37:258–264
    [Google Scholar]
  20. Maniatis T., Fritseh E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Sohi S. S., Percy J., Arif B. M., Cunningham J. C. 1984; Replication and serial passage of a singly enveloped baculovirus of Orgyia leucostigma in homologous cell lines. Intervirology 21:50–60
    [Google Scholar]
  22. Van Beek N. A. M., Hughes P. R. 1986; Determination of the volume ingested by neonate lepidopterous larvae using the fluorescent dye sodium fluorescein. Journal of Invertebrate Pathology 48:249–251
    [Google Scholar]
  23. Volkman L. E. 1983; Occluded and budded Autographa californica nuclear polyhedrosis virus: immunological relatedness of structural proteins. Journal of Virology 46:221–229
    [Google Scholar]
  24. Whitford M., Stewart S., Kuzio J., Faulkner P. 1989; Identification and sequence analysis of a gene encoding gp67, an abundant envelope glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 63:1393–1399
    [Google Scholar]
  25. Wood H. A. 1977; An agar overlay plaque assay method for Autographa californica nuclear polyhedrosis virus. Journal of Invertebrate Pathology 29:304–307
    [Google Scholar]
  26. Wood H. A. 1980a; Isolation and replication of an occlusion-deficient mutant of the Autographa californica nuclear polyhedrosis virus. Virology 105:338–344
    [Google Scholar]
  27. Wood H. A. 1980b; Protease degradation of Auiogmpha caUfornica nuclear polyhedrosis virus proteins. Virology 103:21–27
    [Google Scholar]
  28. Wood H. A., Johnston L. D., Burand J. P. 1982; Inhibition of Autographa californica nuclear polyhedrosis virus replication in high-density Trichoplusia ni cell cultures. Virology 119:245–254
    [Google Scholar]
  29. Yamada K. I., Sherman K. E., Maramorosch K. 1981; In vivo infectivity of early and late passaged Heliothis zea polyhedra produced in tissue culture. Applied Entomology and Zoology 16:504–505
    [Google Scholar]
  30. Young S. Y., Lovell J. S. 1973; Virion proteins of the Trichoplusia ni nuclear polyhedrosis virus. Journal of Invertebrate Pathology 22:471–472
    [Google Scholar]
  31. Zhu G.-K., Zhang H.-J. 1985; The multiplication characteristics of Heliothis armigera baculovirus in the established cell lines. In Proceedings of the 3rd International Cell Culture Congress p 66 Edited by Mitsuhashi J., Kuroda Y., Maramorosch K.
    [Google Scholar]
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