1887

Abstract

A quantitative competitive PCR assay for human herpesvirus 6 (HHV-6) was developed. Firstly, viral burden was determined in the blood of 25 healthy persons. Using 1 µg of DNA, the prevalence of HHV-6 was 36% (9/25). Eight persons had viral loads of ⩽ 32 HHV-6 genomes/µg DNA. The viral burden in the ninth individual was 1.2 × 10 HHV-6 genome copies/µg DNA, which remained constant over a period of 10 months. This demonstrates the persistence of a high HHV-6 load in the absence of apparent disease. Secondly, HHV-6 burden was determined in 100 post-mortem tissues from seven AIDS patients and three controls. For all tissues combined, there was a statistically significant higher median viral load in AIDS patients (56 copies/µg DNA, range 0–43 321) compared to controls (10 copies/µg DNA, range 0–423) ( = 0.04). The precision and reproducibility of this assay will allow hypotheses concerning the pathogenic potential of HHV-6 to be tested quantitatively.

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1996-09-01
2024-04-23
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References

  1. Challoner P. B., Smith K. T., Parker J. D., MacLeod D. L., Coulter S. N., Rose T. M., Schultz E. R., Bennett J. L., Garber R. L., Chang M., Schad P. A., Stewart P. M., Nowinski R. C., Brown J. P., Burmer G. C. 1995; Plaque-associated expression of human herpesvirus 6 in multiple sclerosis. Proceedings of the National Academy of Sciences, USA 92:7440–7444
    [Google Scholar]
  2. Cone R. W., Huang M.-L. W., Ashley R., Corey L. 1993a; Human herpesvirus 6 DNA in peripheral blood cells and saliva from immunocompetent individuals. Journal of Clinical Microbiology 31:1262–1267
    [Google Scholar]
  3. Cone R. W., Hackman R. C., Huang M.-L. W., Bowden R. A., Meyers J. D., Metcalf M., Zeh J., Ashley R., Corey L. 1993b; Human herpesvirus 6 in lung tissue from patients with pneumonitis after bone marrow transplantation. New England Journal of Medicine 329:156–161
    [Google Scholar]
  4. Connor R. I., Mohri H., Cao Y., Ho D. D. 1993; Increased viral burden and cytopathicity correlate temporally with CD4+ T-lym-phocyte decline and clinical progression in human immunodeficiency virus type 1-infected individuals. Journal of Virology 67:1772–1777
    [Google Scholar]
  5. Corbellino M., Lusso P., Gallo R. C., Parravicini C., Galli M., Moroni M. 1993; Disseminated human herpesvirus 6 infection in AIDS. Lancet 342:1242
    [Google Scholar]
  6. Drobyski W. R., Dunne W. M., Burd E. M., Knox K. K., Ash R. C., Horowitz M. M., Flomenberg N., Carrigan D. R. 1993; Human herpesvirus-6 (HHV-6) infection in allogeneic bone marrow transplant recipients: evidence of a marrow-suppressive role for HHV-6 in vivo. Journal of Infectious Diseases 167:735–739
    [Google Scholar]
  7. Drobyski W. R., Knox K. K., Majewski D., Carrigan D. R. 1994; Brief report: fatal encephalitis due to variant B human herpesvirus-6 infection in a bone marrow-transplant recipient. New England Journal of Medicine 330:1356–1360
    [Google Scholar]
  8. Fairfax M. R., Schacker T., Cone R. W., Collier A. C., Corey L. 1994; Human herpesvirus 6 DNA in blood cells of human immunodeficiency virus-infected men: correlation of high levels with high CD4 cells counts. Journal of Infectious Diseases 169:1342–1345
    [Google Scholar]
  9. Fox J. C., Griffiths P. D., Emery V. C. 1992; Quantification of human cytomegalovirus DNA using the polymerase chain reaction. Journal of General Virology 73:2405–2408
    [Google Scholar]
