1887

Abstract

We have studied the activity of reporter plasmids, carrying the Epstein—Barr virus (EBV) nuclear antigen (EBNA) promoters Wp and Cp, in a group of somatic cell hybrids obtained by fusing EBV-positive lympho-blastoid cell lines or group II/III Burkitt’s lymphoma cell lines with non-B cell lines. In B/non-B cell hybrids of this type, B cell markers are extinguished as a rule, in parallel with the inactivation of Cp or Wp and down-regulation of EBNA-2-6 expression. A Wp-carrying reporter construct was active in non-B cell lines. Only cells with a B cell phenotype could support the activity of Cp-carrying plasmids. EBNA-2 transactivated Cp only in B cells. Our data suggest that while Wp can be used for EBNA transcription in B and non-B cells, Cp activity is restricted to B cells. The inability of EBNA-2 to transactivate Cp in non-B cells indicates that other factors present in B cells might be involved in Cp transactivation.

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1996-06-01
2024-10-14
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References

  1. Altiok E., Minarovits J., Hu L. F., Contreras-Brodin B., Klein G., Ernberg I. 1992; Host cell phenotype dependent control of the BCR2-BWR1 promoter complex regulates the Epstein-Barr virus nuclear antigens 2-6. Proceedings of the National Academy of Sciences, USA 89:905–909
    [Google Scholar]
  2. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrel B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310:207–211
    [Google Scholar]
  3. Bodescot M., Perricaudet M., Farrell P. J. 1987; A promoter for the highly spliced EBNA family of RNAs of Epstein-Barr virus. Journal of Virology 61:3424–3430
    [Google Scholar]
  4. Contreras-Brodin B., Anvret M., Imreh S., Altiok E., Klein G., Masucci M. 1991; B cell phenotype-dependent expression of the Epstein-Barr virus nuclear antigens EBNA-2 to EBNA-6: studies with somatic cell hybrids. Journal of General Virology 72:3025–3033
    [Google Scholar]
  5. Ernberg I., Falk K., Minarovits J., Busson P., Tursz T., Masucci M. G., Klein G. 1989; The role of methylation in the phenotype-dependent modulation of Epstein-Barr nuclear antigen 2 and latent membrane protein genes in cells latently infected with Epstein-Barr virus. Journal of General Virology 70:2989–3002
    [Google Scholar]
  6. Henkel T., Ling P. D., Hayward S. D., Peterson M. G. 1994; Mediation of Epstein-Barr virus EBNA-2 transactivation by recombination signal-binding protein J Kappa. Science 169:188–190
    [Google Scholar]
  7. Jin X. W., Speck S. 1992; Identification of critical cis elements involved in mediating Epstein-Barr virus nuclear antigen 2-dependent activity of an enhancer located upstream of the viral BamHI C promoter. Journal of Virology 66:2846–2852
    [Google Scholar]
  8. Kieff E., Wang F., Birkenbach M., Cohen J., Sample J., Tomkinson B., Swaminathan S., Longnecker R., Marchini A., Mannick J., Tsang S., Sample C., Kurilla M. 1991; Molecular biology of lymphocyte transformation by Epstein-Barr virus. In Origins of Human Cancer: A Comprehensive Review pp. 563–576 Edited by Brigge J., Curran T., Harlow E., McCormack F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Ling P. D., Rawlins D. R., Hayward S. D. 1993; The Epstein-Barr virus immortalizing protein EBNA-2 is targeted to DNA by a cellular enhancer binding protein. Proceedings of the National Academy of Sciences, USA 90:9237–9241
    [Google Scholar]
  10. Nilsson T., Sjöblom A., Masucci M. G., Rymo L. 1993; Viral and cellular factors influence the activity of the Epstein-Barr virus BCR2 and BWR1 promoters in cells of different phenotype. Virology 193:774–785
    [Google Scholar]
  11. Ricksten A., Olsson A., Andersson T., Rymo L. 1988; The 5′ flanking region of the gene for the Epsein-Barr virus-encoded nuclear antigen 2 contains a cell type specific m-acting regulatory element that activates transcription in transfected B cells. Nucleic Acids Research 16:8391–8410
    [Google Scholar]
  12. Robertson E., Grossman S., Johanssen E., Miller C., Lin J., Tomkinson B., Kieff E. 1995; Epstein-Barr virus nuclear protein 3C modulates transcription through interaction with the sequence specific DNA-binding protein Jĸ . Journal of Virology 69:3108–3116
    [Google Scholar]
  13. Rooney C., Brimmell M., Buschle M., Gordon A., Farrell P. J., Kolman J. 1992; Host cell and EBNA-2 regulation of Epstein-Barr virus latent cycle promoter activity in B-lymphocytes. Journal of Virology 66:496–504
    [Google Scholar]
  14. Rowe D. T., Rowe M., Evan G. L., Wallace L. E., Farrell P. J., Rickinson A. B. 1986; Restricted expression of EBV latent genes and T-lymphocyte detected membrane antigen in Burkitt lymphoma cells. EMBO Journal 5:2599–2607
    [Google Scholar]
  15. Sample J., Hummel M., Braun D., Birkenbach M., Kieff E. 1986; Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins. A probable transcriptional initiation site. Proceedings of the National Academy of Sciences, USA 83:5096–5100
    [Google Scholar]
  16. Sample J., Brooks L., Sample C., Young L., Rowe M., Gregory C., Rickinson A., Kieff E. 1991; Restricted Epstein-Barr virus protein expression in Burkitt lymphoma is due to a different Epstein-Barr nuclear antigen 1 transcriptional activation site. Proceedings of the National Academy of Sciences USA 88:6343–6347
    [Google Scholar]
  17. Speck S. H., Pfitzner A. J., Strominger J. L. 1986; An Epstein-Barr virus transcript from a latently infected growth transformed B-cell line encodes a highly repetitive polypeptide. Proceedings of the National Academy of Sciences, USA 83:9298–9302
    [Google Scholar]
  18. Sung N. S., Kenney S., Gutsch D., Pagano J. S. 1991; EBNA-2 transactivates a lymphoid specific enhancer in the Bam HI C promoter of Epstein-Barr virus. Journal of Virology 65:2164–2169
    [Google Scholar]
  19. Sugden B., Warren N. 1989; A promoter of Epstein-Barr virus that can function during latent infection can be transactivated by EBNA-1; a viral protein required for viral DNA replication during latent infection. Journal of Virology 63:2664–2649
    [Google Scholar]
  20. Woisetschlaeger M., Strominger J. L., Speck S. H. 1989; Mutually exclusive use of viral promoters in Epstein-Barr virus latently infected lymphocytes. Proceedings of the National Academy of Sciences, USA 86:6498–6502
    [Google Scholar]
  21. Woisetschlaeger M., Jin M. X., Yandava C. N., Furmanski L. A., Strominger J. L., Speck S. H. 1991; Role for the Epstein-Barr virus nuclear antigen 2 in viral promoter switching during initial stages of infection of B-lymphoctyes. Proceedings of the National Academy of Sciences, USA 88:3942–3946
    [Google Scholar]
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