1887

Abstract

We describe the nucleotide sequence, transcription pattern and open reading frames (ORFs) located on HI restriction fragment 9 (0.406–0.435 map units) in the unique long segment of the pseudorabies virus (PRV) genome. The fragment contains three nested genes with a common 3′ end. The 5′ ends of the corresponding 0.9, 1.7 and 3.3 kb mRNAs have been mapped. Fragment HI-9 contains three complete ORFs, ORF1, ORF2 and ORF2.5. ORF1, which is within the 3.3 kb transcript, encodes a protein with an apparent molecular mass of 60 kDa which is homologous to the product of the herpes simplex virus type 1 UL25 gene. The 1.7 kb mRNA contains ORF2, whose product is homologous to the herpesvirus proteinases, while the 0.9 kb transcript contains ORF2.5, which probably encodes the assembly protein precursor. ORF2 was identified as the PRV proteinase gene following expression in using the product of ORF2.5 as the substrate protein.

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1996-08-01
2024-04-25
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References

  1. Addisson C., Frazer J. R., Palfreyman J. W., O’Hara M., Preston V. G. 1984; Characterization of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids. Virology 138:246–259
    [Google Scholar]
  2. Albrecht J. C., Nicolas J., Biller D., Cameron K. R., Biesinger B., Newman C., Wittmann S., Craxton M. A., Coleman M., Fleckenstein B., Honess R. W. 1992; Primer structure of the herpesvirus saimiri genome. Journal of Virology 66:5047–5058
    [Google Scholar]
  3. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffhell T. P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310:207–211
    [Google Scholar]
  4. Ben-Porat T., Kaplan A. S. 1985; Molecular biology of pseudorabies virus. In The Herpesviruses vol 3 pp 105–173 Edited by Roizman B. New York: Plenum Press;
    [Google Scholar]
  5. Burck P. J., Bergf D. H., Luk T. P., Sassmannshausen L. M., Walkulchik M., Smith D. P., Hsihng H. M., Becker G. W., Gibson W., Villarreal E. C. 1994; Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification and enzymatic characterization by using peptide substrate mimics of natural cleavage site. Journal of Virology 68:2937–2946
    [Google Scholar]
  6. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsneli T., Hutchison C. A. III, Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell K. M. 1990; Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  7. Cistue C., Tabarés E. 1992; Expression in vivo and in vitro of the major structural protein (VP73) of African swine fever virus. Archives in Virology 132:111–124
    [Google Scholar]
  8. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  9. Deatly A. M., Feldman L. T., Ben-Porat T. 1984; The large late transcripts synthesized in herpesvirus suid (pseudorabies) virus-infected cells are not precursors of mRNA. Virology 135:452–465
    [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  11. De Wind N., Peeters B. P. H., Zijderveld A., Gielkens A. L. J., Berns A. J. M., Kimman T. G. 1994; Mutagenesis and characterization of a 41-kilobase-pair region of the pseudorabies virus genome: transcription map, search for virulence genes, and comparison with homologs of herpes simplex virus type 1. Virology 200:784–790
    [Google Scholar]
  12. Gao M., Matusick-Kumar L., Hurlburt W., Ditusa S. F., Newcomb W. W., Brown J. C., McCann P. J. III, Deckman I., Colonno R. J. 1994; The protease of herpes simplex virus type I is essential for functional capsid formation and viral growth. Journal of Virology 68:3702–3712
    [Google Scholar]
  13. Griffin A. M. 1990; The complete sequence of the capsid p40 gene from infectious laryngotracheitis virus. Nucleic Acids Research 18:3664
    [Google Scholar]
  14. Kingston R. E. 1987; Primer extension. In Current Protocols in Molecular Biology pp 4.8.1.–4.8.3 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struht K. New York: John Wiley and Sons;
    [Google Scholar]
  15. Kroll D. J., Abdel-Hafiz H. A.-M., Marcel T., Simpson S., Chen C.-Y., Gutierrez-Hartmann A., Lustbader J. W., Hoeffler J. P. 1993; A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection. DNA and Cell Biology 12:441–453
