1887

Abstract

Hepatitis B virus (HBV) wild-type pre-C RNA directs the synthesis of the HBeAg precursor but does not serve as mRNA for translation of the adjacent downstream C gene which encodes the core protein. Using bicistronic mRNA constructs that mimick pre-C RNA, we have demonstrated that this RNA likewise does not serve as messenger for translation of the P gene, which is located downstream of the C gene. However, when the pre-C RNA contains a translational stop codon at position 2 or 28 of the pre-C sequence (as in certain HBV mutants), it no longer directs synthesis of the HBeAg precursor but instead translation is initiated at downstream C and P gene AUGs. We propose that this occurs by a translation reinitiation mechanism.

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1996-06-01
2022-05-28
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References

  1. Bartenschlager R., Schaller H. 1992; Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome. EMBO Journal 11:3413–3420
    [Google Scholar]
  2. Bartenschlager R., Junker-Niepmann M., Schaller H. 1990; The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. Journal of Virology 64:5324–5332
    [Google Scholar]
  3. Bhat R. A., Ulrich P. P., Vyas G. N. 1990; Molecular characterization of a new variant of hepatitis B virus in a persistently infected homosexual male. Hepatology 11:271–276
    [Google Scholar]
  4. Brunetto M. R., Giarin M. M., Oliveri F., Chiaberge E., Baldi M., Alfarano A., Serra A., Saracco G., Verme G., Will H., Bonino F. 1991; Wild-type and e antigen-minus hepatitis B viruses and course of chronic hepatitis. Proceedings of the National Academy of Sciences, USA 88:4186–4190
    [Google Scholar]
  5. Carlier D., Jean-Jean O., Fouillot N., Will H., Rossignol J.-M. 1995; Importance of the C terminus of the hepatitis B virus precore protein in secretion of HBe antigen. Journal of General Virology 76:1041–1045
    [Google Scholar]
  6. Fouillot N., Tlouzeau S., Rossignol J.-M., Jean-Jean O. 1993; Translation of the hepatitis B virus P gene by ribosomal scanning as an alternative to internal initiation. Journal of Virology 67:4886–4895
    [Google Scholar]
  7. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., Howard B. H. 1982; The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eucaryotic cells by DNA-mediated transfection. Proceedings of the National Academy of Sciences, USA 79:6777–6781
    [Google Scholar]
  8. Hasegawa K., Huang J., Wands J. R., Obata H., Liang T. J. 1991; Association of hepatitis B viral precore mutations with fulminant hepatitis B in Japan. Virology 185:460–463
    [Google Scholar]
  9. Jean-Jean O., Levrero M., Will H., Perricaudet M., Rossignol J.-M. 1989a; Expression mechanism of the hepatitis B virus (HBV) C gene and biosynthesis of HBe antigen. Virology 170:99–106
    [Google Scholar]
  10. Jean-Jean O., Weimer T., De Recondo A. M., Will H., Rossignol J.-M. 1989b; Internal entry of ribosomes and ribosomal scanning involved in hepatitis B virus P gene expression. Journal of Virology 63:5451–5454
    [Google Scholar]
  11. Junker-Niepmann M., Bartenschlager R., Schaller H. 1990; A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO Journal 9:3389–3396
    [Google Scholar]
  12. Kozak M. 1987; Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Molecular and Cellular Biology 7:3438–3445
    [Google Scholar]
  13. Nassal M., Junker-Niepmann M., Schaller H. 1990; Translational inactivation of RNA function: discrimination against a subset of genomic transcripts during HBV nucleocapsid assembly. Cell 63:1357–1363
    [Google Scholar]
  14. Pollicino T., Campo S., Raimondo G. 1995; PreS and core gene heterogeneity in hepatitis B virus (HBV) genomes isolated from patients with long-lasting HBV chronic infection. Virology 208:672–677
    [Google Scholar]
  15. Schlicht H. J., von Brunn A., Theilmann L. 1991; Antibodies in anti-HBe-positive patient sera bind to an HBe protein expressed on the cell surface of hepatoma cells: implications for virus clearance. Hepatology 13:57–61
    [Google Scholar]
  16. Standring D. N., Ou J.-H., Masiarz F. R., Rutter W. J. 1988; A signal peptide encoded within the precore region of hepatitis B virus directs the secretion of a heterogeneous population of e antigens in Xenopus oocyctes. Proceedings of the National Academy of Sciences, USA 85:8405–8409
    [Google Scholar]
  17. Tong S. P., Li J. S., Vitvitski L., Trepo C. 1990; Active hepatitis B virus replication in the presence of anti-HBe is associated with viral variants containing an inactive pre-C region. Virology 176:596–603
    [Google Scholar]
  18. Will H., Reiser W., Weimer T., Pfaff E., Buscher M., Sprengel R., Cattaneo R., Schaller H. 1987; Replication strategy of human hepatitis B virus. Journal of Virology 61:904–911
    [Google Scholar]
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