1887

Abstract

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp108, has been identified by two different panels of monoclonal antibodies. The gII- and gp108-specific monoclonal antibodies were shown to react with the same protein, which was identified by N-terminal sequencing as the homologue of herpes simplex virus type 1 (HSV-1) gH. When BHV-1 gH was purified by immunoadsorbent chromatography, gL was co-purified. The gH-gL complex induced the production of antibodies that neutralized virus infectivity and inhibited virus penetration. Affinity-purified gH-gL did prevent penetration, but not attachment of BHV-1, which suggests that the gH-gL complex is essential for penetration of BHV-1 into susceptible cells.

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1996-07-01
2024-12-12
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References

  1. Baranowski E., Dubuisson J., Pastoret P.-P., Thiry E. 1993; Identification of 108k, 93k, and 42k glycoproteins of bovine herpesvirus-1 by monoclonal antibodies. Archives of Virology 133:97–111
    [Google Scholar]
  2. Browne H., Baxter V., Minson A. 1993; Analysis of protective immune responses to the glycoprotein H-glycoprotein L complex of herpes simplex virus type I. Journal of General Virology 74:2813–2817
    [Google Scholar]
  3. Cranage M. P., Smith G., Bell S., Hart H., Brown C., Bankier A., Tomlinson T., Bareli B., Minson A. 1988; Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein–Barr virus BXLF2 product, varicella zoster virus gpIII and herpes simplex virus type 1 glycoprotein H. Journal of Virology 62:1416–1422
    [Google Scholar]
  4. Fuller A. O., Santos R. E., Spear P. G. 1989; Neutralizing antibodies specific for glycoprotein H of herpes simplex virus permit viral attachment to cells but prevent penetration. Journal of Virology 63:3435–3443
    [Google Scholar]
  5. Highlander S. L., Sutherland S. L., Gage P. J., Johnson D. C., Levine M., Glorioso J. C. 1987; Neutralizing antibodies specific for herpes simplex virus glycoprotein D inhibit virus penetration. Journal of Virology 61:3356–3364
    [Google Scholar]
  6. Hughes G., Babiuk L. A., Van Drunen Little-van den Hurk S. 1988; Functional and topographical analyses of epitopes on bovine herpesvirus-I glycoprotein IV. Archives of Virology 103:47–60
    [Google Scholar]
  7. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K., Minson A., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein gH and affects normal folding and surface expression of gH. Journal of Virology 66:2240–2250
    [Google Scholar]
  8. Johnson D. C., Burke R. L., Gregory T. 1990; Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells. Journal of Virology 64:2569–2576
    [Google Scholar]
  9. Kaye J. F., Gompels U. A., Minson A. C. 1992; Glycoprotein gH of human cytomegalovirus (HCMV) forms a stable complex with the HCMV UL115 gene product. Journal of General Virology 73:2693–2698
    [Google Scholar]
  10. Khattar S., Van Drunen Little-van den Hurk S., Babiuk L. A., Tikoo S. 1995; Identification and transcriptional analysis of a 3′-coterminal gene cluster containing UL1, UL2, UL3, and UL3.5 open reading frames of bovine herpesvirus–I. Virology 213:28–37
    [Google Scholar]
  11. Klupp B. G., Mettenleiter T. C. 1991; Sequence and expression of the glycoprotein gH gene of pseudorabies virus. Virology 182:732–741
    [Google Scholar]
  12. Klupp B. G., Baumeister J., Karger A., Visser N., Mettenleiter T.C. 1994; Identification and characterization of a novel structural glycoprotein in pseudorabies virus, gL. Journal of Virology 68:3868–3878
    [Google Scholar]
  13. Liang X., Babiuk L. A., Van Drunen Little-van den Hurk S., Fitzpatrick D. R., Zamb T. J. 1991; Bovine herpesvirus-1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII, and gIV. Journal of Virology 65:1124–1132
    [Google Scholar]
  14. Liu D. X., Gompels U. A., Foa-Tomasi L., Campadelli-Fiume G. 1993; Human herpesvirus-6 glycoprotein H and L homologs are components of the gp100 complex and the gH external domain is the target for neutralizing monoclonal antibodies. Virology 197:12–22
    [Google Scholar]
  15. McGeoch D., Davidson A. 1986; DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein H, and identification of homologues in the genome of varicella zoster virus and Epstein–Barr virus. Nucleic Acids Research 14:4281–4292
    [Google Scholar]
  16. Meyer A. L., Petrovskis E. A., Duffus W. P. H., Thomsen D. R., Post L. E. 1991; Cloning and sequencing of an infectious bovine rhinotracheitis virus (BHV-1) gene homologous to glycoprotein H of herpes simplex virus. Biochemica et Biophysica Acta 1090:267–269
    [Google Scholar]
  17. Nicolson L., Cullinane A., Onions D. 1990; The nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus type 1 glycoprotein H gene. Journal of General Virology 71:1793–1800
    [Google Scholar]
  18. Van Drunen Little-van den Hurk S., Babiuk L. A. 1985; Antigenic and immunogenic characteristics of bovine herpesvirus type-1 glycoproteins GVP 3/9 and GVP 6/11/16, purified by immunoadsorbent chromatography. Virology 144:204–215
    [Google Scholar]
  19. Van Drunen Little-van den Hurk S., Babiuk L. A. 1986; Synthesis and processing of bovine herpesvirus-1 glycoproteins. Journal of Virology 59:401–410
    [Google Scholar]
  20. Yaswen L. R., Campbell C., Devenport L. C., Stephens E. B., Hutt-Fletcher L. M. 1992; Expression and analysis of the Epstein–Barr virus gH. Abstracts of the 17th International Herpesvirus Workshop abstract 273
    [Google Scholar]
  21. Yates W. D. G. 1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral–bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine 46:225–263
    [Google Scholar]
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