Tomato golden mosaic virus open reading frame is genetically distinct from its analogue in monopartite geminiviruses Free

Abstract

Tomato golden mosaic virus (TGMV) is a bipartite geminivirus with six well-characterized genes. An additional open reading frame (ORF), lies within the essential gene. Recent studies of monopartite, dicot-infecting geminiviruses have revealed that mutations in their analogous ORFs have host-specific effects on infectivity, symptomatology, virus movement and/or viral DNA accumulation. We have investigated whether TGMV has a similar host-specific requirement for . The phenotypes of three TGMV mutants were determined in a range of hosts, which included species that revealed mutant phenotypes for monopartite geminiviruses. Each TGMV mutant was indistinguishable from wild-type TGMV in all hosts tested. Additional analyses of double mutants revealed no evidence for functional redundancy between and the or genes. In contrast to the putative C4 proteins of monopartite geminiviruses, TGMV AL4, if it is expressed, is either non-functional, or functionally redundant with an essential TGMV gene product.

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1996-08-01
2024-03-29
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References

  1. Brough C. L., Hayes R. J., Morgan A. J., Coutts R. H. A., Buck K. W. 1988; Effects of mutagenesis in vitro on the ability of cloned tomato golden mosaic virus DNA to infect Nicotiana benthamiana plants. Journal of General Virology 69:503–514
    [Google Scholar]
  2. Elmer J. S., Brand L., Sunter G., Gardiner W. E., Bisaro D. M., Rogers S. G. 1988; Genetic analysis of the tomato golden mosaic virus. II. The product of the AL1 coding sequence is required for replication. Nucleic Acids Research 16:7043–7060
    [Google Scholar]
  3. Etessami P., Saunders K., Watts J., Stanley J. 1991; Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A. Journal of General Virology 72:1005–1012
    [Google Scholar]
  4. Fontes E. P. B., Gladfelter H. J., Schaffer R. L., Petty I. T. D., Hanley-Bowdoin L. 1994; Geminivirus replication origins have a modular organization. Plant Cell 6:405–416
    [Google Scholar]
  5. Gardiner W. E., Sunter G., Brand L., Elmer J. S., Rogers S. G., Bisaro D. M. 1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development. EMBO Journal 7:899–904
    [Google Scholar]
  6. Gröning B. R., Hayes R. J., Buck K. W. 1994; Simultaneous regulation of tomato golden mosaic virus coat protein and AL1 gene expression: expression of the AL4 gene may contribute to suppression of the AL1 gene. Journal of General Virology 75:721–726
    [Google Scholar]
  7. Hamilton W. D. O., Stein V. E., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  8. Hong Y., Stanley J. 1995; Regulation of African cassava mosaic virus complementary-sense gene expression by N-terminal sequences of the replication-associated protein AC1. Journal of General Virology 76:2415–2422
    [Google Scholar]
  9. Jupin I., de Kouchkovsky F., Jouanneau F., Gronenborn B. 1994; Movement of tomato yellow leaf curl geminivirus (TYLCV): involvement of the protein encoded by ORF C4. Virology 204:82–90
    [Google Scholar]
  10. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. (editors) 1995 Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
    [Google Scholar]
  11. Padidam M., Beachy R. N., Fauquet C. M. 1995; Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76:25–35
    [Google Scholar]
  12. Rigden J. E., Krake L. R., Rezaian M. A., Dry I. B. 1994; ORF C4 of tomato leaf curl geminivirus is a determinant of symptom severity. Virology 204:847–850
    [Google Scholar]
  13. Schaffer R. L., Miller C. G., Petty I. T. D. 1995; Virus and host- specific adaptations in the BL1 and BR1 genes of bipartite geminiviruses. Virology 214:330–338
    [Google Scholar]
  14. Stanley J., Latham J. R. 1992; A symptom variant of beet curly top geminivirus produced by mutation of open reading frame C4. Virology 190:506–509
    [Google Scholar]
  15. Stanley J., Latham J. R., Pinner M. S., Bedford I., Markham P. G. 1992; Mutational analysis of the monopartite geminivirus beet curly top virus. Virology 191:396–405
    [Google Scholar]
  16. Sung Y. K., Coutts R. H. A. 1995; Mutational analysis of potato yellow mosaic geminivirus. Journal of General Virology 76:1773–1780
    [Google Scholar]
  17. Sunter G., Hartitz M. D., Hormuzdi S. G., Brough C. L., Bisaro D. M. 1990; Genetic analysis of tomato golden mosaic virus: ORF AL2 is required for coat protein accumulation while ORF AL3 is necessary for efficient DNA replication. Virology 179:69–77
    [Google Scholar]
  18. Sunter G., Bisaro D. M. 1989; Transcription map of the B genome component of tomato golden mosaic virus and comparison with A component transcripts. Virology 173:647–655
    [Google Scholar]
  19. Sunter G., Bisaro D. M. 1992; Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell 4:1321–1331
    [Google Scholar]
  20. Torres-Pacheco I., Garzón-Tiznado J. A., Herrera-Estrella L., Rivera-Bustamante R. F. 1993; Complete nucleotide sequence of pepper huasteco virus: analysis and comparison with bipartite geminiviruses. Journal of General Virology 74:2225–2231
    [Google Scholar]
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