@article{mbs:/content/journal/jgv/10.1099/0022-1317-77-6-1159, author = "Contreras-Brodin, Bertha and Karlsson, Ann and Nilsson, Tina and Rymo, Lars and Klein, George", title = "B cell-specific activation of the Epstein—Barr virus-encoded C promoter compared with the wide-range activation of the W promoter", journal= "Journal of General Virology", year = "1996", volume = "77", number = "6", pages = "1159-1162", doi = "https://doi.org/10.1099/0022-1317-77-6-1159", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-77-6-1159", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We have studied the activity of reporter plasmids, carrying the Epstein—Barr virus (EBV) nuclear antigen (EBNA) promoters Wp and Cp, in a group of somatic cell hybrids obtained by fusing EBV-positive lympho-blastoid cell lines or group II/III Burkitt’s lymphoma cell lines with non-B cell lines. In B/non-B cell hybrids of this type, B cell markers are extinguished as a rule, in parallel with the inactivation of Cp or Wp and down-regulation of EBNA-2-6 expression. A Wp-carrying reporter construct was active in non-B cell lines. Only cells with a B cell phenotype could support the activity of Cp-carrying plasmids. EBNA-2 transactivated Cp only in B cells. Our data suggest that while Wp can be used for EBNA transcription in B and non-B cells, Cp activity is restricted to B cells. The inability of EBNA-2 to transactivate Cp in non-B cells indicates that other factors present in B cells might be involved in Cp transactivation.", }