RT Journal Article SR Electronic(1) A1 Dangoria, Nandita S. A1 Breau, Walter C. A1 Anderson, Howard A. A1 Cishek, Dawn M. A1 Norkin, Leonard C.YR 1996 T1 Extracellular Simian Virus 40 Induces an ERK/MAP Kinase-independent Signalling Pathway that Activates Primary Response Genes and Promotes Virus Entry JF Journal of General Virology, VO 77 IS 9 SP 2173 OP 2182 DO https://doi.org/10.1099/0022-1317-77-9-2173 PB Microbiology Society, SN 1465-2099, AB Simian virus 40 (SV40) binding to growth-arrested cells activated an intracellular signalling pathway that induced the up-regulation of the primary response genes c-myc, c-jun and c-sis within 30 min and of JE within 90 min. The up-regulation of the primary response genes occurred in the presence of cycloheximide and when UV-inactivated SV40 was adsorbed to cells. SV40 binding did not activate Raf or mitogen-activated protein kinase (MAP/ERK1), or mobilize intracellular Ca2+. The SV40-induced up-regulation of c-myc and c-jun was blocked by the tyrosine kinase inhibitor, genistein, and by the protein kinase C (PKC) inhibitor, calphostin C, but not by expression of the MAP kinase-specific phosphatase, MKP-1. These results suggest that the SV40-induced signalling pathway includes the activities of a tyrosine kinase and a Ca2+-independent isoform of PKC, but not of Raf or MAP kinase. Finally, SV40 infectious entry into cells was specifically and reversibly blocked by genistein., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-9-2173