1887

Abstract

Previously, we have shown that E5a can induce ex pression of the c- gene in human papillomavirus (HPV)-11 E5a transformed NIH 3T3 cells and human epidermal keratinocytes. In this study, we investigated the relationship between expression of the E5a gene and c- in pathologically confirmed condylomata specimens using mRNA hybridization . The c- RNA concentration was significantly higher in condylomata specimens with E5a mRNA expression than in specimens without E5a mRNA expression, or in normal cervical specimens. The cells with c- expression were located predominantly in the basal and parabasal cell layers. These layers were also the primary location of E5a-expressing cells. This is the first demonstration of a strong correlation (74%) between expression of the E5a and c- genes in condylomata specimens. This correlation might reflect regulation by HPV-11 E5a of c- gene expression in condyloma.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-6-1145
1996-06-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/6/JV0770061145.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-6-1145&mimeType=html&fmt=ahah

References

  1. Angel P., Karin M. 1991; The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochimica et Biophysica Acta 1072:129–157
    [Google Scholar]
  2. Bodkin D. K., Knudson D. L. 1985; Assessment of sequence relatedness of double-stranded RNA genes by RNA-RNA blot hybridization. Journal of Virological Methods 10:45–52
    [Google Scholar]
  3. Bouvard V., Matlashewski G., Gu Z. M., Storey A., Banks L. 1994; The human papillomavirus type 16 E5 gene cooperates with the E7 gene to stimulate proliferation of primary cells and increases viral gene expression. Virology 203:73–80
    [Google Scholar]
  4. Brown D. B., Fan L., Jones J., Bryan J. 1994; Colocalization of human papillomavirus type 11 E1^E4 and L1 proteins in human foreskin implants grown in athymic mice. Virology 201:46–54
    [Google Scholar]
  5. Chen S. L., Mounts P. 1990; Transforming activity of E5a protein of human papillomavirus type 6 in NIH 3T3 cells and Cl27 cells. Journal of Virology 64:3226–3233
    [Google Scholar]
  6. Chen S. L., Han C. P., Tsao Y. P., Lee J. W., Yin C. S. 1993; Identification and typing of human papillomavirus in cervical cancers in Taiwan. Cancer 72:1939–1945
    [Google Scholar]
  7. Chen S. L., Tsao Y. P., Lee J. W., Liu H. S., Yang C. M., Tsao L. T. 1994a; E5a gene of human papillomavirus type 11 is required for initiation but not maintenance of transformation in NIH 3T3 cells. Journal of General Virology 75:1953–1960
    [Google Scholar]
  8. Chen S. L., Tsao L. T., Tsao Y. P. 1994b; Antisense oligodeoxynucleotides to c-jun inhibit proliferation of transformed NIH 3T3 cells induced by E5a of HPV-11. Cancer Letters 85:119–123
    [Google Scholar]
  9. Chen S. L., Tsao Y. P., Yang C. M., Lin Y. K., Huang C. H., Kao S. W. 1995; Differential induction and regulation of c-jun, junB, junD and c-fos by human papillomavirus type 11 E5a oncoprotein. Journal of General Virology 76:2653–2659
    [Google Scholar]
  10. Chow L. T., Nasseri M., Wolinsky S. M., Broker T. R. 1987; Human papillomavirus types 6 and 11 mRNAs from genital condyloma acuminata. Journal of Virology 61:2581–2588
    [Google Scholar]
  11. Cox K. H., Deleon D. V., Angerer L. M., Angerer R. C. 1984; Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Developmental Biology 101:485–502
    [Google Scholar]
  12. Hsieh T. B., Tsao Y. P., Yin C. S., Chen S. L. 1994; Detection of HPV6/11 DNA in anogenital condyloma acuminata by in situ hybridization. Journal of Obstetrics and Gynecology ROC 33:157–167
    [Google Scholar]
  13. Pfister H., Fuchs P. G. 1994; Anatomy, taxonomy and evolution of papillomaviruses. Intervirology 37:143–149
    [Google Scholar]
  14. Ryder K., Nathans D. 1988; Induction of protocogene c-jun by serum growth-factors. Proceedings of the National Academy of Science, USA 85:8464–8467
    [Google Scholar]
  15. Stoler M. H., Wolinsky S. M., Whitbeck A., Broker T. R., Chow L. T. 1989; Differentiation-linked human papillomavirus types 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message-specific RNA probes. Virology 172:331–340
    [Google Scholar]
  16. Stoler M. H., Rhodes C. R., Whitbeck A., Wolinsky S. M., Chow L. T., Broker T. R. 1992; Human papillomavirus type 16 and 18 gene expression in cervical neoplasias. Human Pathology 23:117–128
    [Google Scholar]
  17. Symonds R. P., Habeshaw T., Paul J., Kerr D. J., Darling A., Burnett R. A., Sotsiou R., Linardopoulos S., Spandidos D. A. 1992; No correlation between ras, c-myc, and c-jun protooncogene expression and prognosis in advanced carcimona of cervix. European Journal of Cancer 28A:1615–1617
    [Google Scholar]
  18. Tsao Y. P., Chu T. Y., Chen T. M., Yang Y. F., Chen S. L. 1994; The effects of E5a and E7 genes of human papillomavirus type 11 on immortalized human epidermal keratinocytes and NIH 3T3 cells. Archives of Virology 138:177–185
    [Google Scholar]
  19. Wilson J. L., Dollard S. C., Chow L. T., Broker T. R. 1992; Epithelial-specific gene expression during differentiation of stratified primary human keratinocyte cultures. Cell Growth and Differentiation 3:471–483
    [Google Scholar]
  20. Yang Y. F., Tsao Y. P., Yin C. S., Chen S. L., Chu T. Y. 1996; Overexpression of the proto-oncogene c-jun in association with low risk type specific human papillomavirus infection in condyloma acuminata. Journal of Medical Virology (in press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-6-1145
Loading
/content/journal/jgv/10.1099/0022-1317-77-6-1145
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error