We have recently shown that the Semliki Forest virus (SFV) subgenome contains a translation enhancer region in the coding part of its RNA. The enhancer increases the translation of the viral structural genes approximately ten-fold and thereby plays a key role in virus assembly. In this study we conclusively show that this translation enhancer represents an adaptation to the
Berben-BloemenheuvelG.,
KasperaitisM. A. M.,
van HeugtenH.,
ThomasA. A. M.,
van SteegH.,
VoormaH. O.1992; Interaction of initiation factors with the cap structure of chimaeric mRNA containing the 5′-untranslated regions of Semliki Forest virus RNA is related to translational efficiency. European Journal of Biochemistry 208:581–587
FrolovI.,
SchlesingerS.1994; Translation of Sindbis virus mRNA: effects of sequences downstream of the initiation codon. Journal of Virology 68:8111–8117
GarryR. F.1994; Sindbis virus-induced inhibition of protein synthesis is partially reversed by medium containing an elevated potassium concentration. Journal of General Virology 75:411–415
GarryR. F.,
BishopJ. M.,
ParkerS.,
WestbrookK.,
LewisG.,
WaiteM. R. F.1979; Na+ and K+ concentrations and the regulation of protein synthesis in Sindbis-virus infected chick cells. Virology 96:108–120
KozakM.1994; Features in the 5′ non-coding sequences of rabbit (α- and β;-globin mRNAs that affect translational efficiency. Journal of Molecular Biology 235:95–110
LiljestromP.,
LusaS.,
HuylebroeckD.,
GaroffH.1991; In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the 6, 000-molecular-weight membrane protein modulates virus release. Journal of Virology 65:4107–4113
SanesJ.,
RubensteinJ. L. R.,
NicolasJ.-F.1986; Use of recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO Journal 5:3133–3142
SjöbergM.,
SuomalainenM.,
GaroffH.1994; A significantly improved Semliki Forest virus expression system based on translation enhancer segments from the viral capsid gene. Biotechnology 12:1127–1131
StraussE. G.,
StraussJ. H.1986; Structure and replication of the alphavirus genome. In The Togaviridae and Flaviviridae Edited by
SchlesingerJ.,
SchlesingerS.
New York: Plenum Press;
SuomalainenM.,
BaronM.,
GaroffH. Y.1990; The E2 signal sequence of Rubella virus remains part of the capsid protein and confers membrane association in vitro. Journal of Virology 64:5500–5509
SuomalainenM.,
GaroffH.1994; Incorporation of homologous and heterologous proteins into the envelope of moloney leukemia virus. Journal of Virology 68:4879–4889
WahlbergJ. M.,
BoereW. A.,
GaroffH.1989; The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to mildly acidic pH during virus maturation. Journal of Virology 63:4991–4997