%0 Journal Article %A Marriott, Anthony C. %A Nuttall, Patricia A. %T Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase %D 1996 %J Journal of General Virology, %V 77 %N 8 %P 1775-1780 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-77-8-1775 %I Microbiology Society, %X The nucleotide sequence of the large (L) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) was determined, completing the first entire genome sequence of a nairovirus. The L segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (M) and small (S) genomic segments. A single open reading frame (ORF) was present in the viral complementary strand, sufficient to encode a protein of 459 kDa. The predicted protein sequence showed the core polymerase motifs characteristic of the RNA-dependent RNA polymerases of segmented negative-stranded viruses. Comparison of the conserved motifs with the corresponding region of other segmented negative-strand viruses showed a closer relationship between nairoviruses and phleboviruses than with other Bunyaviridae or with other virus families. However, the core polymerase was the only function that could be assigned to a region of the DUG L gene. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-8-1775