1887

Abstract

The nucleotide sequence of the large (L) RNA segment of Dugbe (DUG) virus () was determined, completing the first entire genome sequence of a nairovirus. The L segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (M) and small (S) genomic segments. A single open reading frame (ORF) was present in the viral complementary strand, sufficient to encode a protein of 459 kDa. The predicted protein sequence showed the core polymerase motifs characteristic of the RNA-dependent RNA polymerases of segmented negative-stranded viruses. Comparison of the conserved motifs with the corresponding region of other segmented negative-strand viruses showed a closer relationship between nairoviruses and phleboviruses than with other or with other virus families. However, the core polymerase was the only function that could be assigned to a region of the DUG L gene.

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/content/journal/jgv/10.1099/0022-1317-77-8-1775
1996-08-01
2021-10-25
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References

  1. de Haan P., Kormelink R., Resende R. DE O., van Poelwijk F., Peters D., Goldbach R. 1991; Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. Journal of General Virology 72:2207–2216
    [Google Scholar]
  2. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  3. Elliott R. M. 1990; Molecular biology of the Bunyaviridae . Journal of General Virology 71:501–522
    [Google Scholar]
  4. Felsenstein J. 1993 PHYLIP (Phylogeny Inference Package), version 3.5c Department of Genetics. University of Washington; Seattle, Wash., USA.:
    [Google Scholar]
  5. Hetzer M., Mueller M. W. 1993; PCR mediated analysis of RNA sequences. Nucleic Acids Research 21:5526–5527
    [Google Scholar]
  6. Jin H., Elliott R. M. 1991; Expression of functional Bunyamwera virus L protein by recombinant vaccinia viruses. Journal of Virology 65:4182–4189
    [Google Scholar]
  7. Jin H., Elliott R. M. 1992; Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies. Journal of General Virology 73:2235–2244
    [Google Scholar]
  8. Jin H., Elliott R. M. 1993; Non-viral sequences at the 5′ ends of Dugbe nairovirus S mRNAs. Journal of General Virology 74:2293–2297
    [Google Scholar]
  9. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 1993 MEGA: Molecular Evolutionary Genetics Analysis, version 1.01. Pennsylvania State University, University Park, PA 16802; USA.:
    [Google Scholar]
  11. Marriott A. C., El-Ghorr A. A., Nuttall P. A. 1992; Dugbe nairovirus M RNA: nucleotide sequence and coding strategy. Virology 190:606–615
    [Google Scholar]
  12. Muller R., Poch O., Delarue M., Bishop D. H. L., Bouloy M. 1994; Rift valley fever virus L segment: correction of the sequence and possible functional role of newly identified regions conserved in RNA-dependent polymerases. Journal of General Virology 75:1345–1352
    [Google Scholar]
  13. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. (editors) 1995 Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses pp 300–315 Vienna & New York: Springer-Verlag;
    [Google Scholar]
  14. Poch O., Sauvaget I., Delarue M., Tordo N. 1989; Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO Journal 8:3867–3874
    [Google Scholar]
  15. Roberts A., Rossier C., Kolakofsky D., Nathanson N., Gonzalez-Scarano F. 1995; Completion of the La Crosse virus genome sequence and genetic comparisons of the L proteins of the Bunyaviridae . Virology 206:742–745
    [Google Scholar]
  16. Ward V. K., Marriott A. C., El-Ghorr A. A., Nuttall P. A. 1990; Coding strategy of the S RNA segment of Dugbe virus (Nairovirus; Bunyaviridae). Virology 175:518–524
    [Google Scholar]
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