  10. Fox J. C., Kidd I. M., Griffiths P. D., Sweny P., Emery V. C. 1995; Longitudinal analysis of cytomegalovirus load in renal transplant recipients using a quantitative polymerase chain reaction: correlation with disease. Journal of General Virology 76:309–319
    [Google Scholar]
  11. Griffiths P. D. 1992; Studies to define viral cofactors for human immunodeficiency virus. Infectious Agents and Disease 1:237–244
    [Google Scholar]
  12. Griffiths P. D., Emery V. C., Lee C., Johnson M. A., McLaughlin J. 1994; HHV-6 in AIDS. Lancet 343:1104–1105
    [Google Scholar]
  13. Grundy J. E., Shanley J. D., Griffiths P. D. 1987; Is cytomegalovirus interstitial pneumonitis in transplant recipients an immuno-pathological condition?. Lancet ii:996–999
    [Google Scholar]
  14. Hall C. B., Long C. E., Schnabel K. C., Caserta M. T., McIntyre K. M., Costanzo M. A., Knott A., Dewhurst S., Insel R. A., Epstein L. G. 1994; Human herpesvirus-6 infection in children. A prospective study of complications and reactivation. New England Journal of Medicine 331:432–438
    [Google Scholar]
  15. Huang L.-M., Kuo P.-F., Lee C.-Y., Chen J.-Y., Liu M.-Y., Yang C.-S. 1992; Detection of human herpesvirus-6 DNA by polymerase chain reaction in serum or plasma. Journal of Medical Virology 38:7–10
    [Google Scholar]
  16. Knox K. K., Carrigan D. R. 1994; Disseminated active HHV-6 infections in patients with AIDS. Lancet 343:577–578
    [Google Scholar]
  17. Knox K. K., Carrigan D. R. 1995; Active human herpesvirus (HHV-6) infection of the central nervous system in patients with AIDS. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 9:69–73
    [Google Scholar]
  18. Luppi M., Barozzi P., Marasca R., Ceccherini-Nelli L., Torelli G. 1993; Characterization of human herpesvirus 6 genomes from cases of latent infection in human lymphomas and immune disorders. Journal of Infectious Diseases 168:1074–1075
    [Google Scholar]
  19. Lusso P., Gallo R. C. 1995; Human herpesvirus 6 in AIDS. Immunology Today 16:67–71
    [Google Scholar]
  20. Murphy G., Kavanagh T. 1988; Speeding up the sequencing of double-stranded DNA. Nucleic Acids Research 16:5198
    [Google Scholar]
  21. Secchiero P., Carrigan D. R., Asano Y., Benedetti L., Crowley R. W., Komaroff A. L., Gallo R. C., Lusso P. 1995a; Detection of human herpesvirus 6 in plasma of children with primary infection and immunosuppressed patients by polymerase chain reaction. Journal of Infectious Diseases 171:273–280
    [Google Scholar]
  22. Secchiero P., Zella D., Crowley R. W., Gallo R. C., Lusso P. 1995b; Quantitative PCR for human herpesviruses 6 and 7. Journal of Clinical Microbiology 33:2124–2130
    [Google Scholar]
  23. Torelli G., Barozzi P., Marasca R., Cocconcelli P., Merelli E., Ceccherini-Nelli L., Ferrari S., Luppi M. 1995; Targeted integration of human herpesvirus 6 in the p arm of chromosome 17 of human peripheral blood mononuclear cells in vivo. Journal of Medical Virology 46:178–188
    [Google Scholar]
  24. Wakefield A. J., Fox J. D., Sawyerr A. M., Taylor J. E., Sweenie C. H., Smith M., Emery V. C., Hudson M., Tedder R. S., Pounder R. E. 1992; Detection of herpesvirus DNA in the large intestine of patients with ulcerative colitis and Crohn’s disease using the nested polymerase chain reaction. Journal of Medical Virology 38:183–190
    [Google Scholar]
  25. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. 1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet i:1065–1067
    [Google Scholar]
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