1887

Abstract

Western blot analysis with monoclonal antibodies, produced in response to immunization with gradient-purified adenovirus 41 (Ad41) virions, identified two epitopes of interest on protein VI of enteric adenoviruses. One epitope is unique to subgenus F adenoviruses (Ad40 and Ad41); the other epitope is common to subgenus A (Ad12, 18 and 31) and subgenus F(Ad40, 41) adenoviruses but is not shared by representative serotypes of subgenera B (Ad3 and 7), C (Ad1, 2 and 5), D (Ad8) or E (Ad4). Alignment of the deduced amino acid sequence of the genes encoding the protein VI precursor (pre-VI) of Ad40 and Ad41 (subgenus F), Ad12 and Ad31 (subgenus A), Ad2 and Ad5 (subgenus C) shows that the N-terminal one-third and C-terminal 23 amino acids of pre-VI are highly conserved. Within the central domain, pre-VI of subgenus F serotypes is more closely related to that of subgenus A serotypes than to pre-VI of the non-enteric subgenus C adenoviruses (Ad2 and Ad5). By expressing random oligonucleotide fragments of the Ad41 protein VI gene as part of a T7 gene 10 fusion protein, the two epitopes of interest were mapped to within the same 14 amino acid region in the central domain of protein VI. Given the association of subgenera A and F adenoviruses with paediatric gastroenteritis, the epitope shared by these serotypes may be functionally significant with respect to gut tropism. In addition, this epitope is potentially valuable as a target for the detection of enteric adenoviruses in clinical specimens.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-8-1811
1996-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/8/JV0770081811.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-8-1811&mimeType=html&fmt=ahah

References

  1. Ahluwalia G. S., Scott-Taylor T. H., Klisko B., Hammond G. W. 1995; Comparison of detection methods for adenovirus from enteric clinical specimens. Diagnostic Microbiology and Infectious Disease 18:161–166
    [Google Scholar]
  2. Bailey A., Mautner V. 1994; Phylogenetic relationships among adenovirus serotypes. Virology 205:438–452
    [Google Scholar]
  3. Bailey A. C., MacKay N., Mautner V. 1993; Enteric adenovirus type 40: expression of E1B proteins in vitro and in vivo . Virology 193:631–641
    [Google Scholar]
  4. Bailey A., Ullah R., Mautner V. 1994; Cell type specific regulation of expression from the Ad40 E1B promoter in recombinant Ad5/Ad40 viruses. Virology 202:695–706
    [Google Scholar]
  5. Brown M. 1990; Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986. Journal of Clinical Microbiology 28:1525–1529
    [Google Scholar]
  6. Brown M., Petrie M., Middleton P. J. 1984; Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens. Journal of Clinical Microbiology 20:334–338
    [Google Scholar]
  7. Brown M., Wilson-Friesen H. L., Doane F. 1992; A block in release of progeny virus and a high particle-to-infectious unit ratio contribute to poor growth of enteric adenovirus types 40 and 41 in cell culture. Journal of Virology 66:3198–3205
    [Google Scholar]
  8. Chroboczek J., Bieber F. R., Jacrot B. 1992; The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2. Virology 186:280–285
    [Google Scholar]
  9. Cruz J. R., Caceres P., Cano F., Flores J., Bartlett A., Torun B. 1990; Adenovirus types 40 and 41 and rotaviruses associated with diarrhea in children from Guatemala. journal of Clinical Microbiology 28:1780–1784
    [Google Scholar]
  10. Davison A. J., Telford E. A., Watson M. S., McBride K., Mautner V. 1993; The DNA sequence of adenovirus type 40. Journal of Molecular Biology 234:1308–1316
    [Google Scholar]
  11. De Jong J. C., Bijlsma K., Wermenbol A. G., Verweij-Uijterwaal M. W., Van Der Avoort H. G. A. M., Wood D. J., Bailey A. S., Osterhaus A. D. M. E. 1993; Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes. Journal of Clinical Microbiology 31:1562–1569
    [Google Scholar]