    [Google Scholar]
  16. Landin B. F., Ihara S., Hampl H., Ben-Porat T. 1982; Pathway of the assembly of herpesvirus capsids: an analysis using DNA temperature sensitive mutants of pseudorabies virus. Virology 116:554–561
    [Google Scholar]
  17. Liu F., Roizman B. 1991; The promoter, transcriptional unit and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame. Journal of Virology 65:206–212
    [Google Scholar]
  18. Liu F., Roizman B. 1992; Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene. Proceedings of the National Academy of Sciences, USA 89:2076–2080
    [Google Scholar]
  19. Liu F., Roizman B. 1993; Characterization of the protease and other products of amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein. Journal of Virology 67:1300–1309
    [Google Scholar]
  20. McCann P. J. III, O’Boyle D. R. II, Deckman I. C. 1994; Investigation of the specificity of the herpes simplex type 1 protease by point mutagenesis of the proteolysis site. Journal of Virology 68:526–529
    [Google Scholar]
  21. McCracken R. M., Clarke J. K. 1971; A thin section study of the morphogenesis of Aujeszky’s disease virus in synchronously infected cell cultures. Archio fuer die Gesamte Virusforschung 34:189–201
    [Google Scholar]
  22. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  23. Martín Hernández A. M., Tabarés E. 1991; Expression and characterization of the thymidine kinase gene of African swine fever virus. Journal of Virology 65:1046–1052
    [Google Scholar]
  24. Newcomb W. W., Brown J. C. 1991; Structure of the herpes simplex virus capsid: effects of the extraction with guanidine hydrochloride and partial reconstitution of extracted capsids. Journal of Virology 65:613–620
    [Google Scholar]
  25. Preston V. G., Coates J. A. V., Rixon F. J. 1983; Identification and characterization of the herpes simplex gene product required for encapsidation of virus DNA. Journal of Virology 45:1056–1064
    [Google Scholar]
  26. Prieto J., Martín Hernández A. M., Tabarés E. 1991; Loss of pseudorabies virus thymidine kinase activity due to a single base mutation and amino acid substitution. Journal of General Virology 72:1435–1439
    [Google Scholar]
  27. Robbins A. K., Watson R. J., Whealy M. E., Hays W. W., Enquist L. W. 1986; Characterization of the pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C. Journal of Virology 58:339–347
    [Google Scholar]
  28. Robertson G. R., Whalley J. M. 1988; Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesviruses thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase. Nucleic Acids Research 16:11303–11317
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  31. Sardana V. V., Wolfgang J. A., Veloski C. A., Long W. J., Legrow K., Wolanski B., Emini E. A., Lafemina R. L. 1994; Peptide substrate cleavage specificity of the human cytomegalovirus protease. Journal of Biological Chemistry 269:14337–14340
    [Google Scholar]
  32. Steffy K. R., Schoen S., Chen C.-M. 1995; Nucleotide sequence of the herpes simplex virus type 2 gene encoding the protease and capsid protein ICP35. Journal of General Virology 76:1069–1072
    [Google Scholar]
  33. Telford E. A. R., Watson M. S., McBride K., Davison A. J. 1992; The DNA sequence of equine herpesvirus. Virology 189:304–316
    [Google Scholar]
  34. Welch A. R., McNally L. M., Gibson W. 1991a; Cytomegalovirus assembly protein nested gene family: four 3′-coterminal transcripts encode four in-frame overlapping proteins. Journal of Virology 65:4091–4100
    [Google Scholar]
  35. Welch A. R., Woods A. S., McNally L. M., Cotter R. J., Gibson W. 1991b; A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain and cleavage site. Proceedings of the National Academy of Sciences, USA 88:10792–10796
    [Google Scholar]
  36. Welch A. R., McNally L. M., Hall M. R. T., Gibson W. 1993; Herpesvirus proteinase: site-directed mutagenesis used to study maturational, release, and inactivation cleavage site of precursor and to identify a possible catalytic site serine and histidine. Journal of Virology 67:7360–7372
    [Google Scholar]
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