1887

Abstract

Using the whole body section hybridization technique, we monitored the organ- and age-specific pattern of replication of hamster polyomavirus (HaPV) DNA in a colony of Syrian hamsters, which are susceptible to lymphoma induction. Three phases of viral infection and replication could be distinguished: first, a phase of acute infection characterized by high levels of replication of HaPV DNA in the haemopoietic organs and the liver. This culminated 5 to 7 days post-infection (p.i.); second, at 10 days p.i., a phase of viral clearance became evident; and finally, a third phase reflected both the restriction of HaPV replication in adult hamsters and the accumulation of HaPV DNA at sites of tumour development. A remarkable conformity was observed between the tissue specificity of viral replication and the induced tumour profile: high levels of replication of HaPV DNA were restricted to cells of the haemopoietic system and lymphoid tumours were induced. As shown by hybridization, the viral infection in non-haemopoietic organs was due to the dissemination of HaPV-infected blood cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2165
1996-09-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/9/JV0770092165.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-9-2165&mimeType=html&fmt=ahah

References

  1. Atencio I. A., Shadan F. F., Zhou X. J., Vaziri N. D., Villarreal L. P. 1993; Adult mouse kidneys become permissive to acute polyomavirus infection and reactivate persistent infections in response to cellular damage and regeneration. Journal of Virology 67:1424–1432
    [Google Scholar]
  2. Böttger M., Bierwolf D., Wunderlich V., Graffi A. 1971; New calibration correlations for molecular weights of circular DNA: the molecular weight of the DNA of an oncogenic papovavirus of the Syrian hamster. Biochimica el Biophysica Acta 232:21–31
    [Google Scholar]
  3. Dawe C. J., Freund R., Mandel G., Balmer-Hofer K., Talmage D. A., Benjamin T. L. 1987; Variations in polyoma virus genotype in relation to tumor induction in mice: characterization of wild type strains with widely differing tumor profiles. American Journal of Pathology 127:243–261
    [Google Scholar]
  4. de la Roche Saint Andre Ch., Harper F., Feunteun J. 1990; Analysis of the hamster polyomavirus infection in vitro: host-restricted productive cycle. Virology 177:532–540
    [Google Scholar]
  5. Delmas V., Bastien C., Scherneck S., Feunteun J. 1985; A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties. EMBO Journal 4:1279–1286
    [Google Scholar]
  6. Dubensky T. W., Villarreal L. P. 1984a; Detection of DNA and RNA virus genomes in organ systems of whole mice: patterns of mouse organ infection by polyomavirus. Journal of Virology 50:779–783
    [Google Scholar]
  7. Dubensky T. W., Villarreal L. P. 1984b; The primary site of replication alters the eventual site of persistent infection by polyomavirus in mice. Journal of Virology 50:541–546
    [Google Scholar]
  8. Fey F., Schramm T., Bender E., Rudolph M. 1970; Unter-suchungen zur Histogenese Hamster-Papovavirus-induzierter Leukosen. Archiv für Geschwulstforschung 36:10–18
    [Google Scholar]
  9. Goutebroze L., Feunteun J. 1992; Transformation by hamster polyomavirus: identification and functional analysis of the early genes. Journal of Virology 66:2495–2504
    [Google Scholar]
  10. Graffi A., Schramm T., Bender E., Bierwolf D., Graffi I. 1967; Über einen neuen virushaltigen Hauttumor beim Goldhamster. Archiv für Geschwulstforschung 30:277–283
    [Google Scholar]
  11. Graffi A., Schramm T., Graffi I., Bierwolf D., Bender E. 1968; Virus-associated skin tumors of the Syrian hamster: preliminary note. Journal of the National Cancer Institute 40:867–873
    [Google Scholar]
  12. Graffi A., Bender E., Schramm T., Graffi I., Bierwolf D. 1970; Studies on the hamster papilloma and the hamster virus lymphoma. Comparative Leukemia Research Bibliotheca Haematologica, Basel 36:293–303
    [Google Scholar]
  13. Lipkin W. I., Villarreal L. P., Oldstone M. B. A. 1989; Whole animal section in situ hybridization and protein blotting: new tools in molecular analysis of animal models for human disease. Current Topics in Microbiology and Immunology 143:33–54
    [Google Scholar]
  14. Mazur S., Goodhardt M., Feunteun J., de la Roche Saint Andre Ch. 1994; In vivo replication of the hamster polyomavirus genome and generation of specific deletions in the process of lymphomagenesis. Journal of Virology 68:5629–5637
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  16. Scherneck S., Delmas V., Vogel F., Feunteun J. 1987; Induction of lymphomas by the hamster papovavirus correlates with massive replication of nonrandomly deleted extrachromosomal viral genomes. Journal of Virology 61:3992–3998
    [Google Scholar]
  17. Southern P. J., Blount P., Oldstone M. B. A. 1984; Analysis of persistent virus infections by in situ hybridization to whole-mouse sections. Nature 312:555–558
    [Google Scholar]
  18. Villarreal L. P. 1991; Relationship of eukaryotic DNA replication to committed gene expression: general theory for gene control. Microbiological Reviews 55:512–542
    [Google Scholar]
  19. Vogel F., Rhode K., Scherneck S., Bastien C., Delmas V., Feunteun J. 1986; The hamster papovavirus: evolutionary relationships with other polyomaviruses. Virology 154:335–343
    [Google Scholar]
  20. Wirth J. J., Amalfitano A., Gross R., Oldstone M. B. A., Fluck M. M. 1992; Organ- and age-specific replication of polyomavirus in mice. Journal of Virology 66:3278–3286
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2165
Loading
/content/journal/jgv/10.1099/0022-1317-77-9-2165
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error