  12. Dunn S. D. 1986; Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies. Analytical Biochemistry 157:144–153
    [Google Scholar]
  13. Fortsas E., Petrie M., Brown M. 1994; Electrophoretic migration of adenovirus hexon under non-denaturing conditions. Virus Research 31:229–233
    [Google Scholar]
  14. Freimuth P., Anderson C. W. 1993; Human adenovirus serotype 12 virion precursors pMu and pVI are cleaved at amino-terminal and carboxy-terminal sites that conform to the adenovirus 2 endoproteinase cleavage consensus sequence. Virology 193:348–355
    [Google Scholar]
  15. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  16. Grimwood K., Carzino R., Barnes J. L., Bishop R. F. 1995; Patients with enteric adenovirus gastroenteritis admitted to an Australian pediatric teaching hospital from 1981 to 1992. Journal of Clinical Microbiology 33:131–136
    [Google Scholar]
  17. Harlow E., Lane D. 1988 Antibodies – A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Herrmann J. E., Perron-Henry D. M., Blacklow N. R. 1987; Antigen detection with monoclonal antibodies for the diagnosis of adenovirus gastroenteritis. Journal of Infectious Diseases 155:1167–1171
    [Google Scholar]
  19. Hierholzer J. C. 1992; Adenoviruses in the immunocompromised host. Clinical Microbiology Reviews 5:262–274
    [Google Scholar]
  20. Jarecki-Khan K., Tsipori S. R., Unicomb L. E. 1993; Enteric adenovirus infection among infants with diarrhea in rural Bangladesh. Journal of Clinical Microbiology 31:484–489
    [Google Scholar]
  21. Johansson M. E., Brown M., Hierholzer J. C., Thorner A., Ushijma H., Wadell G. 1991; Genome analysis of adenovirus type 31 strains from immunocompromised and immunocompetent patients. Journal of Infectious Diseases 163:293–299
    [Google Scholar]
  22. Johansson M. E., Andersson M. A., Thorner P. A. 1994; Adenoviruses isolated in the Stockholm area during 1987–1992: restriction endonuclease analysis and molecular epidemiology. Archives of Virology 137:101–115
    [Google Scholar]
  23. Keefe L. J., Ginell S. L., Westbrook E. M., Anderson C. W. 1995; Crystallization and preliminary X-ray diffraction studies of the human adenovirus serotype 2 proteinase with peptide cofactor. Protein Science 4:1658–1660
    [Google Scholar]
  24. Kidd A. H., Garwicz D., Oberg M. 1995; Human and simian adenoviruses: Phylogenetic inferences from analysis of VA RNA genes. Virology 207:32–45
    [Google Scholar]
  25. Lew J. F., Moe C. L., Monroe S. S., Allen J. R., Harrison B. M., Forrester B. D., Stine S. E., Woods P. A., Hierholzer J. C., Herrmann J. E., Blacklow N. R., Bartlett A. V., Glass R. I. 1991; Astrovirus and adenovirus associated with diarrhea in children in day care settings. Journal of Infectious Diseases 164:673–678
    [Google Scholar]
  26. Mangel W. F., McGrath W. J., Toledo D. L., Anderson C. W. 1993; Viral DNA and a viral peptide can act as cofactors of adenovirus virion proteinase activity. Nature 361:274–275
    [Google Scholar]
  27. Matthews D. A., Russell W. C. 1994; Adenovirus protein-protein interactions: hexon and protein VI. Journal of General Virology 75:3365–3374
    [Google Scholar]
  28. Matthews D. A., Russell W. C. 1995; Adenovirus protein–protein interactions: molecular parameters governing the binding of protein VI to hexon and the activation of the adenovirus 23K protease. Journal of General Virology 76:1959–1969
    [Google Scholar]
  29. Mautner V., MacKay N., Steinthorsdottir V. 1989; Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12. Virology 171:619–622
    [Google Scholar]
  30. Mautner V., Steinthorsdottir V., Bailey A. 1995; Enteric adenoviruses. In The Molecular Repertoire of Adenoviruses Edited by Doerfler W., Bohm P. Heidelberg: Springer-Verlag;
    [Google Scholar]
  31. Mickan L. D., Kok T.-W. 1994; Recognition of adenovirus types in faecal samples by Southern hybridization in South Australia. Epidemiology and Infection 112:603–613
    [Google Scholar]
