1887

Abstract

Human herpesvirus 7 (HHV-7) is a recently isolated herpesvirus that has been shown to be related to human cytomegalovirus and human herpesvirus 6 and to be a member of the betaherpesvirus subgroup. Here we report the cloning, restriction endonuclease mapping and partial sequence analysis of HHV-7 strain JI DNA. Virus particles were obtained from the supernatant of infected SupT1 cells, the DNA isolated by proteinase K treatment-phenol extraction, and full-length viral DNA was purified and isolated on a pulsed-field gel. Aliquots of this highly purified material were treated in the following ways: () sonicated and end-repaired to create short randomly sheared fragments for cloning into M13mp18-l vector DNA; () cut with RI for cloning into RI-cut λZAPII or λDASHII vectors; () cut with HI for cloning into HI-cut λZAP-Express or λDASHII vectors. Partial nucleotide sequencing of the M13 clones followed by detection of open reading frames and their translation allowed the identification of homologues through FASTA searches of the database. Relevant M13 clones were used as probes to isolate corresponding λ phage clones, which could tentatively be mapped to the genome on the basis of presumed genetic collinearity between HHV-7 and HHV-6. Genomic ‘walking’ between RI and HI λ genomic libraries enabled overlapping neighbouring clones to be identified and mapped. Each of these clones was analysed to map HI, RI, l, l and l restriction endonuclease sites to provide complete endonuclease maps for the entire genome.

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1996-08-01
2022-01-28
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References

  1. Asano Y., Suga S., Yoshikawa T., Yazaki T., Uchikawa T. 1995; Clinical features and viral excretion in an infant with primary human herpesvirus 7 infection. Pediatrics 95:187–190
    [Google Scholar]
  2. Bankier A. T., Weston K. M., Barrell B. G. 1987; Random cloning and sequencing by the M13/dideoxynucleotide chain termination method. Methods in Enzymology 155:51–93
    [Google Scholar]
  3. Bememan Z. N., Ablashi D. V., Li G., Eger-Fletcher M., Reitz M. S., Hung C.-L., Brus I., Kamaroff A.-L., Gallo R. C. 1992a; Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. Proceedings of the National Academy of Sciences, USA 89:10552–10556
    [Google Scholar]
  4. Bememan Z. N., Gallo R. C., Ablashi D. V., Frenkel N., Katsafanas G., Kramarsky B., Brus I. 1992b; Human herpesvirus 7 (HHV-7) strain Ji: independent confirmation of HHV-7. Journal of Infectious Diseases 166:690–691
    [Google Scholar]
  5. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchinson C. A., Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the protein coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  6. Clark D. A., Freeland J. M. L., Mackie P. L. K., Jarrett R. F., Onions D. E. 1993; Prevalence of antibody to human herpesvirus 7 by age. Journal of Infectious Diseases 168:251–252
    [Google Scholar]
  7. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  8. Efstathiou S., Lawrence G. L., Brown C. M., Barrell B. G. 1992; Identification of homologues to the human cytomegalovirus US22 gene family in human herpesvirus 6. Journal of General Virology 73:1661–1671
    [Google Scholar]
  9. Feinberg A. D., Vogelstein B. 1983; A technique for radiolabelling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  10. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. 1990; Isolation of a new herpesvirus from human CD4 + T cells. Proceedings of the National Academy of Sciences, USA 87:748–752
    [Google Scholar]
  11. Gompels U. A., Macaulay H. A. 1995; Characterization of human telomeric repeat sequences from human herpesvirus 6 and relationship to replication. Journal of General Virology 76:451–458
    [Google Scholar]
  12. Gompels U. A., Nicholas J., Lawrence G., Jones M., Thomson B. J., Martin M. E. D., Efstathiou S., Craxton M., Macaulay H. A. 1995; The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution. Virology 209:29–51
    [Google Scholar]
  13. Hidaka Y., Okada K., Kusuhara K., Miyazaki C., Tokugawa K., Ueda K. 1994; Exanthem subitum and human herpesvirus-7 infection. Pediatric Infectious Disease Journal 13:1010–1011
    [Google Scholar]
  14. Kishi M., Harada H., Takahaski M., Tanaka A., Hayashi M., Nonoyama M., Josephs S. F., Buchbinder A., Schachter F., Ablashi D. V., Wong-Staal F., Salahuddin S. Z., Gallo R. C. 1988; A repeat sequence, GGGTTA, is shared by DNA of human herpesvirus 6 and Marek’s disease virus. Journal of Virology 62:4824–4827
    [Google Scholar]
  15. Lusso P., Markham P. D., TschaeHIer E., Veronese F. D. M., Salahuddin S. Z., Ablashi D. V., Pahwa S., Krohn K., Gallo R. C. 1988; In vitro cellular tropism of human B-lymphotropic virus (human herpesvirus-6). Journal of Experimental Medicine 167:1659–1670
    [Google Scholar]
  16. Lusso P., Secchiero P., Crowley R. W., Garzino-Demo A., Bememan Z. N., Gallo R. C. 1994; CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 91:3872–3876
    [Google Scholar]
  17. Martin M. E. D., Nicholas J., Thomson B. J., Newman C., Honess R. W. 1991; Identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6. Journal of Virology 65:5381–5390
    [Google Scholar]
  18. Mukai T., Isegawa Y., Yamanishi K. 1995; Identification of the major capsid protein gene of human herpesvirus 7. Virus Research 37:55–62
    [Google Scholar]
  19. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85:2444–2448
    [Google Scholar]
  20. Portolani M., Cermelli C., Mirandola P., DiLuca D. 1995; Isolation of human herpesvirus 7 from an infant with febrile syndrome. Journal of Medical Virology 45:282–283
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  23. Secchiero P., Nicholas J., Deng H., Xiaopeng T., van Loon N., Ruvolo V. R., Berneman Z. N., Reitz M. S., Dewhurst S. 1995; Identification of human telomeric repeat motifs at the genome termini of human herpesvirus 7: structural analysis and heterogeneity. Journal of Virology 69:8041–8045
    [Google Scholar]
  24. Staden R. 1984; Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Research 12:551–567
    [Google Scholar]
  25. Staden R. 1986; The current status and portability of our sequence handling software. Nucleic Acids Research 14:217–231
    [Google Scholar]
  26. Takahashi K., Sonoda S., Higashi K., Kondo T., Takahashi H., Takahashi M., Yumanishi K. 1989; Predominant CD4 T-lymphocyte tropism of human herpesvirus 6-related virus. Journal of Virology 63:3161–3163
    [Google Scholar]
  27. Tanaka K., Kondo T., Torigoe S., Okada S., Mukai T., Yamanishi K. 1994; Human herpesvirus 7: another causal agent for roseola (exanthem subitum). Journal of Pediatrics 125:1–5
    [Google Scholar]
  28. Thomson B. J., Dewhurst S., Gray D. 1994; Structure and heterogeneity of the a sequences of human herpesvirus 6 strain variants U1102 and Z29 and identification human telomeric repeat sequences at the genomic termini. Journal of Virology 68:3007–3014
    [Google Scholar]
  29. Wyatt L. S., Frenkel N. 1992; Human herpesvirus 7 is a constitutive inhabitant of adult human saliva. Journal of Virology 66:3206–3209
    [Google Scholar]
  30. Wyatt L. S., Rodriguez W. J., Balachandran N., Frenkel N. 1991; Human herpesvirus 7: antigenic properties and prevalence in children and adults. Journal of Virology 65:6260–6265
    [Google Scholar]
  31. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata K. 1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet i:1065–1067
    [Google Scholar]
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