1887

Abstract

The subgenomic mRNAs (sg mRNA) of porcine reproductive and respiratory syndrome virus (PRRSV) were characterized. The number of sg mRNAs, which form a 3′-coterminal nested set in PRRSV-infected cells, varied from six to seven among PRRSV isolates with differing virulence. The additional species of sg mRNA in some isolates of PRRSV was designated as sg mRNA 3-1. The leader-mRNA junction sequences of sg mRNAs 3, 3-1 and 4 were found to contain a similar six nucleotide sequence motif, U(G)UA(G/C)ACC. By comparing the 5′-terminal sequence of sg mRNA 3-1 with the genomic sequence of two isolates, ISU79 and ISU1894, it was found that a point mutation, from U in isolate ISU1894 to C in isolate ISU79, led to the acquisition of a new leader-mRNA junction sequence (UUGACC) in isolate ISU79, and therefore an additional species of sg mRNA 3-1. A small ORF (3-1) was identified at the 5′ end of sg mRNA 3-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-6-1265
1996-06-01
2024-11-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/6/JV0770061265.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-6-1265&mimeType=html&fmt=ahah

References

  1. Cavanagh D., Brian D. A., Enjuanes L., Holmes K. V., Lai M. M. C., Laude H., Siddell S. G., Spaan W., Taguchi F., Talbot P. J. 1990; Recommendations of the coronavirus study group for the nomenclature of the structural proteins, mRNAs, and genes of coronaviruses. Virology 176:306–307
    [Google Scholar]
  2. Chen Z., Kuo L., Rowland R. R. R., Even C., Faberge K. S., Plagemann P. G. W. 1993; Sequences of 3′ end of genome and of 5′ end of open reading frame la of lactate dehydrogenase-elevating virus and common junction motifs between 5′ leader and bodies of seven subgenomic mRNAs. Journal of General Virology 74:643–660
    [Google Scholar]
  3. Collins J. E., Benfield D. A., Christianson W. J., Harris L., Hennings J. C., Shaw D. P., Goyal S. M., McCullough S., Morrison R. B., Joo H. S., Gorcyca D. E., Chladek D. W. 1992; Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North American and experimental reproduction of the disease in gnotobiotic pigs. Journal of Veterinary Diagnostic Investigation 4:117–126
    [Google Scholar]
  4. Conzelmann K.-K., Visser N., Van Woensel P., Thiel H.-J. 1993; Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the Arterivirus group. Virology 193:329–339
    [Google Scholar]
  5. De Vries A. A. F., Chirnside E. D., Bredenbeek P. J., Gravestein L. A., Horzinek M. C., Spaan W. J. M. 1990; All subgenomic mRNAs of equine arteritis virus contain a common leader sequence. Nucleic Acids Research 18:3241–3247
    [Google Scholar]
  6. Godeny E. K., Zeng L., Smith S. L., Brinton M. A. 1995; Molecular characterization of the 3′ terminus of the simian hemorrhagic fever virus genome. Journal of Virology 69:2679–2683
    [Google Scholar]
  7. Halbur P. G., Paul P. S., Frey M. L., Landgraf J., Eernisse K., Meng X.-J., Lum M. A., Rathje J. A. 1995; Comparison of the pathogenicity of two U,. S. porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Veterinary Pathology 32:648–660
    [Google Scholar]
  8. Halbur P. G., Paul P. S., Meng X.-J., Lum M. A., Rathje J. A. 1996; Comparative pathogenicity of nine U,. S. porcine reproductive and respiratory syndrome virus (PRRSV) isolates in a 5 week-old cesarean-derived-colostrum-deprived pig model. Journal of Veterinary Diagnostic Investigation 8: in press
    [Google Scholar]
  9. Kuo L., Harty J. T., Erickson L., Palmer G. A., Plagemann P. G. W. 1991; A nested set of eight RNAs is formed in macrophages infected with lactate dehydrogenase-elevating virus. Journal of Virology 65:5118–5123
    [Google Scholar]
  10. Kuo L., Chen Z., Rowland R. R. R., Faberge K. S., Plagemann P. G. W. 1992; Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3′-terminal sequences of two LDV isolates. Virus Research 23:55–72
    [Google Scholar]
  11. Meng X.-J., Paul P. S., Halbur P. G. 1994; Molecular cloning and nucleotide sequencing of the 3′-terminal genomic RNA of the porcine reproductive and respiratory syndrome virus. Journal of General Virology 75:1795–1801
    [Google Scholar]
  12. Meng X.-J., Paul P. S., Halbur P. G., Lum M. A. 1995a; Phylogenetic analysis of the putative M (ORF6) and N (ORF7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the U,. S. and Europe. Archives of Virology 140:745–755
    [Google Scholar]
  13. Meng X.-J., Paul P. S., Halbur P. G., Morozov I. 1995b; Sequence comparison of the open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus (PRRSV). Journal of General Virology 76:3181–3188
    [Google Scholar]
  14. Meulenberg J. J. M., Hulst M. M., De Meijer E. J., Moonen P. L. J. M., Den Besten A., De Kluyver E. P., Wensvoort G., Moormann R. J. M. 1993a; Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72
    [Google Scholar]
  15. Meulenberg J. J. M., De Meijer E. J., Moormann R. J. M. 1993b; Subgenomic RNAs of Lelystad virus contain a conserved leader-body junction sequence. Journal of General Virology 74:1697–1701
    [Google Scholar]
  16. Morozov L., Meng X. J., Paul P. S. 1995; Sequence analysis of the open reading frames (ORF) 2 to 4 of a U,. S. isolate of porcine reproductive and respiratory syndrome virus (PRRSV). Archives of Virology 141:1313–1319
    [Google Scholar]
  17. Plagemann P. G. W., Moennig V. 1992; Lactate dehydrogenaseelevating virus, equine arteritis virus and simian hemorrhagic fever virus, a new group of positive strand RNA virus. Advances in Virus Research 41:99–192
    [Google Scholar]
  18. Sethna P. B., Hofmann M. A., Brian D. A. 1991; Minus-strand copies of replicating coronavirus mRNAs contain antileaders. Journal of Virology 65:320–325
    [Google Scholar]
  19. Wensvoort G., Terpstra C., Pol J. M., Ter Laak E. A., Bloemraad M., De Kluyver E. P., Kragten C., Van Butten L., Den Besten A., Wagenaar F., Broekhuijsen J. M., Moonen P. L. J. M., Zetstra T., De Boer E. A., Tibben H. J., De Jong M. F., Van’t Veld P., Groenland G. J. R., Van Gennep J. A., Voets M. T., Verheijden J. H. M., Braamskamp J. 1991; Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly 13:121–130
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-77-6-1265
Loading
/content/journal/jgv/10.1099/0022-1317-77-6-1265
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error