1887

Abstract

We are investigating the binding of a series of monoclonal antibodies to native and detergent-treated human T-lymphotropic virus I (HTLV-I) envelope proteins to explore their conformation. A comparison of our data with previously published findings suggests that a central neutralization domain (aa 175–200) is folded such that only short stretches are exposed at the surface of the native envelope protein complex. However, the complete domain becomes accessible after treatment with mild non-ionic detergents, suggesting that envelope subunit interaction may partially obscure this domain. We further provide immunochemical evidence that a region containing a heptad repeat in the extracellular part of the transmembrane protein is folded towards the interior of the HTLV-I envelope complex.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2025
1996-09-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/9/JV0770092025.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-9-2025&mimeType=html&fmt=ahah

References

  1. Baba E., Nakamura M., Tanaka Y., Kuroki M., Itoyama Y., Nakano S., Niho Y. 1993; Multiple neutralising B-cell epitopes of human T-cell leukemia virus type 1 (HTLV-I). Joumal of Immunology 151:1013–1024
    [Google Scholar]
  2. Carr C. M., Kim P. S. 1993; A spring loaded mechanism for the conformational change of influenza hemagglutinin. Cell 73:823–832
    [Google Scholar]
  3. Carrington C. V. F., Weiss R. A., Schulz T. F. 1994; A truncated HTLV-I envelope protein, lacking the hydrophobic membrane anchor domain, is associated with cellular membranes and virions. Virology 202:61–69
    [Google Scholar]
  4. Chen S. S., Lee C. N., Lee W. R., McIntosh K., Lee T. H. 1993; Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein. Journal of Virology 67:3615–3619
    [Google Scholar]
  5. Delwart E. L., Mosialos G., Gilmore T. 1990; Retroviral envelope glycoproteins contain a ‘leucine zipper’-like repeat. AIDS Research and Human Retroviruses 6:703–706
    [Google Scholar]
  6. Dubay J. W., Roberts S. J., Brody B., Hunter E. 1992; Mutations in the leucine zipper of the human immunodeficiency virus type I transmembrane glycoprotein affect fusion and infectivity. Journal of Virology 66:4748–4756
    [Google Scholar]
  7. Hattori S., Kiyokawa T., Imagawa K., Shimizu F., Hashimura E., Seiki M., Yoshida M. 1984; Identification of gag and env gene products of human T-cell leukemia virus (HTLV). Virology 136:338–347
    [Google Scholar]
  8. Kuroki M., Nakamura M., Itoyama Y., Tanaka Y., Shiraki H., Baba E., Esaki T., Tatsumoto T., Nagafuchi S., Nakano S., Niho Y. 1992; Identification of new epitopes recognized by human monoclonal antibodies with neutralizing and antibody-dependent cellular cytotoxicity activities specific for human T cell leukemia virus type 1. Journal of Immunology 149:940–948
    [Google Scholar]
  9. Lee T. H., Coligan J. E., Homma T., McLane M. F., Tachibana N., Essex M. 1984; Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection. Proceedings of the National Academy of Sciences, USA 81:3856–3860
    [Google Scholar]
  10. Matsushita S., Robert-Guroff M., Trepel J., Cossman J., Mitsuya H., Broder S. 1986; Human monoclonal antibody directed against an envelope glycoprotein of human T-cell leukemia virus type I. Proceedings of the National Academy of Sciences, USA 83:2672–2676
    [Google Scholar]
  11. Moore J. P. 1990; Simple methods for monitoring HIV-1 and HIV-2 gp!20 binding to soluble CD4 by enzyme-linked immunosorbent assay: HIV-2 has a 25 fold lower affinity than HIV-1 for soluble CD4. AIDS 4:297–305
    [Google Scholar]
  12. Moore J. P., Wallace L. A., Follett E. A., McKeating J. A. 1989; Anenzyme-linked immunosorbent assay for antibodies to the envelope glycoproteins of divergent strains of HIV-1. AIDS 3:155–163
    [Google Scholar]
  13. Paine E., Gu R., Ratner L. 1994; Structure and expression of the human T-cell leukemia virus type 1 envelope protein. Virology 199:331–338
    [Google Scholar]
  14. Palker T. J., Tanner M. E., Scearce R. M., Streilein R. D., Clark M. E., Haynes B. F. 1989; Mapping of immunogenic regions of human T-cell leukemia virus type I (HTLV-I) gp46 and gp21 envelope glycoproteins with encoded synthetic peptides and a monoclonal antibody to gp46. Journal of Immunology 142:971–978
    [Google Scholar]
  15. Paul N. L., Marsh M., McKeating J. A., Schulz T. F., Liljestrom P., Garoff H., Weiss R. A. 1993; Expression of HIV-1 envelope glycoprotein by Semliki Forest virus vectors. AIDS Research and Human Retroviruses 9:963–970
    [Google Scholar]
  16. Pique C., Pham D., Tursz T., Dokhelar M. C. 1992; Human T-cell leukemia virus type I envelope protein maturation process: requirements for syncytium formation. Journal of Virology 66:906–913
    [Google Scholar]
  17. Ralston S., Hoeprich P., Akita R. 1989; Identification and synthesis of the epitope for a human monoclonal antibody which can neutralize human T-cell leukemia/lymphotropic virus type I. Journal of Biological Chemistry 264:16343–16346
    [Google Scholar]
  18. Sattentau Q. J., Zolla-Pazner S., Poignard P. 1995; Epitope exposure on functional, oligomeric HIV-1 gp41 molecules. Virology 206:713–717
    [Google Scholar]
  19. Tanaka Y., Tanaka R., Terada E., Koyanagi Y., Miyano-Kurosaki N., Yamamoto N., Baba E., Nakamura M., Shida H. 1994; Induction of antibody responses that neutralize human T-cell leukemia virus type I infection in vitro and in vivo by peptide immunization. Journal of Virology 68:6323–6331
    [Google Scholar]
  20. Wild C., Oas T., McDanal C., Bolognesi D., Matthews T. 1992; A synthetic peptide inhibitor of human immunodeficiency virus replication: correlation between solution structure and viral inhibition. Proceedings of the National Academy of Sciences, USA 89:10537–10541
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2025
Loading
/content/journal/jgv/10.1099/0022-1317-77-9-2025
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error