  32. Noel J., Mansoor A., Thaker U., Herrmann J., Perron-Henry D., Cubitt W. D. 1994; Identification of adenoviruses in faeces from patients with diarrhoea at the Hospitals for Sick Children, London, 1989–1992. Journal of Medical Virology 43:84–90
    [Google Scholar]
  33. Roberts R. J., Akusjarvi G., Alestrom P., Gelinas R. E., Gingeras T. R., Sciaky D., Pettersson U. 1996; A consensus sequence for the adenovirus-2 genome. In Adenovirus DNA – The Viral Genome and its Expression Edited by Doerfler W. Boston: Martinus Nijhoff;
    [Google Scholar]
  34. Rodriguez F. H., Liuzza G. E., Gohd R. H. 1984; Disseminated adenovirus 31 infection in an immunocompromised host. American Journal of Clinical Pathology 82:615–618
    [Google Scholar]
  35. Russell W. C., Precious B. 1982; Nucleic acid-binding properties of adenovirus structural polypeptides. Journal of General Virology 63:69–79
    [Google Scholar]
  36. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning - A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Schnurr D., Bollen A., Crawford-Miksza L., Dondero M. E., Yagi S. 1995; Adenovirus mixture isolated from the brain of an AIDS patient with encephalitis. Journal of Medical Virology 47:168–171
    [Google Scholar]
  38. Schofield K. P., Morris D. J., Bailey A. S., de Jong J. C., Corbitt G. 1994; Gastroenteritis due to adenovirus type 41 in an adult with chronic lymphocytic leukemia. Clinical Infectious Diseases 19:311–312
    [Google Scholar]
  39. Scott-Taylor T. H., Hammond G. W. 1995; Local succession of adenovirus strains in pediatric gastroenteritis. Journal of Medical Virology 45:331–338
    [Google Scholar]
  40. Scott-Taylor T. H., Ahluwalia G., Klisko B., Hammond G. W. 1990; Prevalent adenovirus variant not detected by commercial monoclonal antibody enzyme immunoassay. Journal of Clinical Microbiology 28:2797–2801
    [Google Scholar]
  41. Song B., Spindler K., Young C. S. H. 1995; Sequence of the mouse adenovirus serotype-1 DNA encoding the precursor to capsid protein VI. Gene 152:279–280
    [Google Scholar]
  42. Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W. 1994; Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis. Journal of Virology 68:379–389
    [Google Scholar]
  43. Stewart P. L., Fuller S. D., Burnett R. M. 1993; Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO Journal 12:2589–2593
    [Google Scholar]
  44. Tiemessen C. T., Kidd A. H. 1994; Adenovirus type 40 and 41 growth in vitro: host range diversity reflected by differences in patterns of DNA replication. Journal of Virology 68:1239–1244
    [Google Scholar]
  45. Tiemessen C. T., Kidd A. H. 1995; The subgroup F adenoviruses. Journal of General Virology 76:481–497
    [Google Scholar]
  46. Van R., Wun C. C., O’Ryan M., Matson D. O., Jackson L., Pickering L. K. 1992; Outbreaks of human enteric adenovirus types 40 and 41 in Houston day care centers. Journal of Pediatrics 120:516–521
    [Google Scholar]
  47. van der Avoort H. G. A. M., Wermenbol A. G., Zomerdijk T. P. L., Kleijne J. A. F. W., van Asten J. A. A. M., Jensma P., Osterhaus A. D. M. E., Kidd A. H., de Jong J. C. 1989; Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies. Virus Research 12:139–158
    [Google Scholar]
  48. Webster A., Hay R. T., Kemp G. 1993; The adenovirus protease is activated by a virus-coded disulphide-linked peptide. Cell 72:97–104
    [Google Scholar]
  49. Webster A., Russell S., Talbot P., Russell W. C., Kemp G. D. 1989; Characterization of the adenovirus proteinase: substrate specificity. Journal of General Virology 70:3225–3234
    [Google Scholar]
  50. Wood D. J., Bijlsma K., de Jong J. C., Tonkin C. 1989; Evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens. Journal of Clinical Microbiology 27:1155–1158
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-8-1811
Loading
/content/journal/jgv/10.1099/0022-1317-77-8-1811
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error