1887

Abstract

Multivesicular bodies (MVB) are membranous cytoplasmic inclusions that are invariably associated with tombusvirus infections regardless of the virus species, the host, or the tissue examined. MVB are virus-induced structures since they are absent from tissues of healthy plants and are always present both in infected plants and protoplasts. MVB derive from peroxisomes in cells infected by a number of tombusviruses including cymbidium ringspot virus (CymRSV) and from mitochondria in cells infected by another tombusvirus, carnation Italian ringspot virus (CIRV). By using common restriction sites in full-length infectious clones, hybrid clones of these two viruses were constructed. In addition, a mutant of CIRV was prepared in which the protein encoded by the first open reading frame was shortened by 22 amino acids. All mutant transcripts were viable and infected plants. Infected leaf tissue samples were collected, processed for thin sectioning, and observed in the electron microscope. The origin of MVB was shown to be under the control of the 5′ region of the viral genome. A sequence as short as about 600 nucleotides in ORF 1 contained the determinants for formation of MVB from peroxisomes or mitochondria.

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1996-08-01
2024-03-29
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References

  1. Appiano A., D’Agostino G., Redolfi P., Pennazio S. 1981; Sequence of cytological events during the process of local lesion formation in the tomato bushy stunt virus–Gomphrena globosa hypersensitive system. Journal of Ultrastrudure Research 76:173–180
    [Google Scholar]
  2. Appiano A., D’Agostino G., Bassi M., Barbieri N., Viale G., Dell’Orto P. 1986; Origin and function of tomato bushy stunt virus-induced inclusion bodies. II. Quantitative electron microscope autoradiography and immunogold cytochemistry. Journal of Ultrastrudure Research 97:31–38
    [Google Scholar]
  3. Burgyan J., Nagy P., Russo M. 1990; Synthesis of infectious RNA from full-length cDNA to RNA of cymbidium ringspot tombusvirus. Journal of General Virology 71:1857–1860
    [Google Scholar]
  4. Dalmay T., Rubino L., Burgyan J., Kollar A., Russo M. 1993a; Functional analysis of cymbidium ringspot virus genome. Virology 194:697–704
    [Google Scholar]
  5. Dalmay T., Russo M., Burgyan J. 1993b; Repair in vivo of altered 3′ terminus of cymbidium ringspot tombusvirus RNA. Virology 192:551–555
    [Google Scholar]
  6. De Graaff M., Jaspars E. M. J. 1994; Plant viral RNA synthesis in cell-free systems. Annual Review of Phytopathology 32:311–335
    [Google Scholar]
  7. Di Franco A., Russo M., Martelli G. P. 1984; Ultrastructure and origin of multivesicular bodies induced by carnation Italian ringspot virus. Journal of General Virology 65:1233–1237
    [Google Scholar]
  8. Grieco F., Burgyan J., Russo M. 1989; The nucleotide sequence of cymbidium ringspot virus RNA. Nucleic Acids Research 17:6383
    [Google Scholar]
  9. Heaton L. A., Carrington J. C., Morris T. J. 1989; Turnip crinkle virus infection from RNA synthesized in vitro . Virology 170:214–218
    [Google Scholar]
  10. Hopp T. P., Woods K. R. 1983; A computer program for predicting protein antigenic determinants. Molecular Immunology 20:483–490
    [Google Scholar]
  11. Kollar A., Burgyan J. 1994; Evidence that ORF 1 and 2 are the only virus-encoded replicase genes of cymbidium ringspot tombusvirus. Virology 201:169–172
    [Google Scholar]
  12. Koonin E. V. 1991; The phytogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. Journal of General Virology 72:2197–2206
    [Google Scholar]
  13. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. Lesemann D.-E. 1988; Cytopathology. In The Plant Viruses. The Filamentous Plant Viruses pp 179–235 Edited by Milne R. G. New York: Plenum Press;
    [Google Scholar]
  16. Lupo R., Rubino L., Russo M. 1994; Immunodetection of the 33K/92K polymerase proteins in cymbidium ringspot virus-infected and in transgenic plant tissue extracts. Archives of Virology 138:135–142
    [Google Scholar]
  17. Martelli G. P., Russo M. 1977; Plant virus inclusion bodies. Advances in Virus Research 21:175–266
    [Google Scholar]
  18. Martelli G. P., Russo M. 1984; Use of thin sectioning for visualization and identification of plant viruses. Methods in Virology 8:143–224
    [Google Scholar]
  19. Martelli G. P., Russo M. 1985; Virus-host relationships: sympto- matological and ultrastructural aspects. In The Plant Viruses. Polyhedral Virions with Tripartite Genomes pp 163–205 Edited by Francki R. I. B. New York: Plenum Press;
    [Google Scholar]
  20. Martelli G. P., Di Franco A., Russo M. 1984; The origin of multivesicular bodies in tomato bushy stunt virus-infected Gomphrena globosa plants. Journal of Ultrastructure Research 88:275–281
    [Google Scholar]
  21. Martelli G. P., Gallitelli D., Russo M. 1988; Tombusviruses. In The Plant Viruses. Polyhedral Virions with Monopartite RNA Genomes pp 13–72 Edited by Koenig R. New York: Plenum Press;
    [Google Scholar]
  22. Rubino L., Burgyan J., Russo M. 1995; Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs. Archives of Virology 140:2027–2039
    [Google Scholar]
  23. Russo M., Di Franco A., Martelli G. P. 1983; The fine structure of cymbidium ringspot virus infections in host tissues. III. Role of peroxisomes in the genesis of multivesicular bodies. Journal of Ultra-structure Research 82:52–63
    [Google Scholar]
  24. Russo M., Di Franco A., Martelli G. P. 1987; Cytopathology in the identification and classification of tombusviruses. Intervirology 28:134–143
    [Google Scholar]
  25. Russo M., Burgyan J., Martelli G. P. 1994; Molecular biology of Tombusviridae. Advances in Virus Research 44:381–428
    [Google Scholar]
  26. Scholthof H. B., Morris T. J., Jackson A. O. 1993; The capsid protein gene of tomato bushy stunt virus is dispensable for systemic movement and can be replaced for localized expression of foreign genes. Molecular Plant–Microbe Interactions 6:309–322
    [Google Scholar]
  27. Scholthof H. B., Scholthof K.-B. G., Jackson A. O. 1995a; The tomato bushy stunt virus replicase proteins are coordinately expressed and membrane associated. Virology 208:365–369
    [Google Scholar]
  28. Scholthof H. B., Scholthof K.-B. G., Kikkert M., Jackson A. O. 1995b; Tomato bushy stunt virus spread is regulated by two nested genes that function in cell-to-cell movement and host-dependent systemic invasion. Virology 213:425–438
    [Google Scholar]
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