1887

Abstract

The gene encoding the muscovy duck parvovirus (DPV)-strain 89384 capsid proteins VP2 and VP3 was cloned in a baculovirus expression system and expressed in insect cells. The recombinant proteins were found to react with specific anti-DPV serum by Western blotting and to be located in the nucleus of insect cells (Sf9) as shown by immunofluorescence. Empty virus-like particles (VLPs) identical in size and appearance to DPV virions were observed by electron microscopy. The antigenicity and immunogenicity of the recombinant proteins were evaluated by ELISA and seroneutralization. Immunization of 3-week-old muscovy ducklings induced anti-DPV antibodies; neutralizing antibody titres were consistent with those observed in ducklings inoculated with a commercial inactivated vaccine. The way to develop these promising results is discussed.

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1996-09-01
2021-10-18
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References

  1. Becerra S. P., Koczot F., Fabisch P., Rose J. A. 1988; Synthesis of adeno-associated virus structural proteins requires both alternative mRNA splicing and alternative initiations from a single transcript. Journal of Virology 62:2745–2754
    [Google Scholar]
  2. Cassinotti P., Weitz M., Tratschin J.-D. 1988; Organization of the adeno-associated virus (AAV) capsid gene: mapping of minor spliced mRNA coding for virus capsid protein 1. Virology 167:176–184
    [Google Scholar]
  3. Christensen J., Storgaard T., Bloch B., Alexandersen S., Aasted B. 1993; Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system. Journal of Virology 67:229–238
    [Google Scholar]
  4. Christensen J., Alexandersen S., Bloch B., Aasted B., Uttenthal Å. 1994; Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink. Journal of General Virology 75:149–155
    [Google Scholar]
  5. Dalibart V., Plassiart G., Cherel Y., Hurtrel M., Wyers M. 1993; Caractérisation des lesions histologiques de la Parvovirose spontanee du canard de Barbarie (Cairina moschata). Diagnostic histopathologique différentiel avec la Reovirose. Recueil de Medecine Veterinaire 169:763–772
    [Google Scholar]
  6. Drouin P., Toux J. Y., L’Hospitalier R., Guittet M., Bennejean G. 1991; D′après les donnèes du réseau national d′observations épidémiologiques en aviculture, Evolution de l′incidence de la maladie de Derzsy en 1989 et 1990. L’Aviculteur 521:36–40
    [Google Scholar]
  7. Fournier D., Gaudry D. 1992; Muscovy duck parvovirus (MDP) in France: field vaccination trials. Proceedings of the 9th International symposium on Waterfowl Pisa, Italy, 16–18 September 36–41
    [Google Scholar]
  8. Jestin V. 1990; Le point sur lépizootie actuelle de maladie de Derzsy chez le canard de Barbarie. L'Aviculteur 516:77–78
    [Google Scholar]
  9. Jestin V., Le Bras M. O., Cherbonnel M., Le Gall G., Bennejean G. 1991a; Isolement de virus de la maladie de Derzsy tres pathogenes chez le canard de Barbarie. Recueil de Medecine Veterinaire 167:849–857
    [Google Scholar]
  10. Jestin V., Cherbonnel M., Le Bras M. O., Morin Y., Petit J., Le Coq L., Le Moal L., Jarnet G., Ogier R., Quitin E. 1991b; Contrôle de l′immunité contre la maladie de Derzsy. L’Aviculteur 521:59–60
    [Google Scholar]
  11. Jestin V., Le Bras M. O., Cherbonnel M. 1992; Control of muscovy duck parvovirosis by vaccination. European Commission Proceedings: New and Evolving Virus Diseases of Poultry Brussels, Belgium, 15-16 December 167–181
    [Google Scholar]
  12. Jestin V., Le Bras M. O., Cherbonnel M. 1993; Controle de laparvovirose du canard par la vaccination. Proceeding litres journees de la recherche sur les palmipèdes à foie gras INTRA/ITAVI, Bordeaux, France, 27–28 avril 119–137
    [Google Scholar]
  13. Jestin V., Le Gall-Recule G., Arnauld C., Cherbonnel M., Jestin A. 1995; Detection of muscovy duck parvovirus DNA by the polymerase chain reaction. Immunobiology of viral infections Proceedings of the 3rd Congress of the European Society for Veterinary Virology Interlaken 1994 528–532 Edited by Schwyzer M.
    [Google Scholar]
  14. Kajigaya S., Fujii H., Field A. M., Rosenfeld S., Anderson L. J., Shimada T., Young N. S. 1991; Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunogenically similar to native virions. Proceedings of the National Academy of Sciences, USA 88:4646–4650
    [Google Scholar]
  15. Le Gall-Reculé G., Jestin V. 1994; Biochemical and genomic characterization of muscovy duck parvovirus. Archives of Virology 139:121–131
    [Google Scholar]
  16. Le Gall-Reculé G., Jestin V. 1995; Production of digoxigenin-labelled DNA probe for detection of muscovy duck parvovirus. Molecular and Cellular Probes 9:39–44
    [Google Scholar]
  17. Lopez de Turiso J. A., Cortes E., Martinez C., Ruiz de Ybanez R., Simarro I., Vela C., Casal I. 1992; Recombinant vaccine for canine parvovirus in dogs. Journal of Virology 66:2748–2753
    [Google Scholar]
  18. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Biotechnology 6:47–55
    [Google Scholar]
  19. Martinez C., Dalsgaard K., Lopez de Turiso J. A., Cortes E., Vela C., Casal J. I. 1992; Production of porcine parvovirus empty capsids with high immunogenic activity. Vaccine 10:684–690
    [Google Scholar]
  20. Ruffing M., Zentgraf H., Kleinschmidt J. A. 1992; Assembly of viruslike particles by recombinant structural proteins of adeno-associated virus type 2 in insect cells. Journal of Virology 66:6922–6930
    [Google Scholar]
  21. Saliki J. T., Mizak B., Flore H. P., Gettig R. R., Burand J. P., Carmichael L. E., Wood H. A., Parrish C. R. 1992; Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs. Journal of General Virology 73:369–374
    [Google Scholar]
  22. Trempe J. P., Carter B. J. 1988; Alternate mRNA splicing is required for synthesis of adeno-associated virus VPl capsid protein. Journal of Virology 62:3356–3363
    [Google Scholar]
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